iTRAQ-based analysis of 17β-estradiol induced proteome in Chinese tongue sole <italic style="font-style: italic">Cynoglossus semilaevis</italic>

Ying ZHU; Yangzhen LI; Hailong LI; Lei WANG; Ning ZHANG; Yang LIU; Liang MENG; Xiwen XU; Zhongdian DONG; Min WEI; Hua GUO; Zhongkai CUI; Xihong LI; Changwei SHAO; Wenteng XU

doi:10.1007/s00343-019-8222-x

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iTRAQ-based analysis of 17β-estradiol induced proteome in Chinese tongue sole Cynoglossus semilaevis

Biology | Updated：2023-05-20

- iTRAQ-based analysis of 17β-estradiol induced proteome in Chinese tongue sole Cynoglossus semilaevis
- iTRAQ-based analysis of 17β-estradiol induced proteome in Chinese tongue sole Cynoglossus semilaevis
- 海洋湖沼学报（英文） 2019年37卷第5期页码：1659-1668
- Affiliations：
  
  1.Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China
  2.Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China
  3.Research Institute of Metabolic Disease, Qingdao University, Qingdao 266003, China
- Author bio：
  
  XU Wenteng, xuwt@ysfri.ac.cn
- Funds：
  
  the Natural Science Foundation of Shandong Province(ZR2014CQ053);the National Natural Science Foundation of China(31402293);the Taishan Scholar Climbing Program of Shandong Province, China
- DOI：10.1007/s00343-019-8222-x
  中图分类号：
- 收稿：2018-08-31，
  
  录用：2018-11-21，
  
  网络首发：2019-01-07，
  
  纸质出版：2019-09
- Accepted：
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iTRAQ-based analysis of 17β-estradiol induced proteome in Chinese tongue sole Cynoglossus semilaevis[J]. 海洋湖沼学报（英文）, 2019,37(5):1659-1668.

Ying ZHU, Yangzhen LI, Hailong LI, et al. iTRAQ-based analysis of 17β-estradiol induced proteome in Chinese tongue sole Cynoglossus semilaevis[J]. Journal of Oceanology and Limnology, 2019, 37(5): 1659-1668.
iTRAQ-based analysis of 17β-estradiol induced proteome in Chinese tongue sole Cynoglossus semilaevis[J]. 海洋湖沼学报（英文）, 2019,37(5):1659-1668. DOI： 10.1007/s00343-019-8222-x.

Ying ZHU, Yangzhen LI, Hailong LI, et al. iTRAQ-based analysis of 17β-estradiol induced proteome in Chinese tongue sole Cynoglossus semilaevis[J]. Journal of Oceanology and Limnology, 2019, 37(5): 1659-1668. DOI： 10.1007/s00343-019-8222-x.

摘要

Abstract

The phenomenon of sex dimorphism prevails among many fish species. It has attracted the general research interest due to its close relationship to fish growth and thus aquaculture productivity. In Chinese tongue sole (

Cynoglossus semilaevis

)

17β-estradiol (E2) can be used reportedly to induce the feminization of fish

although the detailed regulatory network remained elusive. We treated the tongue sole fry before sex differentiation with E2 (10 and 30 μg/L) to identify potential targets of E2 in Chinese tongue sole. The E2 treatment indeed induced genetic male fish sex-reversal to phenotypic female. Using an iTRAQ-based comparative proteomic analysis

409 proteins that differentially expressed after E2 induction were identified

including 259 up-regulated and 150 down-regulated proteins. Furthermore

19 differentially expressed proteins identified in the comparative proteomic analysis were selected to assess their transcription and eight of them exhibited the same tendency. Functions of the eight proteins included mainly nucleotide and protein binding. Interestingly

a far upstream element-binding protein 3-like isoform exhibited the significant upregulation both at transcription and translation levels after E2 treatment. This work identified a set of candidate proteins for E2 response and deepened our understanding of E2 regulatory mechanism.

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DOI：10.1007/s00343-019-8222-x

摘要

Abstract

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