Isolation, characterization and expression analysis of <italic style="font-style: italic">TRPV4</italic> in half-smooth tongue sole <italic style="font-style: italic">Cynoglossus semilaevis</italic>

Xiaomei SHANG; Aijun MA; Xin'an WANG; Dandan XIA; Jiao ZHUANG

doi:10.1007/s00343-019-8316-5

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Isolation, characterization and expression analysis of TRPV4 in half-smooth tongue sole Cynoglossus semilaevis

Aquaculture and Fisheries | Updated：2023-04-27

- Isolation, characterization and expression analysis of TRPV4 in half-smooth tongue sole Cynoglossus semilaevis
- Isolation, characterization and expression analysis of TRPV4 in half-smooth tongue sole Cynoglossus semilaevis
- 海洋湖沼学报（英文） 2020年38卷第1期页码：294-305
- Affiliations：
  
  1.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences; Shandong Key Laboratory of Marine Fisheries Biotechnology and Genetic Breeding; Qingdao Key Laboratory for Marine Fish Breeding and Biotechnology, Qingdao 266071, China
  2.Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266237, China
  3.College of Marine and Environmental Sciences, Tianjin University of Science and Technology, Tianjin 300457, China
- Author bio：
  
  MA Aijun, maaj@ysfri.ac.cn
- Funds：
  
  the Earmarked Fund for Modern Agro-Industry Technology Research System(CARS-47-G01);the AoShan Talents Cultivation Program supported by Qingdao National Laboratory for Marine Science and Technology(2017ASTCP-OS04);the Qingdao Natural Science Foundation(12-1-4-12-(1)-jch);© Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag GmbH Germany, part of Springer Nature 2020
- DOI：10.1007/s00343-019-8316-5
  中图分类号：
- 收稿：2018-11-06，
  
  录用：2019-2-26，
  
  网络首发：2019-03-22，
  
  纸质出版：2020-01
- Accepted：
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Isolation, characterization and expression analysis of TRPV4 in half-smooth tongue sole Cynoglossus semilaevis[J]. 海洋湖沼学报（英文）, 2020,38(1):294-305.

Xiaomei SHANG, Aijun MA, Xin'an WANG, et al. Isolation, characterization and expression analysis of TRPV4 in half-smooth tongue sole Cynoglossus semilaevis[J]. Journal of Oceanology and Limnology, 2020, 38(1): 294-305.
Isolation, characterization and expression analysis of TRPV4 in half-smooth tongue sole Cynoglossus semilaevis[J]. 海洋湖沼学报（英文）, 2020,38(1):294-305. DOI： 10.1007/s00343-019-8316-5.

Xiaomei SHANG, Aijun MA, Xin'an WANG, et al. Isolation, characterization and expression analysis of TRPV4 in half-smooth tongue sole Cynoglossus semilaevis[J]. Journal of Oceanology and Limnology, 2020, 38(1): 294-305. DOI： 10.1007/s00343-019-8316-5.

摘要

Abstract

The transient receptor potential vanilloid 4 (TRPV4)

another Ca

entry channel

belongs to the vanilloid subfamily and responds to a number of different physical and chemical stimuli and exists widely in mammals. However

our understanding of the TRPV4 in fish remains poor. Therefore

we studied the

TRPV4

gene from

Cynoglossus semilaevis

named

CsTRPV4

that encodes a putative protein of 870 amino acids common in structure and characteristic of mammalian TRPV4

including the domains of ANK repeats

six TM

TRP domain

and CaMBD. The

CsTRPV4

was expressed ubiquitously in examined tissues:higher expression in the heart

spleen

testis

and eye

but lower expression in kidney and liver. Surprisingly

the expression of

CsTRPV4

in lateral line was significantly higher than in many other tissues as the

CsTRPV4

was expressed significantly in the free neuromasts. In addition

CsTRPV4

in the free neuromast from the larval fish was significantly expressed in the hair cells of the free neuromasts. Therefore

the free neuromasts can act as a mechano-sensor to the mechanical stimulation in molecular level in

C. semilaevis

which lays a foundation for further study of the functions of the free neuromasts.

关键词

Keywords

references

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Isolation, characterization and expression analysis of TRPV4 in half-smooth tongue sole Cynoglossus semilaevis

DOI：10.1007/s00343-019-8316-5

摘要

Abstract

关键词

Keywords

references