Proof of homothally of <italic style="font-style: italic">Pheopolykrikos hartmannii</italic> and details of cyst germination process

Zhaoyang CHAI; Zhangxi HU; Yuyang LIU; Yingzhong TANG

doi:10.1007/s00343-019-9077-x

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Proof of homothally of Pheopolykrikos hartmannii and details of cyst germination process

Ecology | Updated：2023-04-27

- Proof of homothally of Pheopolykrikos hartmannii and details of cyst germination process
- Proof of homothally of Pheopolykrikos hartmannii and details of cyst germination process
- 海洋湖沼学报（英文） 2020年38卷第1期页码：114-123
- Affiliations：
  
  1.Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
  2.Laboratory of Marine Ecology and Environmental Science, Qingdao National Laboratory of Marine Science and Technology, Qingdao 266237, China
  3.Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China
  4.University of Chinese Academy of Sciences, Beijing 100049, China
- Author bio：
  
  TANG Yingzhong, yingzhong.tang@qdio.ac.cn
- Funds：
  
  the National Key R & D Program of China(2017YFC1404300);the National Natural Science Foundation of China(61533011);the National Natural Science Foundation of China(41776125);the NSFC-Shandong Joint Fund for Marine Ecology and Environmental Sciences(U1606404);the Science and Technology Basic Resources Investigation Program of China(2018FY100200);the Scientific and Technological Innovation Project of the Qingdao National Laboratory for Marine Science and Technology(2016ASKJ02);© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2020
- DOI：10.1007/s00343-019-9077-x
  中图分类号：
- 收稿：2019-03-21，
  
  录用：2019-4-26，
  
  网络首发：2019-05-15，
  
  纸质出版：2020-01
- Accepted：
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Proof of homothally of Pheopolykrikos hartmannii and details of cyst germination process[J]. 海洋湖沼学报（英文）, 2020,38(1):114-123.

Zhaoyang CHAI, Zhangxi HU, Yuyang LIU, et al. Proof of homothally of Pheopolykrikos hartmannii and details of cyst germination process[J]. Journal of Oceanology and Limnology, 2020, 38(1): 114-123.
Proof of homothally of Pheopolykrikos hartmannii and details of cyst germination process[J]. 海洋湖沼学报（英文）, 2020,38(1):114-123. DOI： 10.1007/s00343-019-9077-x.

Zhaoyang CHAI, Zhangxi HU, Yuyang LIU, et al. Proof of homothally of Pheopolykrikos hartmannii and details of cyst germination process[J]. Journal of Oceanology and Limnology, 2020, 38(1): 114-123. DOI： 10.1007/s00343-019-9077-x.

摘要

Abstract

Resting cysts play crucial roles in the ecology of dinoflagellates

especially in wintering or surviving unfavorable conditions

seeding harmful algal blooms (HABs)

and facilitating the geographic expansion. Encystment of dinoflagellates is tightly coupled with sexual reproduction in most cases

which can occur either through homothallism (self-fertilization) or heterothallism (intercrossing of +/-strains). The types of sexual reproduction have important ecological implications. The toxic and HAB-forming dinoflagellate

Pheopolykrikoides hartmannii

has been previously reported to be heterothallic. Here

we provide visual confirmation of homothally of

P. hartmannii

and the first detailed visual recording of cyst germination based on the observations of a clonal isolate from Jiaozhou Bay

China. To document the homothallism

we first observed cell pairs in sexual mating

planozygotes with two longitudinal flagella

and cysts with typical morphology as described previously from the clonal culture. We then germinated a single cyst

established a new clonal culture from one of the two daughter cells after the first cell division of the germling (i.e. from the diploid germling to two haploid cells)

and produced cysts again from the newly established clonal culture. For the observation of the germination time-series

we took micrographs and videos to show all germination processes

particularly with an interesting observation of the short amoeboid stage of the germling releasing from the archeopyle (~15 s)

which was a landmark of the germination process and has not been reported elsewhere. This definitive evidence of homothallic sexuality and cyst production in

P. hartmannii

provides a new insight into the biology and ecology of the species

particularly a mechanism that may partly account for the population dynamics and ubiquitous distribution of the species.

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Keywords

references

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DOI：10.1007/s00343-019-9077-x

摘要

Abstract

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