Effect of <italic style="font-style: italic">ChREBP</italic> on carbohydrate feeding regulation in mandarin fish <italic style="font-style: italic">Siniperca chuatsi</italic>

Jun YANG; Yulan KUANG; Xufang LIANG; Wenjing CAI; Jing XU; Junjie GAO; Junran WEI; Shan HE; Zengqiang YIN; Jinshui LIANG

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Effect of ChREBP on carbohydrate feeding regulation in mandarin fish Siniperca chuatsi

Aquaculture and Fisheries | Updated：2024-10-14

- Effect of ChREBP on carbohydrate feeding regulation in mandarin fish Siniperca chuatsi
- Effect of ChREBP on carbohydrate feeding regulation in mandarin fish Siniperca chuatsi
- 海洋湖沼学报（英文） 2023年41卷第5期页码：1988-1996
- Affiliations：
  
  1. College of Marine Technology and Environment, Dalian Ocean University, Dalian 116023, China
  2. College of Fisheries, Chinese Perch Research Center, Huazhong Agricultural University, Wuhan 430070, China
  3. Innovation Base for Chinese Perch Breeding, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan 430070, China
- Author bio：
  
  ** xfliang@mail.hzau.edu.cn
- Funds：
  
  the Earmarked Fund for the China Agriculture Research System(CARS-46);the National Key R&D Program of China(2018YFD0900400)
- DOI：
  中图分类号：
- 收稿：2021-11-22，
  
  修回：2022-02-08，
  
  录用：2022-05-27，
  
  纸质出版：2023
- Accepted：
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Effect of ChREBP on carbohydrate feeding regulation in mandarin fish Siniperca chuatsi[J]. 海洋湖沼学报（英文）, 2023,41(5):1988-1996.

Jun YANG, Yulan KUANG, Xufang LIANG, et al. Effect of ChREBP on carbohydrate feeding regulation in mandarin fish Siniperca chuatsi[J]. Journal of Oceanology and Limnology, 2023, 41(5): 1988-1996.
Effect of ChREBP on carbohydrate feeding regulation in mandarin fish Siniperca chuatsi[J]. 海洋湖沼学报（英文）, 2023,41(5):1988-1996. DOI：

Jun YANG, Yulan KUANG, Xufang LIANG, et al. Effect of ChREBP on carbohydrate feeding regulation in mandarin fish Siniperca chuatsi[J]. Journal of Oceanology and Limnology, 2023, 41(5): 1988-1996. DOI：

摘要

Abstract

Carnivorous fish have poor tolerance to carbohydrate in feed and low utilization rate of carbohydrate. Therefore

the balance of carbohydrate and lipids in the nutrient metabolism of carnivorous fish

the effective conversion and utilization of carbohydrate and lipids

and the feedback regulation of feeding are the key links for the efficient utilization of carnivorous fish feed. Carbohydrate response element binding protein (ChREBP) is a new transcription factor found in recent years in the glucose signaling pathway

and can also participate in feeding regulation. We performed in-vivo and in-vitro experiments to reveal the role of ChREBP in the glucose metabolism and feeding in mandarin fish. The mRNA expression of

ChREBP

and appetite regulatory factors were measured after intraperitoneal injection of glucose in mandarin fish

Siniperca chuatsi

and cotransfection with glucose and glucose+siRNA in the hypothalamic cells in mandarin fish. The results reveal that at hour 2 and 4 post intraperitoneal injection with 1 mg/g BW glucose

the blood glucose level of the mandarin fish increased significantly

but food intake decreased significantly

and it also displayed a significantly increased

ChREBP

mRNA expression levels in liver. At hour 4 post injection

hypothalamic

ChREBP

mRNA level was significantly increased

whereas the mRNA expressi

on levels of appetite genes neuropeptide Y (

npy

) and agouti-related peptide (

AgRP

) were decreased significantly. When the glucose concentration was 40 mmol/L

the expression level of

ChREBP

mRNA in mandarin fish hypothalamic cells was significantly up-regulated

but the expression level of appetite gene

npy

mRNA was down-regulated. When siRNA and glucose were co-transfected into mandarin fish brain cells

the expression level of chrebp mRNA was significantly decreased

and the appetite gene

npy

mRNA was significantly increased. The results indicated that glucose regulated food intake through the modulation of appetite gene

npy

by C

hREBP

关键词

Keywords

references

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Effect of ChREBP on carbohydrate feeding regulation in mandarin fish Siniperca chuatsi

Effect of ChREBP on carbohydrate feeding regulation in mandarin fish Siniperca chuatsi

DOI：

摘要

Abstract

关键词

Keywords

references