Establishment and characterization of a new cell line derived from half-smooth tongue sole <italic style="font-style: italic">Cynoglossus semilaevis</italic> kidney

Yanan LOU; Bin SUN; Lina ZHANG; Yong LI; Peng XIAO

doi:10.1007/s00343-019-9084-y

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Establishment and characterization of a new cell line derived from half-smooth tongue sole Cynoglossus semilaevis kidney

Aquaculture and Fisheries | Updated：2023-04-27

- Establishment and characterization of a new cell line derived from half-smooth tongue sole Cynoglossus semilaevis kidney
- Establishment and characterization of a new cell line derived from half-smooth tongue sole Cynoglossus semilaevis kidney
- 海洋湖沼学报（英文） 2020年38卷第2期页码：560-570
- Affiliations：
  
  1.Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China
  2.Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China
  3.University of Chinese Academy of Sciences, Beijing 100049, China
  4.Tianjin Weishan Road Middle School, Tianjin 300222, China
- Author bio：
  
  XIAO Peng, E-mail:xiaopeng@qdio.ac.cn
- Funds：
  
  the National Natural Science Foundation of China(31672692);the Shinan District Project of Science and Technology Development Fund of Qingdao(2016-3-013-ZH);© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2020
- DOI：10.1007/s00343-019-9084-y
  中图分类号：
- 收稿：2019-03-26，
  
  录用：2019-5-5，
  
  网络首发：2019-07-29，
  
  纸质出版：2020-03
- Accepted：
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Establishment and characterization of a new cell line derived from half-smooth tongue sole Cynoglossus semilaevis kidney[J]. 海洋湖沼学报（英文）, 2020,38(2):560-570.

Yanan LOU, Bin SUN, Lina ZHANG, et al. Establishment and characterization of a new cell line derived from half-smooth tongue sole Cynoglossus semilaevis kidney[J]. Journal of Oceanology and Limnology, 2020, 38(2): 560-570.
Establishment and characterization of a new cell line derived from half-smooth tongue sole Cynoglossus semilaevis kidney[J]. 海洋湖沼学报（英文）, 2020,38(2):560-570. DOI： 10.1007/s00343-019-9084-y.

Yanan LOU, Bin SUN, Lina ZHANG, et al. Establishment and characterization of a new cell line derived from half-smooth tongue sole Cynoglossus semilaevis kidney[J]. Journal of Oceanology and Limnology, 2020, 38(2): 560-570. DOI： 10.1007/s00343-019-9084-y.

摘要

Abstract

In this study

we established a new cell line (

Cynoglossus semilaevis

kidney cells

CSK) from kidney of the half-smooth tongue sole (

Cynoglossus semilaevis

). The cells were subcultured over 1 000 days and passaged for more than 100 times. Additionally

CSK cells were optimally maintained in Dulbecco's modified Eagle medium nutrient mixture F-12 supplemented with HEPES

antibiotics

fetal bovine serum

2-mercaptoethanol

and basic fibroblast growth factor. The optimum growth temperature for CSK cells was 25℃

and the cells showed a fibroblast-like phenotype. Chromosome analysis revealed that CSK cells had a normal diploid karyotype with 2n=42. CSK cells were susceptible to Grouper nervous necrosis virus (NNV)

and cytopathic effects were observed at 3-5 days postinfection. The NNV sensitivity of CSK cells was related to the high abundance of virions in the cytoplasm

as observed by electron microscopy. Additionally

CSK cells could be successfully transfected with a green fluorescent protein reporter plasmid

and fluorescent signals were easily observed. Finally

immunocytochemistry analysis showed that CSK cells were supporting cells. Overall

we established this new cell line

which may have potential applications in the identification of viral pathogens affecting the half-smooth tongue sole.

关键词

Keywords

references

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Establishment and characterization of a new cell line derived from half-smooth tongue sole Cynoglossus semilaevis kidney

Establishment and characterization of a new cell line derived from half-smooth tongue sole Cynoglossus semilaevis kidney

DOI：10.1007/s00343-019-9084-y

摘要

Abstract

关键词

Keywords

references