Characteristics of intracellular polyphosphate granules and phosphorus-absorption of a marine polyphosphateaccumulating bacterium, <italic style="font-style: italic">Halomonas</italic> sp. YSR-3

Shiying REN; Xiangqian LI; Xiulian YIN; Chuping LUO; Fei LIU

doi:10.1007/s00343-019-8323-6

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Characteristics of intracellular polyphosphate granules and phosphorus-absorption of a marine polyphosphateaccumulating bacterium, Halomonas sp. YSR-3

Biology | Updated：2023-04-27

- Characteristics of intracellular polyphosphate granules and phosphorus-absorption of a marine polyphosphateaccumulating bacterium, Halomonas sp. YSR-3
- Characteristics of intracellular polyphosphate granules and phosphorus-absorption of a marine polyphosphateaccumulating bacterium, Halomonas sp. YSR-3
- 海洋湖沼学报（英文） 2020年38卷第1期页码：195-203
- Affiliations：
  
  1.Jiangsu Provincial Key Construction Laboratory of Probiotics Preparation, Huaiyin Institute of Technology, Huai'an 223003, China
  2.Jiangsu Provincial Engineering Laboratory for Advanced Materials of Salt Chemical Industry, Huaiyin Institute of Technology, Huai'an 223003, China
  3.School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai'an 223003, China
- Author bio：
  
  LUO Chuping, luochuping@163.com
  LIU Fei, hyitliu@163.com
- Funds：
  
  the National Natural Science Foundation of China(51308245);the National Natural Science Foundation of China(31570061);the Open Project of Jiangsu Provincial Engineering Laboratory for Advanced Materials of Salt Chemical Industry(SF201408);the Open Project of Jiangsu Provincial Key Construction Laboratory of Probiotics Preparation(JSYSZJ2017006);© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2020
- DOI：10.1007/s00343-019-8323-6
  中图分类号：
- 收稿：2018-11-06，
  
  录用：2018-12-24，
  
  网络首发：2019-03-08，
  
  纸质出版：2020-01
- Accepted：
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Characteristics of intracellular polyphosphate granules and phosphorus-absorption of a marine polyphosphateaccumulating bacterium, Halomonas sp. YSR-3[J]. 海洋湖沼学报（英文）, 2020,38(1):195-203.

Shiying REN, Xiangqian LI, Xiulian YIN, et al. Characteristics of intracellular polyphosphate granules and phosphorus-absorption of a marine polyphosphateaccumulating bacterium, Halomonas sp. YSR-3[J]. Journal of Oceanology and Limnology, 2020, 38(1): 195-203.
Characteristics of intracellular polyphosphate granules and phosphorus-absorption of a marine polyphosphateaccumulating bacterium, Halomonas sp. YSR-3[J]. 海洋湖沼学报（英文）, 2020,38(1):195-203. DOI： 10.1007/s00343-019-8323-6.

Shiying REN, Xiangqian LI, Xiulian YIN, et al. Characteristics of intracellular polyphosphate granules and phosphorus-absorption of a marine polyphosphateaccumulating bacterium, Halomonas sp. YSR-3[J]. Journal of Oceanology and Limnology, 2020, 38(1): 195-203. DOI： 10.1007/s00343-019-8323-6.

摘要

Abstract

Halomonas

sp. YSR-3 was isolated from the Yellow Sea and identified as a polyphosphateaccumulating bacterium and the characteristics of its intracellular polyphosphate (polyP) granules and phosphorus absorption were studied. Most YSR-3 cells stored one or two polyP granules in regular appearance and high-density. The diameter of the granules was about 400 nm measuring by a transmission electron microscope (TEM). After stained with 4

6-diamidino-2-phenylindole (DAPI) and visualized by a fluorescence microscope

the cells turned blue and the granules were bright yellow. The composition of granules includes P (major ingredient)

and Ca as detected by an energy dispersive X-ray spectrometer (EDS). When inorganic phosphorus (PO

) and ferric ion (Fe

) were added into media

the biomass increased and the cells formed intracellular polyP granules owing to the phosphorus assimilation from media. The YSR-3 obtained higher biomass by adding 0.02 g/L FePO

than 0.005 g/L and 0.01 g/L FePO

; however

the phosphorus absorption was higher with 0.01 g/L FePO

than 0.005 g/L and 0.02 g/L FePO

. The optical density at wavelength 480 nm (OD

480 nm

) was 0.79 and 100% cells could form intracellular polyP granules. These results show that strain YSR-3 is able to acquire higher biomass and absorb more inorganic phosphorus when 0.01 g/L FePO

is added. The characteristics of absorbing and storing phosphorus as intracellular inorganic polyP granules have a potential for application in high-efficiency phosphorus removal in wastewater treatment.

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Characteristics of intracellular polyphosphate granules and phosphorus-absorption of a marine polyphosphateaccumulating bacterium, Halomonas sp. YSR-3

Characteristics of intracellular polyphosphate granules and phosphorus-absorption of a marine polyphosphateaccumulating bacterium, Halomonas sp. YSR-3

DOI：10.1007/s00343-019-8323-6

摘要

Abstract

关键词

Keywords

references