Integrated transcriptome and physiology analysis of <italic style="font-style: italic">Microcystis aeruginosa</italic> after exposure to copper sulfate

Tao WANG; Yadong HU; Ming ZHU; Shaowu YIN

doi:10.1007/s00343-019-8357-9

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Integrated transcriptome and physiology analysis of Microcystis aeruginosa after exposure to copper sulfate

Ecology | Updated：2023-04-27

- Integrated transcriptome and physiology analysis of Microcystis aeruginosa after exposure to copper sulfate
- Integrated transcriptome and physiology analysis of Microcystis aeruginosa after exposure to copper sulfate
- 海洋湖沼学报（英文） 2020年38卷第1期页码：102-113
- Affiliations：
  
  1.College of Marine Science and Engineering, College of Life Science, Nanjing Normal University, Nanjing 210023, China
  2.Co-Innovation Center for Marine Bio-Industry Technology of Jiangsu Province, Lianyungang 222005, China
  3.College of Marine Life and Mariculture, Huaihai Institute of Technology, Lianyungang 222005, China
  4.Nanjing Ocean-wisdom Biotech Company, Nanjing 211132, China
- Author bio：
  
  YIN Shaowu, yinshaowu@163.com
- Funds：
  
  the Natural Science Foundation of Jiangsu Province of China(BK20180728);the Key Research and Development Program of Jiangsu Province(BE2017377);the Creation Project of Major New Species of Agriculture in Jiangsu Province(PZCZ201742);© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2020
- DOI：10.1007/s00343-019-8357-9
  中图分类号：
- 收稿：2018-12-17，
  
  录用：2019-3-8，
  
  网络首发：2019-04-15，
  
  纸质出版：2020-01
- Accepted：
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Integrated transcriptome and physiology analysis of Microcystis aeruginosa after exposure to copper sulfate[J]. 海洋湖沼学报（英文）, 2020,38(1):102-113.

Tao WANG, Yadong HU, Ming ZHU, et al. Integrated transcriptome and physiology analysis of Microcystis aeruginosa after exposure to copper sulfate[J]. Journal of Oceanology and Limnology, 2020, 38(1): 102-113.
Integrated transcriptome and physiology analysis of Microcystis aeruginosa after exposure to copper sulfate[J]. 海洋湖沼学报（英文）, 2020,38(1):102-113. DOI： 10.1007/s00343-019-8357-9.

Tao WANG, Yadong HU, Ming ZHU, et al. Integrated transcriptome and physiology analysis of Microcystis aeruginosa after exposure to copper sulfate[J]. Journal of Oceanology and Limnology, 2020, 38(1): 102-113. DOI： 10.1007/s00343-019-8357-9.

摘要

Abstract

Copper sulfate (CuSO

) is widely used in controlling the

Microcystis aeruginosa

blooms. Many studies reported the toxicity mechanisms of Cu

M. aeruginosa

at the physiological level

but little is known about a transcriptomic basis of these mechanisms. In the present study

M. aeruginosa

was treated by 0.5 mg/L Cu

(half of the 96-h EC

) for 72 h. The results show that Cu

content in

M. aeruginosa

increased after 72 h Cu

exposure

whereas the

chlorophyll fluorescence value and chlorophyll a content in

M. aeruginosa

sharply decreased. Reactive oxygen species concentration and activity of antioxidant enzymes (superoxide dismutase

catalase and peroxidase) were all increased. These physiological data confirmed toxicity of Cu

M. aeruginosa

. The RNA-seq analysis showed that 6 646 725 and 7 880 291 clean reads were obtained for the Cu-treated and control libraries

respectively. The 595 genes (252 downward trend and 343 upward trend) with the Gene Ontology (GO) annotations were divided into three main functional categories:cellular component

molecular function

and biological process. In the Cluster of Orthologous Groups (COG) annotation

418 differentially expressed genes with 25 functional definitions were obtained. Among them

'replication

recombination and repair'

'energy production and conversion'

and 'general function prediction only' were the largest three groups of transcripts. In the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis

genes involved in photosynthesis and oxidative phosphorylation were present at the highest percentages. In addition

the genes involved in photosynthesis and oxidative phosphorylation were identified

and then confirmed using real-time PCR. This study reported the first transcriptome of

M. aeruginosa

. Photosynthesis and oxidative phosphorylation were severely affected by Cu

toxicity

which may have contributed to cell death. These data provide the potential mechanism to explain the CuSO

effect on the harmful

M. aeruginosa

关键词

Keywords

references

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Integrated transcriptome and physiology analysis of Microcystis aeruginosa after exposure to copper sulfate

Integrated transcriptome and physiology analysis of Microcystis aeruginosa after exposure to copper sulfate

DOI：10.1007/s00343-019-8357-9

摘要

Abstract

关键词

Keywords

references