Down-regulation of <italic style="font-style: italic">SEIPIN</italic> transcription attenuated the triacylglycerol accumulation in <italic style="font-style: italic">Nannochloropsis</italic> <italic style="font-style: italic">oceanica</italic>

Lixian LIU; Li GUO; Hang LIU; Xiaohui PAN; Yanan ZONG; Leili FENG; Guanpin YANG

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Down-regulation of SEIPIN transcription attenuated the triacylglycerol accumulation in Nannochloropsis oceanica

Biology | Updated：2025-02-19

- Down-regulation of SEIPIN transcription attenuated the triacylglycerol accumulation in Nannochloropsis oceanica
- Down-regulation of SEIPIN transcription attenuated the triacylglycerol accumulation in Nannochloropsis oceanica
- 海洋湖沼学报(英文) 2025年43卷第1期页码：187-195
- Affiliations：
  
  1.College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
  2.Institutes of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China
  3.Key Laboratory of Marine Genetics and Breeding of Ministry of Education, Ocean University of China, Qingdao 266003, China
  4.MoE Laboratory of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China
- Author bio：
  
  qdguoli@139.com
  yguanpin@ouc.edu.cn
- Funds：
  
  the National Key R&D Program of China(2022YFF1102300);the Key R&D Program of Shandong Province, China(2022LZGC004);the National Natural Science Foundation of China(32102757);the Science & Technology Innovation Project of Laoshan Laboratory(LSKJ202203205);the Fundamental Research Funds for the Central Universities(202262001)
- DOI：
  中图分类号：
- 收稿：2023-12-24，
  
  纸质出版：2025-01-01
- Accepted：
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Down-regulation of SEIPIN transcription attenuated the triacylglycerol accumulation in Nannochloropsis oceanica[J]. 海洋湖沼学报(英文), 2025,43(1):187-195.

LIU Lixian,GUO Li,LIU Hang,et al.Down-regulation of SEIPIN transcription attenuated the triacylglycerol accumulation in Nannochloropsis oceanica[J].Journal of Oceanology and Limnology,2025,43(01):187-195.
Down-regulation of SEIPIN transcription attenuated the triacylglycerol accumulation in Nannochloropsis oceanica[J]. 海洋湖沼学报(英文), 2025,43(1):187-195. DOI：

LIU Lixian,GUO Li,LIU Hang,et al.Down-regulation of SEIPIN transcription attenuated the triacylglycerol accumulation in Nannochloropsis oceanica[J].Journal of Oceanology and Limnology,2025,43(01):187-195. DOI：

摘要

Abstract

Abnormal content of protein SEIPIN causes Berardinelli-Seip congenital lipodystrophy type 2 (BSCL2) in humans. Previous studies have shown that

Arabidopsis

thaliana

has evolved three SEIPIN homologs

determining the fusion and size of lipid droplets.

Nannochloropsis

oceanica

is a model oleaginous microalga with the accumulation potential of fatty acids. We believe that the SEIPIN homolog if any in

oceanica

should function as it does in humans

plant among others. In this study

we identified a homologous gene of

SEIPIN

oceanica

constructed a bacterial plasmid containing the elements controlling the transcription of its complete antisense RNA

and integrated the linearized plasmid into the

oceanica

genome. We obtained a transformant in which the abundance of

SEIPIN

transcript is significantly down-regulated. Flow cytometry analysis showed that the triacylglycerol (TAG) content of the transformant is significantly lower than that of the wild type (WT). Transcriptomic analysis showed that the down-regulation of

SEIPIN

transcript abundance could affect the protein functioning in endoplasmic reticulum (ER)

implying that

SEIPIN

gene can function in

oceanica

in a similar way to that in

thaliana

关键词

Keywords

references

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Down-regulation of SEIPIN transcription attenuated the triacylglycerol accumulation in Nannochloropsis oceanica

Down-regulation of SEIPIN transcription attenuated the triacylglycerol accumulation in Nannochloropsis oceanica

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摘要

Abstract

关键词

Keywords

references