RNA interference reveals chloride channel 7 gene helps short-term hypersalinity stress resistance in Hong Kong oyster <italic style="font-style: italic">Crassostrea</italic> <italic style="font-style: italic">hongkongensis</italic>

Yeshao PENG; Ziao CHEN; Qiong DENG; Zhen JIA; Lingxin KONG; Peng ZHU; Youhou XU; Zhicai SHE

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RNA interference reveals chloride channel 7 gene helps short-term hypersalinity stress resistance in Hong Kong oyster Crassostrea hongkongensis

Biology | Updated：2024-10-11

- RNA interference reveals chloride channel 7 gene helps short-term hypersalinity stress resistance in Hong Kong oyster Crassostrea hongkongensis
- RNA interference reveals chloride channel 7 gene helps short-term hypersalinity stress resistance in Hong Kong oyster Crassostrea hongkongensis
- 海洋湖沼学报(英文) 2024年42卷第4期页码：1261-1271
- Affiliations：
  
  Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Pinglu Canal and Beibu Gulf Coastal Ecosystem Observation and Research Station of Guangxi, College of Marine Sciences, Beibu Gulf University, Qinzhou 535011, China
- Author bio：
  
  xuxuyoutiao@163.com
  shezc@bbgu.edu.cn
- Funds：
  
  the Natural Science Foundation of Guangxi Province(2023GXNSFAA026503;2018GXNSFBA281201);the Guangxi Key Research and Development Program(GuikeAB21196030);the Marine Science Guangxi First-Class Subject, Beibu Gulf University(DRC002);the Scientific Research and Technology Development Plan Project of Qinzhou(202014842;20223637);the Science and Technology Major Project of Guangxi Province(AA17204095-10);the Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Beibu Gulf University(2020ZB09;2020ZB04)
- DOI：
  中图分类号：
- 收稿：2023-07-15，
  
  纸质出版：2024-07-01
- Accepted：
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RNA interference reveals chloride channel 7 gene helps short-term hypersalinity stress resistance in Hong Kong oyster Crassostrea hongkongensis[J]. 海洋湖沼学报(英文), 2024,42(4):1261-1271.

PENG Yeshao,CHEN Ziao,DENG Qiong,et al.RNA interference reveals chloride channel 7 gene helps short-term hypersalinity stress resistance in Hong Kong oyster Crassostreahongkongensis[J].Journal of Oceanology and Limnology,2024,42(04):1261-1271.
RNA interference reveals chloride channel 7 gene helps short-term hypersalinity stress resistance in Hong Kong oyster Crassostrea hongkongensis[J]. 海洋湖沼学报(英文), 2024,42(4):1261-1271. DOI：

PENG Yeshao,CHEN Ziao,DENG Qiong,et al.RNA interference reveals chloride channel 7 gene helps short-term hypersalinity stress resistance in Hong Kong oyster Crassostreahongkongensis[J].Journal of Oceanology and Limnology,2024,42(04):1261-1271. DOI：

摘要

Abstract

The chloride channel 7 gene (

CLC7

) of the Hong Kong oyster

Crassostrea

hongkongensis

was cloned and named

ChCLC7

. The cDNA was 2 572 bp in length

with a 5′ non-coding region containing 25 bp

a 3′ non-coding region containing 327 bp

and an open reading frame of 2 298 bp. ChCLC7 has 96.8% and 92.1% homology with CLC7 of

Crassostrea gigas

and

Crassostrea

virginica

respectively

and it was clustered with CLC7 of

gigas

and

virginica

. QRT-PCR showed that

ChCLC7

was expressed in all eight tissues

with the highest in adductor muscle and second in gill. The

ChCLC7

expression pattern in gill was altered significantly under high salinity stress with an overall upward and then downward trend. After RNA interference

the expression of

ChCLC7

and survival rate of oyster under high salinity stress was reduced significantly

and so did the concentration of hemolymph chloride ion in 48–96 h after RNA interference. We believed that

ChCLC7

could play an important role in osmoregulation of

hongkongensis

by regulating Cl‍⁻ transport. This study provided data for the analysis of molecular mechanism against oyster salinity stress.

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references

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RNA interference reveals chloride channel 7 gene helps short-term hypersalinity stress resistance in Hong Kong oyster Crassostrea hongkongensis

RNA interference reveals chloride channel 7 gene helps short-term hypersalinity stress resistance in Hong Kong oyster Crassostrea hongkongensis

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摘要

Abstract

关键词

Keywords

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