Transcriptomic analysis reveals putative osmoregulation mechanisms in the kidney of euryhaline turbot <italic style="font-style: italic">Scophthalmus maximus</italic> responded to hypo-saline seawater

Wenxiao CUI; Aijun MA; Zhihui HUANG; Xin'an WANG; Zhibin SUN; Zhifeng LIU; GWei ZHAN; Jingkun YANG; Jinsheng ZHANG; Jiangbo QU

doi:10.1007/s00343-019-9056-2

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Transcriptomic analysis reveals putative osmoregulation mechanisms in the kidney of euryhaline turbot Scophthalmus maximus responded to hypo-saline seawater

Updated：2023-04-27

- Transcriptomic analysis reveals putative osmoregulation mechanisms in the kidney of euryhaline turbot Scophthalmus maximus responded to hypo-saline seawater
- Transcriptomic analysis reveals putative osmoregulation mechanisms in the kidney of euryhaline turbot Scophthalmus maximus responded to hypo-saline seawater
- 海洋湖沼学报（英文） 2020年38卷第2期页码：467-479
- Affiliations：
  
  1.College of Fisheries and Life Science, Shanghai Ocean University, Ministry of Education, Shanghai 201306, China
  2.Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China
  3.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences; Shandong Key Laboratory of Marine Fisheries Biotechnology and Genetic Breeding; Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Qingdao 266071, China
  4.Yantai Tianyuan Aquatic Limited Corporation, Yantai 264003, China
- Author bio：
  
  MA Aijun, maaj@ysfri.ac.cn
- Funds：
  
  the Earmarked Fund for Modern Agro-Industry Technology Research System(CARS-47-G01);the AoShan Talents Cultivation Program supported by Qingdao National Laboratory for Marine Science and Technology(2017ASTCP-OS04);the National Natural Science Foundation of China(41706168);the Agricultural Fine Breed Project of Shandong(2019LZGC013);the Basal Research Fund, Chinese Academy of Fishery Sciences(2016HY-JC0301);the Yantai Science and Technology Project(2018ZDCX021);© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2020
- DOI：10.1007/s00343-019-9056-2
  中图分类号：
- 收稿：2019-03-08，
  
  录用：2019-7-10，
  
  网络首发：2019-07-22，
  
  纸质出版：2020-02
- Accepted：
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Transcriptomic analysis reveals putative osmoregulation mechanisms in the kidney of euryhaline turbot Scophthalmus maximus responded to hypo-saline seawater[J]. 海洋湖沼学报（英文）, 2020,38(2):467-479.

Wenxiao CUI, Aijun MA, Zhihui HUANG, et al. Transcriptomic analysis reveals putative osmoregulation mechanisms in the kidney of euryhaline turbot Scophthalmus maximus responded to hypo-saline seawater[J]. Journal of Oceanology and Limnology, 2020, 38(2): 467-479.
Transcriptomic analysis reveals putative osmoregulation mechanisms in the kidney of euryhaline turbot Scophthalmus maximus responded to hypo-saline seawater[J]. 海洋湖沼学报（英文）, 2020,38(2):467-479. DOI： 10.1007/s00343-019-9056-2.

Wenxiao CUI, Aijun MA, Zhihui HUANG, et al. Transcriptomic analysis reveals putative osmoregulation mechanisms in the kidney of euryhaline turbot Scophthalmus maximus responded to hypo-saline seawater[J]. Journal of Oceanology and Limnology, 2020, 38(2): 467-479. DOI： 10.1007/s00343-019-9056-2.

摘要

Abstract

Turbot harbor a relatively remarkable ability to adapt to opposing osmotic challenges and are an excellent model species to study the physiological adaptations of flounder associated with osmoregulatory plasticity. The kidney transcriptome of turbot treated 24 h in water of hypo-salinity (salinity 5) and seawater (salinity 30) was sequenced and characterized. In silico analysis indicated that all unigenes had significant hits in seven databases. The functional annotation analysis of the transcriptome showed that the immune system and biological processes associated with digestion

absorption

and metabolism played an important role in the osmoregulation of turbot in response to hypo-salinity. Analysis of biological processes associated with inorganic channels and transporters indicated that mineral absorption and bile secretion contributed to iono-osmoregulation resulting in cell volume regulation and cell phenotypic plasticity. Moreover

we analyzed and predicted the mechanisms of canonical signaling transduction. Biological processes involved in renin secretion

ECM-receptor interaction

adherens junction

and focal adhesion played an important role in the plasticity phenotype in hypo-stress

while the signal transduction network composed of the MAPK signaling pathway and PI3K-Akt signaling pathway with GABAergic synapse

worked in hypoosmoregulation signal transduction in the turbot. In addition

analysis of the tissue specificity of targeted gene expression using qPCR during salinity stress was carried out. The results showed that the kidney

gill

and spleen were vital regulating organs of osmotic pressure

and the osmoregulation pattern of euryhaline fish differed among species.

关键词

Keywords

references

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Transcriptomic analysis reveals putative osmoregulation mechanisms in the kidney of euryhaline turbot Scophthalmus maximus responded to hypo-saline seawater

DOI：10.1007/s00343-019-9056-2

摘要

Abstract

关键词

Keywords

references