Transcriptional responses to starvation of pathogenic <italic style="font-style: italic">Vibrio harveyi</italic> strain DY1

Xiaodan LIU; Xiaojian GAO; Nan CHEN; Yingying ZHANG; Xixi LI; Yue ZHANG; Xiaojun ZHANG

doi:10.1007/s00343-019-8350-3

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Transcriptional responses to starvation of pathogenic Vibrio harveyi strain DY1

Aquaculture and Fisheries | Updated：2023-04-27

- Transcriptional responses to starvation of pathogenic Vibrio harveyi strain DY1
- Transcriptional responses to starvation of pathogenic Vibrio harveyi strain DY1
- 海洋湖沼学报（英文） 2020年38卷第2期页码：579-587
- Affiliations：
  
  College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China
- Author bio：
  
  ZHANG Xiaojun, zxj9307@163.com
- Funds：
  
  the Jiangsu Agricultural Industry Technology System(02);the National Natural Science Foundation of China(31972830);the Natural Science Foundation of Jiangsu Province(BK20180915);the Jiangsu Agriculture Science and Technology Innovation Fund(CX(18)2012);the Fishery Science and Technology Innovation Projects of Jiangsu Province(D2017-3);the "Blue Project" of Yangzhou University and the Projects of Shuichan Sanxin of Jiangsu Province(Y2016-33);© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2020
- DOI：10.1007/s00343-019-8350-3
  中图分类号：
- 收稿：2019-01-04，
  
  录用：2019-4-28，
  
  网络首发：2019-07-16，
  
  纸质出版：2020-02
- Accepted：
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Transcriptional responses to starvation of pathogenic Vibrio harveyi strain DY1[J]. 海洋湖沼学报（英文）, 2020,38(2):579-587.

Xiaodan LIU, Xiaojian GAO, Nan CHEN, et al. Transcriptional responses to starvation of pathogenic Vibrio harveyi strain DY1[J]. Journal of Oceanology and Limnology, 2020, 38(2): 579-587.
Transcriptional responses to starvation of pathogenic Vibrio harveyi strain DY1[J]. 海洋湖沼学报（英文）, 2020,38(2):579-587. DOI： 10.1007/s00343-019-8350-3.

Xiaodan LIU, Xiaojian GAO, Nan CHEN, et al. Transcriptional responses to starvation of pathogenic Vibrio harveyi strain DY1[J]. Journal of Oceanology and Limnology, 2020, 38(2): 579-587. DOI： 10.1007/s00343-019-8350-3.

摘要

Abstract

Vibrio harveyi

is a pathogen of various aquatic organisms that has been recently associated with massive mortality episodes in the aquaculture industry. Recurrent outbreaks of vibriosis are closely correlated with the capacity of this bacterial species to survive long-term starvation conditions. To study the regulation mechanism of gene expression at the transcriptional level in

V. harveyi

under starvation conditions

the transcriptomic response profiles were determined of the Portunus trituberculatus pathogen

V. harveyi

strain DY1 under normal conditions and after four weeks of starvation. A total of 4 679 and 4 661 genes were expressed in the non-starved and starved cells

respectively. The significantly differentially expressed genes (DEGs) between non-starved and starved groups were identified

in which 255 genes were up-regulated and 411 genes were down-regulated. GO analysis and KEGG enrichment analysis were used to analyze the DEGs and revealed the involvement of these DEGs in many pathways

including ABC transporters

flagellum assembly

and fatty acid metabolism. Several DEGs were randomly selected and their expression levels were confirmed by quantitative real-time PCR (qRT-PCR). This is the first comprehensive transcriptomic analysis of starvation effects in

V. harveyi

. Our findings will facilitate future study on stress adaptation and survival mechanisms of

V. harveyi

关键词

Keywords

references

E Alcaide , C Gil-Sanz , E Sanjuán , D Esteve , C Amaro , L Silveira . Vibrio harveyi causes disease in seahorse, Hippocampus sp . Journal of Fish Diseases , 2010 . 24 ( 5 ): 311 - 313 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=10.1046/j.1365-2761.2001.00297.x http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=10.1046/j.1365-2761.2001.00297.x , .

C R Arias , S LaFrentz , W L Cai , O Olivares-Fuster . Adaptive response to starvation in the fish pathogen flavobacterium columnare : cell viability and ultrastructural changes . BMC Microbiology , 2012 . 12 266 DOI: 10.1186/1471-2180-12-266 http://doi.org/10.1186/1471-2180-12-266 .

B Austin , X H Zhang . Vibrio harveyi : a significant pathogen of marine vertebrates and invertebrates . Letters in Applied Microbiology , 2006 . 43 ( 2 ): 119 - 124 . .

B Austin . Vibrios as causal agents of zoonoses . Veterinary Microbiology , 2010 . 140 ( 3-4 ): 310 - 317 . DOI: 10.1016/j.vetmic.2009.03.015 http://doi.org/10.1016/j.vetmic.2009.03.015 .

E G Biosca , C Amaro , E Marco-Noales , J D Oliver . Effect of low temperature on starvation-survival of the eel pathogen Vibrio vulnificus biotype 2 . Applied and Environmental Microbiology , 1996 . 62 ( 2 ): 450 - 455 . DOI: 10.1128/AEM.62.2.450-455.1996 http://doi.org/10.1128/AEM.62.2.450-455.1996 .

R Company , A Sitj-Bobadilla , M J Pujalte , E Garay , P AlvarezPellitero . Bacterial and parasitic pathogens in cultured common dentex, Dentex dentex L . Journal of Fish Diseases , 1999 . 22 ( 4 ): 299 - 309 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=10.1046/j.1365-2761.1999.00182.x http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=10.1046/j.1365-2761.1999.00182.x , .

B K Diggles , G A Moss , J Carson , C D Anderson . Luminous vibriosis in rock lobster Jasus verreauxi (Decapoda: Palinuridae) phyllosoma larvae associated with infection by Vibrio harveyi . Diseases of Aquatic Organisms , 2000 . 43 ( 2 ): 127 - 137 . .

E Gauger , R Smolowitz , K Uhlinger , J Casey , M Gómez-Chiarri . Vibrio harveyi and other bacterial pathogens in cultured summer flounder, Paralichthys dentatus . Aquaculture , 2006 . 260 ( 1-4 ): 10 - 20 . DOI: 10.1016/j.aquaculture.2006.06.012 http://doi.org/10.1016/j.aquaculture.2006.06.012 .

S Haldar , A Maharajan , S Chatterjee , S A Hunter , N Chowdhury , A Hinenoya , M Asakura , S Yamasaki . Identification of Vibrio harveyi as a causative bacterium for a tail rot disease of sea bream Sparus aurata from research hatchery in Malta . Microbiological Research , 2010 . 165 ( 8 ): 639 - 648 . DOI: 10.1016/j.micres.2009.12.001 http://doi.org/10.1016/j.micres.2009.12.001 .

C F Higgins . ABC transporters: physiology, structure and mechanism—an overview . Research in Microbiology , 2001 . 152 ( 3-4 ): 205 - 210 . DOI: 10.1016/S0923-2508(01)01193-7 http://doi.org/10.1016/S0923-2508(01)01193-7 .

C Hispano , Y Nebra , A R Blanch . Isolation of Vibrio harveyi from an ocular lesion in the short sunfish ( Mola mola ) . Bulletin of the European Association of Fish Pathologists , 1997 . 17 ( 3-4 ): 104 - 107 . .

X Jiang , T J Chai . Survival of Vibrio parahaemolyticus at low temperatures under starvation conditions and subsequent resuscitation of viable, nonculturable cells . Applied and Environmental Microbiology , 1996 . 62 ( 4 ): 1 300 - 1 305 . DOI: 10.1128/AEM.62.4.1300-1305.1996 http://doi.org/10.1128/AEM.62.4.1300-1305.1996 .

F H Johnson , I V Shunk . An interesting new species of luminous bacteria . Journal of Bacteriology , 1936 . 31 ( 6 ): 585 - 593 . DOI: 10.1128/JB.31.6.585-593.1936 http://doi.org/10.1128/JB.31.6.585-593.1936 .

V R Kaberdin , U Bläsi . Translation initiation and the fate of bacterial mRNAs . FEMS Microbiology Reviews , 2006 . 30 ( 6 ): 967 - 979 . DOI: 10.1111/j.1574-6976.2006.00043.x http://doi.org/10.1111/j.1574-6976.2006.00043.x .

V R Kaberdin , I Montánchez , C Parada , M Orruño , I Arana , I Barcina . Unveiling the metabolic pathways associated with the adaptive reduction of cell size during vibrio harveyi persistence in seawater microcosms . Microbial Ecology , 2015 . 70 ( 3 ): 689 - 700 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=80eaad2d99e37acf945113811665184d http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=80eaad2d99e37acf945113811665184d , .

C Kim , M Mwangi , M Chung , C Milheirço , H De Lencastre , A Tomasz . The mechanism of heterogeneous betalactam resistance in MRSA: key role of the stringent stress response . PLoS One , 2013 . 8 ( 12 ): e82814 DOI: 10.1371/journal.pone.0082814 http://doi.org/10.1371/journal.pone.0082814 .

S Kjellerberg , B A Humphrey , K C Marshall . Initial phases of starvation and activity of bacteria at surfaces . Applied and Environmental Microbiology , 1983 . 46 ( 5 ): 978 - 984 . DOI: 10.1128/AEM.46.5.978-984.1983 http://doi.org/10.1128/AEM.46.5.978-984.1983 .

T Kraxberger-Beatty , D J McGarey , H J Grier , D V Lim . Vibrio harveyi , an opportunistic pathogen of common snook, Centropomus undecimalis (Bloch), held in captivity . Journal of Fish Diseases , 1990 . 13 ( 6 ): 557 - 560 . DOI: 10.1111/j.1365-2761.1990.tb00819.x http://doi.org/10.1111/j.1365-2761.1990.tb00819.x .

H M T Kunttu , E T Valtonen , E I Jokinen , L R Suomalainen . Saprophytism of a fish pathogen as a transmission strategy . Epidemics , 2009 . 1 ( 2 ): 96 - 100 . DOI: 10.1016/j.epidem.2009.04.003 http://doi.org/10.1016/j.epidem.2009.04.003 .

C R Lavilla-Pitogo , M C L Baticados , E R Cruz-Lacierda , L D de la Pena . Occurrence of luminous bacterial disease of Penaeus monodon larvae in the Philippines . Aquaculture , 1990 . 91 ( 1-2 ): 1 - 13 . DOI: 10.1016/0044-8486(90)90173-K http://doi.org/10.1016/0044-8486(90)90173-K .

R Q Li , C Yu , Y R Li , T W Lam , S M Yiu , K Kristiansen , J Wang . SOAP2: an improved ultrafast tool for short read alignment . Bioinformatics , 2009 . 25 ( 15 ): 1 966 - 1 967 . DOI: 10.1093/bioinformatics/btp336 http://doi.org/10.1093/bioinformatics/btp336 .

P C Liu , K K Lee , K C Yii , G H Kou , S N Chen . News & Notes: isolation of Vibrio harveyi from diseased kuruma prawns Penaeus japonicus . Current Microbiology , 1996 . 33 ( 2 ): 129 - 132 . DOI: 10.1007/s002849900087 http://doi.org/10.1007/s002849900087 .

D McDougald , S A Rice , D Weichart , S Kjelleberg . Nonculturability: adaptation or debilitation? . FEMS Microbiology Ecology , 1998 . 25 ( 1 ): 1 - 9 . http://d.old.wanfangdata.com.cn/Periodical/hyxb-e201006010 http://d.old.wanfangdata.com.cn/Periodical/hyxb-e201006010 , .

I Montánchez , I Arana , C Parada , I Garaizabal , M Orruño , I Barcina , V R Kaberdin . Reprogramming of Vibrio harveyi gene expression during adaptation in cold seawater . FEMS Microbiology Ecology , 2014 . 87 ( 1 ): 193 - 203 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=ddaf71ffaafb9a2dd645253007d92401 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=ddaf71ffaafb9a2dd645253007d92401 , .

I Montánchez , E Ogayar , A H Plágaro , A Esteve-Codina , J Gómez-Garrido , M Orruño , I Arana , V R Kaberdin . Analysis of Vibrio harveyi adaptation in sea water microcosms at elevated temperature provides insights into the putative mechanisms of its persistence and spread in the time of global warming . Scientific Reports , 2019 . 9 ( 1 ): 289 DOI: 10.1038/s41598-018-36483-0 http://doi.org/10.1038/s41598-018-36483-0 .

A Mortazavi , B A Williams , K McCue , L Schaeffer , B Wold . Mapping and quantifying mammalian transcriptomes by RNA-Seq . Nature Methods , 2008 . 5 ( 7 ): 621 - 628 . DOI: 10.1038/nmeth.1226 http://doi.org/10.1038/nmeth.1226 .

J A Novitsky , R Y Morita . Morphological characterization of small cells resulting from nutrient starvation of a psychrophilic marine vibrio . Applied and Environmental Microbiolog , 1976 . 32 ( 4 ): 617 - 622 . DOI: 10.1128/AEM.32.4.617-622.1976 http://doi.org/10.1128/AEM.32.4.617-622.1976 .

M Ortigosa , E Garay , M J Pujalte . Numerical taxonomy of Vibrionaceae isolated from oysters and seawater along an annual cycle . Systematic and Applied Microbiology , 1994 . 17 ( 2 ): 216 - 225 . DOI: 10.1016/S0723-2020(11)80011-1 http://doi.org/10.1016/S0723-2020(11)80011-1 .

C Parada , M Orruño , V Kaberdin , Z Bravo , I Barcina , I Arana . Changes in the Vibrio harveyi cell envelope subproteome during permanence in cold seawater . Microbial Ecology , 2016 . 72 ( 3 ): 549 - 558 . DOI: 10.1007/s00248-016-0802-0 http://doi.org/10.1007/s00248-016-0802-0 .

Poindexter J S. 1981. Oligotrophy: fast and famine existence. In : Alexander M ed. Advances in Microbial Ecology. Springer, Boston, MA. p.63-89.

M J Pujalte , A Sitjà-Bobadilla , M C Macián , C Belloch , P Álvarez-Pellitero , J Pérez-Sánchez , F Uruburu , E Garay . Virulence and molecular typing of Vibrio harveyi strains isolated from cultured dentex, gilthead sea bream and European sea bass . Systematic and Applied Microbiology , 2003 . 26 ( 2 ): 284 - 292 . DOI: 10.1078/072320203322346146 http://doi.org/10.1078/072320203322346146 .

M O Saeed . Association of Vibrio harveyi with mortalities in cultured marine fish in Kuwait . Aquaculture , 1995 . 136 ( 1-2 ): 21 - 29 . DOI: 10.1016/0044-8486(95)01045-9 http://doi.org/10.1016/0044-8486(95)01045-9 .

J J Sun , X J Gao , J Qun , X D Du , K R Bi , X J Zhang , L Lin . Comparative analysis of the survival and gene expression of pathogenic strains Vibrio harveyi after starvation . FEMS Microbiology Letters , 2016 . 363 ( 22 ): fnw250 DOI: 10.1093/femsle/fnw250 http://doi.org/10.1093/femsle/fnw250 .

N E Suzina , A L Mulyukin , A N Kozlova , A P Shorokhova , V V Dmitriev , E S Barinova , O N Mokhova , G I El'-Registan , V I Duda . Ultrastructure of resting cells of some non-spore-forming bacteria . Microbiology , 2004 . 73 ( 4 ): 435 - 447 . DOI: 10.1023/B:MICI.0000036990.94039.af http://doi.org/10.1023/B:MICI.0000036990.94039.af .

S L Svensson , M Pryjma , E C Gaynor . Flagella-mediated adhesion and extracellular DNA release contribute to biofilm formation and stress tolerance of Campylobacter jejuni . PLoS One , 2014 . 9 ( 8 ): e106063 DOI: 10.1371/journal.pone.0106063 http://doi.org/10.1371/journal.pone.0106063 .

I N Vatsos , K D Thompson , A Adams . Starvation of Flavobacterium psychrophilum in broth, stream water and distilled water . Diseases of Aquatic Organisms , 2003 . 56 ( 2 ): 115 - 126 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=547bc592687e110863335c6bc686f681 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=547bc592687e110863335c6bc686f681 , .

S N Wai , Y Mizunoe , S I Yoshida . How Vibrio cholerae survive during starvation . FEMS Microbiology Letters , 1999 . 180 ( 2 ): 123 - 131 . DOI: 10.1111/j.1574-6968.1999.tb08786.x http://doi.org/10.1111/j.1574-6968.1999.tb08786.x .

P W Wolf , J D Oliver . Temperature effects on the viable but non-culturable state of Vibrio vulnificus . FEMS Microbiology Letters , 1992 . 101 ( 1 ): 33 - 39 . http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=HighWire000006406489 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=HighWire000006406489 , .

X H Zhang , B Austin . Pathogenicity of Vibrio harveyi to salmonids . Journal of Fish Diseases , 2000 . 23 ( 2 ): 93 - 102 . DOI: 10.1046/j.1365-2761.2000.00214.x http://doi.org/10.1046/j.1365-2761.2000.00214.x .

X J Zhang , X S Bai , B L Yan , K R Bi , L Qin . Vibrio harveyi as a causative agent of mass mortalities of megalopa in the seed production of swimming crab Portunus trituberculatus . Aquaculture International , 2014 . 22 ( 2 ): 661 - 672 . DOI: 10.1007/s10499-013-9695-9 http://doi.org/10.1007/s10499-013-9695-9 .

I Zorrilla , S Arijo , M Chabrillon , P Diaz , E Martinez-Manzanares , M C Balebona , M A Moriñigo . Vibrio species isolated from diseased farmed sole, Solea senegalensis (Kaup), and evaluation of the potential virulence role of their extracellular products . Journal of Fish Diseases , 2003 . 26 ( 2 ): 103 - 108 . DOI: 10.1046/j.1365-2761.2003.00437.x http://doi.org/10.1046/j.1365-2761.2003.00437.x .

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Transcriptional responses to starvation of pathogenic Vibrio harveyi strain DY1

Transcriptional responses to starvation of pathogenic Vibrio harveyi strain DY1

DOI：10.1007/s00343-019-8350-3

摘要

Abstract

关键词

Keywords

references