PI3K/Akt pathway is involved in the activation of RAW 264.7 cells induced by hydroxypropyltrimethyl ammonium chloride chitosan

Yue YANG; Rong'e XING; Song LIU; Yukun QIN; Kecheng LI; Huahua YU; Pengcheng LI

doi:10.1007/s00343-019-9013-0

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PI3K/Akt pathway is involved in the activation of RAW 264.7 cells induced by hydroxypropyltrimethyl ammonium chloride chitosan

Updated：2023-05-19

- PI3K/Akt pathway is involved in the activation of RAW 264.7 cells induced by hydroxypropyltrimethyl ammonium chloride chitosan
- PI3K/Akt pathway is involved in the activation of RAW 264.7 cells induced by hydroxypropyltrimethyl ammonium chloride chitosan
- 海洋湖沼学报（英文） 2020年38卷第3期页码：834-840
- Affiliations：
  
  1.Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
  2.Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China
  3.University of Chinese Academy of Sciences, Beijing 100049, China
- Author bio：
  
  XING Rong'e, xingronge@qdio.ac.cn
  LI Pengcheng, pcli@qdio.ac.cn
- Funds：
  
  the National Key R&D Program of China(2018YFC0311305);the Key Research and Development Program of Shandong Province(2019GHY112015);the Key Research and Development Program of Shandong Province(2019YYSP028);© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2020
- DOI：10.1007/s00343-019-9013-0
  中图分类号：
- 收稿：2019-01-19，
  
  录用：2019-6-28，
  
  网络首发：2019-09-08，
  
  纸质出版：2020-03
- Accepted：
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PI3K/Akt pathway is involved in the activation of RAW 264.7 cells induced by hydroxypropyltrimethyl ammonium chloride chitosan[J]. 海洋湖沼学报（英文）, 2020,38(3):834-840.

Yue YANG, Rong'e XING, Song LIU, et al. PI3K/Akt pathway is involved in the activation of RAW 264.7 cells induced by hydroxypropyltrimethyl ammonium chloride chitosan[J]. Journal of Oceanology and Limnology, 2020, 38(3): 834-840.
PI3K/Akt pathway is involved in the activation of RAW 264.7 cells induced by hydroxypropyltrimethyl ammonium chloride chitosan[J]. 海洋湖沼学报（英文）, 2020,38(3):834-840. DOI： 10.1007/s00343-019-9013-0.

Yue YANG, Rong'e XING, Song LIU, et al. PI3K/Akt pathway is involved in the activation of RAW 264.7 cells induced by hydroxypropyltrimethyl ammonium chloride chitosan[J]. Journal of Oceanology and Limnology, 2020, 38(3): 834-840. DOI： 10.1007/s00343-019-9013-0.

摘要

Abstract

We previously demonstrated that 2-hydroxypropyltrimethyl ammonium chloride chitosan (HACC) promoted the production of nitric oxide (NO) and proinflammatory cytokines by activating the mitogen-activated protein kinases (MAPK) and Janus kinase (JAK)/STAT pathways in RAW 264.7 cells

indicating good immunomodulatory activity of HACC. In this study

to further investigate the immunomodulatory mechanisms of HACC

we determined the roles of phosphatidylinositol 3-kinase (PI3K)/Akt

activating protein (AP-1) and nuclear factor kappa B (NF-κB) in HACC-induced activation of RAW 264.7 cells by the western blotting. The results suggest that HACC promoted the phosphorylation of p85 and Akt. Furthermore

c-Jun and p65 were also increased after the treatment of RAW 264.7 cells with HACC

indicating the translocation of NF-κB and AP-1 from cytoplasm to nucleus. In addition

as scanning electron microscopy (SEM) analysis shows

the cell morphology changed after HACC treatment. These findings indicate that HACC activated MAPK

JAK/STAT

and PI3K/Akt signaling pathways dependent on AP-1 and NF-κB activation in RAW 264.7 cells

ultimately leading to the increase of NO and cytokines.

关键词

Keywords

references

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