Biosynthesis of phycocyanobilin in recombinant <italic style="font-style: italic">Escherichia coli</italic>

MA Chengbo; LI Wenjun; GE Baosheng; LIN Jian; QIN Song

doi:10.1007/s00343-019-9060-6

Your Location：

Home >

Browse articles >

Biosynthesis of phycocyanobilin in recombinant Escherichia coli

Updated：2023-04-27

- Biosynthesis of phycocyanobilin in recombinant Escherichia coli
- Biosynthesis of phycocyanobilin in recombinant Escherichia coli
- 海洋湖沼学报（英文） 2020年38卷第2期页码：529-538
- Affiliations：
  
  1.College of Life Sciences, Yantai University, Yantai 264005, China
  2.Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China
  3.Center for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao 266580, China
  4.Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China
- Author bio：
  
  LIN Jian, linjian3384@163.com
  QIN Song, sqin@yic.ac.cn
- Funds：
  
  the National Natural Science Foundation of China(41906109);the Key Research and Development Program of Yantai(Y933021011);© Chinese Society for Oceanology and Limnology, Science Press, and Springer-Verlag Berlin Heidelberg 2020
- DOI：10.1007/s00343-019-9060-6
  中图分类号：
- 收稿：2019-03-12，
  
  录用：2019-5-8，
  
  网络首发：2019-08-14，
  
  纸质出版：2020-03
- Accepted：
Scan QR Code
Biosynthesis of phycocyanobilin in recombinant Escherichia coli[J]. 海洋湖沼学报（英文）, 2020,38(2):529-538.

MA Chengbo, LI Wenjun, GE Baosheng, et al. Biosynthesis of phycocyanobilin in recombinant Escherichia coli[J]. Journal of Oceanology and Limnology, 2020, 38(2): 529-538.
Biosynthesis of phycocyanobilin in recombinant Escherichia coli[J]. 海洋湖沼学报（英文）, 2020,38(2):529-538. DOI： 10.1007/s00343-019-9060-6.

MA Chengbo, LI Wenjun, GE Baosheng, et al. Biosynthesis of phycocyanobilin in recombinant Escherichia coli[J]. Journal of Oceanology and Limnology, 2020, 38(2): 529-538. DOI： 10.1007/s00343-019-9060-6.

摘要

Abstract

The recombinant expression of phycocyanobilin (PCB) was carried out in

Escherichia coli

and the best fermentation conditions of recombinant

E. coli

biosynthesized PCB are optimized in the response surface methodology to improve PCB production. The recombinant PCB is extracted

isolated

and purified by methanol and chloroform extraction. Recombinant PCB is validated in UV-vis spectroscopy

high-pressure liquid chromatography

and mass spectrometry. In addition

the anti-oxidant activities of the recombinant PCB are determined. The best induction conditions that optimized by Design Expert 8.0 software include:lactose concentration 4 mmol/L

induction temperature 24.69℃

induction time 4.6 h

and induction duration 13.57 h

under which the PCB expression level reached approximately 13 mg PCB/L

which is more than four times of previously reported 3 mg PCB/L. The maximum absorption peak of the recombinant PCB is located at 680 nm with a high fluorescence intensity of 470 nm. The recombinant PCB has a good ability to scavenge 2

2-diphenyl-1-picrylhydrazyl (DPPH) free radicals.

关键词

Keywords

references

N Adir . Elucidation of the molecular structures of components of the phycobilisome: reconstructing a giant . Photosynthesis Research , 2005 . 85 ( 1 ): 15 - 32 . .

R M Alvey , A Biswas , W M Schluchter , D A Bryant . Attachment of noncognate chromophores to CpcA of Synechocystis sp . PCC 6803 and Synechococcus sp. PCC 7002 by heterologous expression in Escherichia coli. Biochemistry , 2011 . 50 ( 22 ): 4890 - 4902 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=d208f68aed40a763c3383ba9f7088f95 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=d208f68aed40a763c3383ba9f7088f95 , .

S A Basdeo , N K Campbell , L M Sullivan , B Flood , E M Creagh , T J Mantle , J M Fletcher , A Dunne . Suppression of human alloreactive T cells by linear tetrapyrroles; relevance for transplantation . Translational Research , 2016 . 178 81 - 94 . DOI: 10.1016/j.trsl.2016.07.011 http://doi.org/10.1016/j.trsl.2016.07.011 .

S Benedetti , F Benvenuti , S Scoglio , F Canestrari . Oxygen radical absorbance capacity of phycocyanin and phycocyanobilin from the food supplement Aphanizomenon flos - aquae . Journal of Medicinal Food , 2010 . 13 ( 1 ): 223 - 227 . DOI: 10.1089/jmf.2008.0257 http://doi.org/10.1089/jmf.2008.0257 .

A Bennett , L Bogorad . Complementary chromatic adaptation in a filamentous blue-green alga . Journal of Cell Biology , 1973 . 58 ( 2 ): 419 - 435 . DOI: 10.1083/jcb.58.2.419 http://doi.org/10.1083/jcb.58.2.419 .

R J Beuhler , R C Pierce , L Friedman , H W Siegelman . Cleavage of phycocyanobilin from C-phycocyanin . Separation and mass spectral identification of the products. Journal of Biological Chemistry , 1976 . 251 ( 8 ): 2405 - 2411 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=10.1515/znc-1980-9-1018 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=10.1515/znc-1980-9-1018 , .

M S Blois . Antioxidant determinations by the use of a stable free radical . Nature , 1958 . 181 ( 4617 ): 1199 - 1200 . DOI: 10.1038/1811199a0 http://doi.org/10.1038/1811199a0 .

P ó Carra , C ó Heocha . Bilins released from algae and biliproteins by methanolic extraction . Phytochemistry , 1966 . 5 ( 5 ): 993 - 997 . DOI: 10.1016/S0031-9422(00)82796-X http://doi.org/10.1016/S0031-9422(00)82796-X .

P R O De Montellano . The mechanism of heme oxygenase . Current Opinion in Chemical Biology , 2000 . 4 ( 2 ): 221 - 227 . DOI: 10.1016/S1367-5931(99)00079-4 http://doi.org/10.1016/S1367-5931(99)00079-4 .

N Frankenberg , J C Lagarias . Phycocyanobilin: ferredoxin oxidoreductase of Anabaena sp . PCC 7120 biochemical and spectroscopic characterization. Journal of Biological Chemistry , 2003 . 278 ( 11 ): 9219 - 9226 . http://d.old.wanfangdata.com.cn/NSTLQK/NSTL_QKJJ0212484336/ http://d.old.wanfangdata.com.cn/NSTLQK/NSTL_QKJJ0212484336/ , .

G A Gambetta , J C Lagarias . Genetic engineering of phytochrome biosynthesis in bacteria . Proceedings of the National Academy of Sciences of the United States of America , 2001 . 98 ( 19 ): 10566 - 10571 . DOI: 10.1073/pnas.191375198 http://doi.org/10.1073/pnas.191375198 .

B S Ge , Y Chen , Q Yu , X J Lin , J Q Li , S Qin . Regulation of the heme biosynthetic pathway for combinational biosynthesis of phycocyanobilin in Escherichia coli . Process Biochemistry , 2018 . 71 23 - 30 . DOI: 10.1016/j.procbio.2018.05.011 http://doi.org/10.1016/j.procbio.2018.05.011 .

B S Ge , Y Li , H X Sun , S Zhang , P J Hu , S Qin , F Huang . Combinational biosynthesis of phycocyanobilin using genetically-engineered Escherichia coli . Biotechnology Letters , 2013 . 35 ( 5 ): 689 - 693 . DOI: 10.1007/s10529-012-1132-z http://doi.org/10.1007/s10529-012-1132-z .

A N Glazer , S Fang . Chromophore content of blue-green algal phycobiliproteins . Journal of Biological Chemistry , 1973 . 248 ( 2 ): 659 - 662 . .

A Jobe , S Bourgeois . Lac repressor-operator interaction: VI . The natural inducer of the lac operon. Journal of Molecular Biology , 1972 . 69 ( 3 ): 397 - 408 . http://d.old.wanfangdata.com.cn/NSTLQK/NSTL_QKJJ0212888956/ http://d.old.wanfangdata.com.cn/NSTLQK/NSTL_QKJJ0212888956/ , .

K M Lee , D F Gilmore . Statistical experimental design for bioprocess modeling and optimization analysis . Applied Biochemistry and Biotechnology , 2006 . 135 ( 2 ): 101 - 115 . .

B Müller-Hill , H V Rickenberg , K Wallenfels . Specificity of the induction of the enzymes of the lac operon in Escherichia coli . Journal of Molecular Biology , 1964 . 10 ( 2 ): 303 - 318 . DOI: 10.1016/S0022-2836(64)80049-8 http://doi.org/10.1016/S0022-2836(64)80049-8 .

R MacColl . Cyanobacterial phycobilisomes . Journal of Structural Biology , 1998 . 124 ( 2-3 ): 311 - 334 . DOI: 10.1006/jsbi.1998.4062 http://doi.org/10.1006/jsbi.1998.4062 .

J Marín-Prida , N Pavón-Fuentes , A Llópiz-Arzuaga , J R Fernández-Massó , L Delgado-Roche , Y Mendoza-Marí , S P Santana , A Cruz-Ramírez , C Valenzuela-Silva , M Nazábal-Gálvez , A Cintado-Benítez , G L Pardo-Andreu , N Polentarutti , F Riva , E Pentón-Arias , G Pentón-Rol . Phycocyanobilin promotes PC12 cell survival and modulates immune and inflammatory genes and oxidative stress markers in acute cerebral hypoperfusion in rats . Toxicology and Applied Pharmacology , 2013 . 272 ( 1 ): 49 - 60 . DOI: 10.1016/j.taap.2013.05.021 http://doi.org/10.1016/j.taap.2013.05.021 .

D McCann , A Barrett , A Cooper , D Crumpler , L Dalen , K Grimshaw , E Kitchin , K Lok , L Porteous , E Prince , E Sonuga-Barke , J O Warner , J Stevenson . Food additives and hyperactive behaviour in 3-year-old and 8/9-year-old children in the community: a randomised, double-blinded, placebo-controlled trial . The Lancet , 2007 . 370 ( 9598 ): 1560 - 1567 . DOI: 10.1016/S0140-6736(07)61306-3 http://doi.org/10.1016/S0140-6736(07)61306-3 .

M F Mccarty , J Barroso-Aranda , F Contreras . NAPDH oxidase mediates glucolipotoxicity-induced beta cell dysfunction-clinical implications . Medical Hypotheses , 2010a . 74 ( 3 ): 596 - 600 . DOI: 10.1016/j.mehy.2008.09.062 http://doi.org/10.1016/j.mehy.2008.09.062 .

M F McCarty , J Barroso-Aranda , F Contreras . Oral phycocyanobilin may diminish the pathogenicity of activated brain microglia in neurodegenerative disorders . Medical Hypotheses , 2010b . 74 ( 3 ): 601 - 605 . DOI: 10.1016/j.mehy.2008.09.061 http://doi.org/10.1016/j.mehy.2008.09.061 .

P O'Carra , R F Murphy , S D Killilea . The native forms of the phycobilin chromophores of algal biliproteins . A clarification. Biochemical Journal , 1980 . 187 ( 2 ): 303 - 309 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=PubMed000001013505 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=PubMed000001013505 , .

H Qaiser , F Aslam , S Iftikhar , A Farooq . Construction and recombinant expression of Pseudomonas aeruginosa truncated exotoxin A in Escherichia coli . Cellular and Molecular Biology , 2018 . 64 ( 1 ): 64 - 69 . DOI: 10.14715/cmb/2018.64.1.12 http://doi.org/10.14715/cmb/2018.64.1.12 .

C Romay , J Armesto , D Remirez , R González , N Ledon , N L García . Antioxidant and anti-inflammatory properties of C-phycocyanin from blue-green algae . Inflammation Research , 1998 . 47 ( 1 ): 36 - 41 . DOI: 10.1007/s000110050256 http://doi.org/10.1007/s000110050256 .

C Romay , R González , N Ledón , D Remirez , V Rimbau . C-phycocyanin: a biliprotein with antioxidant, anti-inflammatory and neuroprotective eﬀects . Current Protein & Peptide Science , 2003 . 4 ( 3 ): 207 - 216 . http://d.old.wanfangdata.com.cn/Periodical/gpxygpfx200805036 http://d.old.wanfangdata.com.cn/Periodical/gpxygpfx200805036 , .

T G Sotiroudis , G T Sotiroudis . Health aspects of Spirulina ( Arthrospira ) microalga food supplement . Journal of the Serbian Chemical Society , 2013 . 78 ( 3 ): 395 - 405 . DOI: 10.2298/JSC121020152S http://doi.org/10.2298/JSC121020152S .

R D Willows , S M Mayer , M S Foulk , A DeLong , K Hanson , J Chory , S I Beale . Phytobilin biosynthesis: the Synechocystis sp . PCC 6803 heme oxygenase-encoding ho1 gene complements a phytochrome-deficient Arabidopsis thaliana hy1 mutant. Plant Molecular Biology , 2000 . 43 ( 1 ): 113 - 120 . http://d.old.wanfangdata.com.cn/NSTLQK/NSTL_QKJJ021075471/ http://d.old.wanfangdata.com.cn/NSTLQK/NSTL_QKJJ021075471/ , .

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

S-nitrosylation coordination enhances high salt tolerance in Ulva prolifera

Response surface optimization of preparation parameters and evaluation of the antioxidant and antihypertensive activities of Spirulina protein hydrolysates

Effects of dietary cottonseed meal protein hydrolysate on growth, antioxidants and immunity of Chinese mitten crab Eriocheir Sinensis

Effects of florfenicol on Cryptocaryon irritans infections in large yellow croaker in transcriptomics and metabolomics

压电阻抗法表征固体推进剂老化的研究

Biosynthesis of phycocyanobilin in recombinant Escherichia coli

Biosynthesis of phycocyanobilin in recombinant Escherichia coli

DOI：10.1007/s00343-019-9060-6

摘要

Abstract

关键词

Keywords

references