Cyclic adenosine monophosphate signal pathway is involved in regulation of triacylglycerol biosynthesis following nitrogen deprivation in <italic style="font-style: italic">Chlamydomonas reinhardtii</italic>

MIAO Rongli; HUANG Kaiyao

doi:10.1007/s00343-019-8345-0

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Cyclic adenosine monophosphate signal pathway is involved in regulation of triacylglycerol biosynthesis following nitrogen deprivation in Chlamydomonas reinhardtii

Updated：2023-04-27

- Cyclic adenosine monophosphate signal pathway is involved in regulation of triacylglycerol biosynthesis following nitrogen deprivation in Chlamydomonas reinhardtii
- Cyclic adenosine monophosphate signal pathway is involved in regulation of triacylglycerol biosynthesis following nitrogen deprivation in Chlamydomonas reinhardtii
- 海洋湖沼学报（英文） 2020年38卷第2期页码：517-528
- Affiliations：
  
  1.Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
  2.University of Chinese Academy of Sciences, Beijing 100049, China
- Author bio：
  
  MIAO Rongli, mronli@ihb.ac.cn
- Funds：
  
  the National High Technology Research and Development Program of China (863 Program)(2014AA022001);© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2020
- DOI：10.1007/s00343-019-8345-0
  中图分类号：
- 收稿：2018-11-30，
  
  录用：2019-5-10，
  
  网络首发：2019-07-16，
  
  纸质出版：2020-03
- Accepted：
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Cyclic adenosine monophosphate signal pathway is involved in regulation of triacylglycerol biosynthesis following nitrogen deprivation in Chlamydomonas reinhardtii[J]. 海洋湖沼学报（英文）, 2020,38(2):517-528.

MIAO Rongli, HUANG Kaiyao. Cyclic adenosine monophosphate signal pathway is involved in regulation of triacylglycerol biosynthesis following nitrogen deprivation in Chlamydomonas reinhardtii[J]. Journal of Oceanology and Limnology, 2020, 38(2): 517-528.
Cyclic adenosine monophosphate signal pathway is involved in regulation of triacylglycerol biosynthesis following nitrogen deprivation in Chlamydomonas reinhardtii[J]. 海洋湖沼学报（英文）, 2020,38(2):517-528. DOI： 10.1007/s00343-019-8345-0.

MIAO Rongli, HUANG Kaiyao. Cyclic adenosine monophosphate signal pathway is involved in regulation of triacylglycerol biosynthesis following nitrogen deprivation in Chlamydomonas reinhardtii[J]. Journal of Oceanology and Limnology, 2020, 38(2): 517-528. DOI： 10.1007/s00343-019-8345-0.

摘要

Abstract

The unicellular green alga

Chlamydomonas reinhardtii

is a model organism for studying various biological processes

such as photosynthesis

flagellar motility

and lipid metabolism. To find some novel genes regulating the lipid metabolism under various stress conditions

the paromomycin resistance gene

aphVⅢ

was transferred into the genome of

C. reinhardtii

to establish a mutant library. Two genes mutated in two of the TAG-reduced mutants (Cre06.g278111 in M2 mutant

Cre06.g278110 in M6 mutants) were neighboring in the genome

and their expression levels were down-regulated in their corresponding mutants in parallel with their reduced TAG levels following N deprivation. The proteins encoded by these two genes (KCN11 by Cre06.g278111

ACYC3 by Cre06.g278110) contained a conversed cyclic mononucleotide phosphate (cNMP) binding protein and an adenylate domain

respectively. Since cNMP binding protein and adenylate domain have been known as important components of cyclic adenosine monophosphate (cAMP) signaling pathway

suggesting that these two genes might affect cellular TAG biosynthesis through cAMP signal pathway.

关键词

Keywords

references

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Cyclic adenosine monophosphate signal pathway is involved in regulation of triacylglycerol biosynthesis following nitrogen deprivation in Chlamydomonas reinhardtii

Cyclic adenosine monophosphate signal pathway is involved in regulation of triacylglycerol biosynthesis following nitrogen deprivation in Chlamydomonas reinhardtii

DOI：10.1007/s00343-019-8345-0

摘要

Abstract

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Keywords

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