The effect of typical seawater cations on the adhesion of mussel protein Mefp-1 based on dissipative quartz crystal microbalance

Futao ZHANG; Chengjun SUN; Linqing XIE; Fuqian LI; Fenghua JIANG; Jingxi LI

Your Location：

Home >

Browse articles >

The effect of typical seawater cations on the adhesion of mussel protein Mefp-1 based on dissipative quartz crystal microbalance

Biology | Updated：2025-02-19

- The effect of typical seawater cations on the adhesion of mussel protein Mefp-1 based on dissipative quartz crystal microbalance
- The effect of typical seawater cations on the adhesion of mussel protein Mefp-1 based on dissipative quartz crystal microbalance
- 海洋湖沼学报(英文) 2025年43卷第1期页码：274-285
- Affiliations：
  
  1.Marine Bioresource and Environment Research Center, First Institute of Oceanography, Ministry of Natural Resources, Qingdao 266061, China
  2.Laboratory of Marine Drugs and Bioproducts, Laoshan Laboratory, Qingdao 266237, China
  3.Shandong Jinpiao Food Machinery Co., Ltd., Weihai 264300, China
- Author bio：
  
  csun@fio.org.cn
- Funds：
  
  the National Natural Science Foundation of China(41776177);the Qingdao Marine Science and Technology Pilot National Laboratory Fund(2016ASKJ14;QNLM2016ORP0403)
- DOI：
  中图分类号：
- 收稿：2024-02-04，
  
  纸质出版：2025-01-01
- Accepted：
Scan QR Code
The effect of typical seawater cations on the adhesion of mussel protein Mefp-1 based on dissipative quartz crystal microbalance[J]. 海洋湖沼学报(英文), 2025,43(1):274-285.

ZHANG Futao,SUN Chengjun,XIE Linqing,et al.The effect of typical seawater cations on the adhesion of mussel protein Mefp-1 based on dissipative quartz crystal microbalance[J].Journal of Oceanology and Limnology,2025,43(01):274-285.
The effect of typical seawater cations on the adhesion of mussel protein Mefp-1 based on dissipative quartz crystal microbalance[J]. 海洋湖沼学报(英文), 2025,43(1):274-285. DOI：

ZHANG Futao,SUN Chengjun,XIE Linqing,et al.The effect of typical seawater cations on the adhesion of mussel protein Mefp-1 based on dissipative quartz crystal microbalance[J].Journal of Oceanology and Limnology,2025,43(01):274-285. DOI：

摘要

Abstract

Mussels are common anchoring organisms that adhere to the surfaces of various substrates with their byssus. The adhesion of mussel to substrates is contingent upon the presence of mussel foot proteins

of which

Mytilus

edulis

foot protein-1 (Mefp-1) has been identified as the most abundant protein. It has been found that lipids are involved in the mussel adhesion process and can facilitate Mefp-1 adhesion. In this research

the adhesion behavior of Mefp-1 on various substrate surfaces under the effect of typical seawater cations with or without the presence of lipid were investigated using a quartz crystal microbalance with dissipation (QCM-D). Results indicate that the presence of cations Ca

and K

leads to varying degrees of reduction in the adhesion performance of Mefp-1 on different substrates. The degree of this reduction

however

was much alleviated in the presence of palmitic acid

which is involved in the mussel adhesion process. Therefore

the involvement of palmitic acid is advantageous for mussel protein adhesion to the substrate surface in the marine environment. This study illustrated the significant contribution of palmitic acid to mussel adhesion

which can help to better understand biofouling mechanisms and develop biomimetic adhesive materials.

关键词

Keywords

references

Ahn B K . 2017 . Perspectives on mussel-inspired wet adhesion . Journal of the American Chemical Society , 139 ( 30 ): 10166 - 10171 , https://doi.org/10.1021/jacs.6b13149 https://doi.org/10.1021/jacs.6b13149 .

An W Z , Liu Q , Fu W L et al . 2021 . Research progress in mussel adhesion proteins . Military Medical Sciences , 45 ( 10 ): 798 - 800 , https://doi.‍org/10.7644/j.issn.‍1674-9960.2021.10.016. https://doi.‍org/10.7644/j.issn.‍1674-9960.2021.10.016. (in Chinese with English abstract)

Ausili A , Berglin M , Elwing H et al . 2012 . Quartz crystal microbalance with dissipation monitoring and the real-time study of biological systems and macromolecules at interfaces . Biomedical Spectroscopy and Imaging , 1 ( 4 ): 325 - 338 , https://doi.org/10.3233/bsi-2012-0025 https://doi.org/10.3233/bsi-2012-0025 .

Chen T , Yang H , Gao H W et al . 2017 . Adsorption and orientation of 3,4-dihydroxy-l-phenylalanine onto tunable monolayer films . The Journal of Physical Chemistry C , 121 ( 21 ): 11544 - 11551 , https://doi.org/10.1021/acs.jpcc.7b02795 https://doi.org/10.1021/acs.jpcc.7b02795 .

Danner E W , Kan Y J , Hammer M U et al . 2012 . Adhesion of mussel foot protein mefp-5 to mica: an underwater superglue . Biochemistry , 51 ( 33 ): 6511 - 6518 , https://doi.org/10.1021/bi3002538 https://doi.org/10.1021/bi3002538 .

Deacon M P , Davis S S , Waite J H et al . 1998 . Structure and mucoadhesion of mussel glue protein in dilute solution . Biochemistry , 37 ( 40 ): 14108 - 14112 , https://doi.org/10.1021/bi9806192 https://doi.org/10.1021/bi9806192 .

Debabhuti N , Mukherjee S , Neogi S et al . 2022 . A study of vegetable oil modified QCM sensor to detect β-pinene in Indian cardamom . Talanta , 236 : 122837 , https://doi.org/10.1016/j.talanta.2021.122837 https://doi.org/10.1016/j.talanta.2021.122837 .

Fan H L , Wang J H , Tao Z et al . 2019 . Adjacent cationic-aromatic sequences yield strong electrostatic adhesion of hydrogels in seawater . Nature Communications , 10 ( 1 ): 5127 , https://doi.org/10.1038/s41467-019-13171-9 https://doi.org/10.1038/s41467-019-13171-9 .

Feiler A A , Sahlholm A , Sandberg T et al . 2007 . Adsorption and viscoelastic properties of fractionated mucin (BSM) and bovine serum albumin (BSA) studied with quartz crystal microbalance (QCM-D) . Journal of Colloid and Interface Science , 315 ( 2 ): 475 - 481 , https://doi.org/10.1016/j.jcis.2007.07.029 https://doi.org/10.1016/j.jcis.2007.07.029 .

Gao M , Zhang C H , Zhou J et al . 2011 . Review of mussel adhesive protein . Journal of Anhui Agricultural Sciences , 39 ( 32 ): 19860 - 19862 , https://doi.org/10.13989/j.cnki.0517-6611.2011.32.242. https://doi.org/10.13989/j.cnki.0517-6611.2011.32.242. (in Chinese)

Gebbie M A , Wei W , Schrader A M et al . 2017 . Tuning underwater adhesion with cation-π interactions . Nature Chemistry , 9 ( 5 ): 473 - 479 , https://doi.org/10.1038/nchem.2720 https://doi.org/10.1038/nchem.2720 .

He Y H , Sun C J , Jiang F H et al . 2018 . Lipids as integral components in mussel adhesion . Soft Matter , 14 ( 35 ): 7145 - 7154 , https://doi.org/10.1039/c8sm00509e https://doi.org/10.1039/c8sm00509e .

Hennebicq R , Fabra G , Pellerin C et al . 2013 . The effect of spawning of cultured mussels ( Mytilus edulis ) on mechanical properties, chemical and biochemical composition of byssal threads . Aquaculture , 410 - 411 : 11 - 17 , https://doi.org/10.1016/j.aquaculture.2013.06.011 https://doi.org/10.1016/j.aquaculture.2013.06.011 .

Höök F , Kasemo B , Nylander T et al . 2001 . Variations in coupled water, viscoelastic properties, and film thickness of a Mefp-1 protein film during adsorption and cross-linking: a quartz crystal microbalance with dissipation monitoring, ellipsometry, and surface plasmon resonance study . Analytical Chemistry , 73 ( 24 ): 5796 - 5804 , https://doi.org/10.1021/ac0106501 https://doi.org/10.1021/ac0106501 .

Inoue K , Takeuchi Y , Miki D et al . 1995 . Mussel adhesive plaque protein gene is a novel member of epidermal growth factor-like gene family . Journal of Biological Chemistry , 270 ( 12 ): 6698 - 6701 , https://doi.org/10.1074/jbc.270.12.6698 https://doi.org/10.1074/jbc.270.12.6698 .

Israelachvili J N . 2011 . Intermolecular and Surface Forces. 3 rd edn . Academic Press, Burlington . p. 151 - 167 . https://do 10.1016/b978-0-12-391927-4.10008-8 http://dx.doi.org/10.1016/b978-0-12-391927-4.10008-8

Johnson S B , Franks G V , Scales P J et al . 2000 . Surface chemistry-rheology relationships in concentrated mineral suspensions . International Journal of Mineral Processing , 58 ( 1 ): 267 - 304 , https://doi.org/10.1016/S0301-7516(99)00041-1 https://doi.org/10.1016/S0301-7516(99)00041-1 .

Krivosheeva O , Dėdinaitė A , Claesson P M . 2012 . Adsorption of Mefp - 1 : influence of pH on adsorption kinetics and adsorbed amount . Journal of Colloid and Interface Science , 379 ( 1 ): 107 - 113 , https://doi.org/10.1016/j.jcis.2012.04.040 https://doi.org/10.1016/j.jcis.2012.04.040 .

Lin Q , Gourdon D , Sun C J et al . 2007 . Adhesion mechanisms of the mussel foot proteins mfp-1 and mfp-3 . Proceedings of the National Academy of Sciences of the United States of America , 104 ( 10 ): 3782 - 3786 , https://doi.org/10.1073/pnas.0607852104 https://doi.org/10.1073/pnas.0607852104 .

Lundin M , Elofsson U M , Blomberg E et al . 2010 . Adsorption of lysozyme, β-casein and their layer-by-layer formation on hydrophilic surfaces: effect of ionic strength . Colloids and Surfaces B: Biointerfaces , 77 ( 1 ): 1 - 11 , https://doi.org/10.1016/j.colsurfb.2009.12.019 https://doi.org/10.1016/j.colsurfb.2009.12.019 .

Mesko M , Xiang L , Bohle S et al . 2021 . Catechol-vanadium binding enhances cross-linking and mechanics of a mussel byssus coating protein . Chemistry of Materials , 33 ( 16 ): 6530 - 6540 , https://doi.org/10.1021/acs.chemmater.1c02063 https://doi.org/10.1021/acs.chemmater.1c02063 .

Ou X W , Xue B , Lao Y C et al . 2020 . Structure and sequence features of mussel adhesive protein lead to its salt-tolerant adhesion ability . Science Advances , 6 ( 39 ): eabb 7620 , https://doi.org/10.1126/sciadv.abb7620 https://doi.org/10.1126/sciadv.abb7620 .

Priemel T , Palia G , Förste F et al . 2021 . Microfluidic-like fabrication of metal ion-cured bioadhesives by mussels . Science , 374 ( 6564 ): 206 - 211 , https://doi.org/10.1126/science.abi9702 https://doi.org/10.1126/science.abi9702 .

Priemel T , Palia R , Babych M et al . 2020 . Compartmentalized processing of catechols during mussel byssus fabrication determines the destiny of DOPA . Proceedings of the National Academy of Sciences of the United States of America , 117 ( 14 ): 7613 - 7621 , https://doi.org/10.1073/pnas.1919712117 https://doi.org/10.1073/pnas.1919712117 .

Rodahl M , Kasemo B . 1996a . On the measurement of thin liquid overlayers with the quartz-crystal microbalance . Sensors and Actuators A: Physical , 54 ( 1-3 ): 448 - 456 , https://doi.org/10.1016/s0924-4247(97)80002-7 https://doi.org/10.1016/s0924-4247(97)80002-7 .

Rodahl M , Kasemo B . 1996b . A simple setup to simultaneously measure the resonant frequency and the absolute dissipation factor of a quartz crystal microbalance . Review of Scientific Instruments , 67 ( 9 ): 3238 - 3241 , https://doi.org/10.1063/1.1147494 https://doi.org/10.1063/1.1147494 .

Schmitt C N Z , Winter A , Bertinetti L et al . 2015 . Mechanical homeostasis of a DOPA-enriched biological coating from mussels in response to metal variation . Journal of the Royal Society Interface , 12 ( 110 ): 20150466 , https://doi.org/10.1098/rsif.2015.0466 https://doi.org/10.1098/rsif.2015.0466 .

Sever M J , Weisser J T , Monahan J et al . 2004 . Metal-mediated cross-linking in the generation of a marine-mussel adhesive . Angewandte Chemie International Edition , 43 ( 4 ): 448 - 450 , https://doi.org/10.1002/anie.200352759 https://doi.org/10.1002/anie.200352759 .

Sun C J , Waite J H . 2005 . Mapping chemical gradients within and along a fibrous structural tissue, mussel byssal threads . Journal of Biological Chemistry , 280 ( 47 ): 39332 - 39336 , https://doi.org/10.1074/jbc.M508674200 https://doi.org/10.1074/jbc.M508674200 .

Sundh M , Svedhem S , Sutherland D S . 2011 . Formation of supported lipid bilayers at surfaces with controlled curvatures: influence of lipid charge . The Journal of Physical Chemistry B , 115 ( 24 ): 7838 - 7848 , https://doi.org/10.1021/jp2025363 https://doi.org/10.1021/jp2025363 .

Turetta N , Sedona F , Liscio A et al . 2021 . Au(111) surface contamination in ambient conditions: unravelling the dynamics of the work function in air . Advanced Materials Interfaces , 8 ( 10 ): 2100068 , https://doi.org/10.1002/admi.202100068 https://doi.org/10.1002/admi.202100068 .

von Cosel R , Olu K . 1998 . Gigantism in Mytilidae. A new Bathymodiolus from cold seep areas on the Barbados accretionary prism . Comptes Rendus de l'Académie des Sciences-Series III-Sciences de la Vie , 321 ( 8 ): 655 - 663 , https://doi.org/10.1016/s0764-4469(98)80005-x https://doi.org/10.1016/s0764-4469(98)80005-x .

Waite J H . 1992 . The DOPA ephemera: a recurrent motif in invertebrates . The Biological Bulletin , 183 ( 1 ): 178 - 184 , https://doi.org/10.2307/1542421 https://doi.org/10.2307/1542421 .

Waite J H . 2017 . Mussel adhesion—essential footwork . Journal of Experimental Biology , 220 ( 4 ): 517 - 530 , https://doi.org/10.1242/jeb.134056 https://doi.org/10.1242/jeb.134056 .

Waite J H , Andersen N H , Jewhurst S et al . 2005 . Mussel adhesion: finding the tricks worth mimicking . The Journal of Adhesion , 81 ( 3-4 ): 297 - 317 , https://doi.org/10.1080/00218460590944602 https://doi.org/10.1080/00218460590944602 .

Waite J H , Qin X X . 2001 . Polyphosphoprotein from the adhesive pads of Mytilus edulis . Biochemistry , 40 ( 9 ): 2887 - 2893 , https://doi.org/10.1021/bi002718x https://doi.org/10.1021/bi002718x .

Waite J H . 1999 . Reverse engineering of bioadhesion in marine mussels . Annals of the New York Academy of Sciences , 875 ( 1 ): 301 - 309 , https://doi.org/10.1111/j.1749-6632.1999.tb08513.x https://doi.org/10.1111/j.1749-6632.1999.tb08513.x .

Wang W N , Xu Y S , Backes S et al . 2016 . Construction of compact polyelectrolyte multilayers inspired by marine mussel: effects of salt concentration and pH as observed by QCM-D and AFM . Langmuir , 32 ( 14 ): 3365 - 3374 , https://doi.org/10.1021/acs.langmuir.5b04706 https://doi.org/10.1021/acs.langmuir.5b04706 .

Weinhold M , Soubatch S , Temirov R et al . 2006 . Structure and bonding of the multifunctional amino acid L-DOPA on Au ( 110 ). The Journal of Physical Chemistry B , 110 ( 47 ): 23756 - 23769 , https://doi.org/10.1021/jp064956t https://doi.org/10.1021/jp064956t .

Xie L Q , Cao W , Sun C J . 2023 . The study of fatty acid mediated Mefp-1 adsorption by Quartz Crystal Microbalance with Dissipation . The Journal of Adhesion , 99 ( 8 ): 1263 - 1281 , https://doi.‍org/10.1080/00218464.2022.2099740 https://doi.‍org/10.1080/00218464.2022.2099740 . https://do 10.1080/00218464.2022.2099740 http://dx.doi.org/10.1080/00218464.2022.2099740

Xu Y W , Ji Y L , Ma J H . 2021 . Hydrophobic and hydrophilic effects in a mussel-inspired citrate-based adhesive . Langmuir , 37 ( 1 ): 311 - 321 , https://doi.org/10.1021/acs.langmuir.0c02895 https://doi.org/10.1021/acs.langmuir.0c02895 .

Yaminsky V V , Ninham B W , Pashley R M . 1998 . Interaction between surfaces of fused silica in water. evidence of cold fusion and effects of cold plasma treatment . Langmuir , 14 ( 12 ): 3223 - 3235 , https://doi.org/10.1021/la9713762 https://doi.org/10.1021/la9713762 .

Zhang W , Yang H , Liu F H et al . 2017 . Molecular interactions between DOPA and surfaces with different functional groups: a chemical force microscopy study . RSC Advances , 7 ( 52 ): 32518 - 32527 , https://doi.org/10.1039/c7ra04228k https://doi.org/10.1039/c7ra04228k .

Zhao H , Waite J H . 2006a . Linking adhesive and structural proteins in the attachment plaque of Mytilus californianus . Journal of Biological Chemistry , 281 ( 36 ): 26150 - 26158 , https://doi.‍org/10.1074/jbc.M604357200 https://doi.‍org/10.1074/jbc.M604357200 .

Zhao H , Waite J H . 2006b . Proteins in load-bearing junctions: the histidine-rich metal-binding protein of mussel byssus . Biochemistry , 45 ( 47 ): 14223 - 14231 , https://doi.org/10.1021/bi061677n https://doi.org/10.1021/bi061677n .

Zhou Y , Kang L Z , Yue Z L et al . 2020 . Composite tissue adhesive containing catechol-modified hyaluronic acid and poly-L-lysine . ACS Applied Bio Materials , 3 ( 1 ): 628 - 638 , https://doi.org/10.1021/acsabm.9b01003 https://doi.org/10.1021/acsabm.9b01003 .

Zhu Y Y , Ding H B , Sun C J . 2014 . The research progress on mussel adhesive proteins . Advances in Marine Science , 32 ( 4 ): 560 - 570 , https://doi.org/10.3969/j.issn.1671-6647.2014.04.014. https://doi.org/10.3969/j.issn.1671-6647.2014.04.014. (in Chinese with English abstract)

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

暂无数据