Correlation of gut microorganisms and non-volatile flavor substances provides new insights for breeding <italic style="font-style: italic">Scylla paramamosain</italic>

Gao GAO; Xing LI; Kangxiang QIN; Yun HU; Xiaosong JIANG; Chenxi CHE; Yuntao LI; Changkao MU; Chunlin WANG; Huan WANG

Your Location：

Home >

Browse articles >

Correlation of gut microorganisms and non-volatile flavor substances provides new insights for breeding Scylla paramamosain

Aquaculture and Fisheries | Updated：2024-10-11

- Correlation of gut microorganisms and non-volatile flavor substances provides new insights for breeding Scylla paramamosain
- Correlation of gut microorganisms and non-volatile flavor substances provides new insights for breeding Scylla paramamosain
- 海洋湖沼学报(英文) 2024年42卷第4期页码：1336-1347
- Affiliations：
  
  1.School of Marine Science, Ningbo University, Ningbo 315211, China
  2.Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo 315211, China
- Author bio：
  
  wanghuan1@nbu.edu.cn
- Funds：
  
  he National Natural Science Foundation of China(No. 42276106);the Youth Science and Technology Innovation Leading Talent Project of Ningbo City (No. 2023QL038), the Public Welfare Research Project of Ningbo(No. 2023S114);the “Three Rural Issues, Nine Parties” Agricultural Science and Technology Collaboration Plan of Zhejiang Province(No. 2024SNJF073);the Major Scientific and Technological Innovation Project of Wenzhou City(No. ZF2022008)
- DOI：
  中图分类号：
- 收稿：2023-07-27，
  
  网络首发：2023-10-07，
  
  纸质出版：2024-07-01
- Accepted：
Scan QR Code
Correlation of gut microorganisms and non-volatile flavor substances provides new insights for breeding Scylla paramamosain[J]. 海洋湖沼学报(英文), 2024,42(4):1336-1347.

GAO Gao,LI Xing,QIN Kangxiang,et al.Correlation of gut microorganisms and non-volatile flavor substances provides new insights for breeding Scylla paramamosain[J].Journal of Oceanology and Limnology,2024,42(04):1336-1347.
Correlation of gut microorganisms and non-volatile flavor substances provides new insights for breeding Scylla paramamosain[J]. 海洋湖沼学报(英文), 2024,42(4):1336-1347. DOI：

GAO Gao,LI Xing,QIN Kangxiang,et al.Correlation of gut microorganisms and non-volatile flavor substances provides new insights for breeding Scylla paramamosain[J].Journal of Oceanology and Limnology,2024,42(04):1336-1347. DOI：

摘要

Abstract

The farming of

Scylla

paramamosain

with specific flavors has a higher commercial value

and the flavors are related to the integrated farming environment and non-volatile flavor substances

while the survival environment is one of the important ways to source gut microorganisms in the organism. In this study

the levels of dominant taxa in the gut flora of

paramamosain

from Mong Cai

Vietnam (VN)

Taishan City

Guangdong Province (TS) of China

and Ninghai County

Ningbo City (NB) Zhejiang Province of China converged with those of

paramamosain

from Sanmen County

Ningbo City (CK1

CK2

and CK3) at 28 d of domestication. The top 15 genera with the highest abundance of VN

and NB gut flora were the same as CK1

CK2

and CK3

but with different percentages

and gr

adually converged to CK1

CK2

and CK3

respectively

at 28 d of domestication. Correlation between intestinal flora and non-volatile flavor substances in the hepatopancreas at the percentage level of relative abundance of bacterial genera found that above 28 d of domestication

Muribaculaceae

Psychrilyobacter

Clostridia_vadinBB60_group

Halarcobacter

Carboxylicivirga

Sediminispirochaeta

may be the most important genera affecting flavor amino acids of VN.

Sediminispirochaeta

Carboxylicivirga

Halarcobacter

Photobacterium

ZOR0006

Psychrilyobacter

and

Pseudomonas

may be the most important genera affecting flavor amino acids of NB.

Sediminispirochaeta

Carboxylicivirga

Halarcobacter

Photobacterium

ZOR0006

Vibrio

and

Sphingomonas

may be the most important genera affecting flavor amino acids of TS. These results show that the intestinal flora structure of crabs from different areas were domesticated in the same area for at least 28 d before they converged to that of the domesticated crab

and the most important genera affecting the flavor amino acids of TS

and NB were also identified. The results of this study provide a reference and basis for the technique of directional cultivation of the flavor quality of the crab.

关键词

Keywords

references

Bolger A M , Lohse M , Usadel B . 2014 . Trimmomatic: a flexible trimmer for Illumina sequence data . Bioinformatics , 30 ( 15 ): 2114 - 2120 , https://doi.org/10.1093/bioinformatics/btu170 https://doi.org/10.1093/bioinformatics/btu170 .

Caporaso J G , Kuczynski J , Stombaugh J et al . 2010 . QIIME allows analysis of high-throughput community sequencing data . Nature Methods , 7 ( 5 ): 335 - 336 , https://doi.org/10.1038/nmeth.f.303 https://doi.org/10.1038/nmeth.f.303 .

Cerdó T , Diéguez E , Campoy C . 2019 . Early nutrition and gut microbiome: interrelationship between bacterial metabolism, immune system, brain structure, and neurodevelopment . American Journal of Physiology-Endocrinology and Metabolism , 317 ( 4 ): E617 - E630 , https://doi.org/10.1152/ajpendo.00188.2019 https://doi.org/10.1152/ajpendo.00188.2019 .

Chen C Z , Li P , Liu L et al . 2022 . Exploring the interactions between the gut microbiome and the shifting surrounding aquatic environment in fisheries and aquaculture: a review . Environmental Research , 214 : 114202 , https://doi.org/10.1016/j.envres.2022.114202 https://doi.org/10.1016/j.envres.2022.114202 .

Chen X L , Yi H D , Liu S et al . 2021 . Probiotics improve eating disorders in ma ndarin fish ( Siniperca chuatsi ) induced by a pellet feed diet via stimulating immunity and regulating gut microbiota . Microorganisms , 9 ( 6 ): 1288 , https://doi.org/10.3390/microorganisms9061288 https://doi.org/10.3390/microorganisms9061288 .

Edgar R C . 2013 . UPARSE: highly accurate OTU sequences from microbial amplicon reads . Nature Methods , 10 ( 10 ): 996 - 998 , https://doi.org/10.1038/nmeth.2604 https://doi.org/10.1038/nmeth.2604 .

Gao J , Wang X W , Zou Z H et al . 2014 . Transcriptome analysis of the differences in gene expression between testis and ovary in green mud crab ( Scylla paramamosain ) . BMC Genomics , 15 ( 1 ): 585 , https://doi.org/10.1186/1471-2164-15-585 https://doi.org/10.1186/1471-2164-15-585 .

Huang X H , Hu J Y , Peng H N et al . 2022 . Embryonic exposure to tryptophan yields bullying victimization via reprogramming the microbiota-gut-brain axis in a chicken model . Nutrients , 14 ( 3 ): 661 , https://doi.org/10.3390/nu14030661 https://doi.org/10.3390/nu14030661 .

Ikhwanuddin M , Azmie G , Nahar S F et al . 2018 . Testis maturation stages of mud crab ( Scylla olivacea ) broodstock on different diets . Sains Malaysiana , 47 ( 3 ): 427 - 432 , https://doi.org/10.17576/jsm-2018-4703-01 https://doi.org/10.17576/jsm-2018-4703-01 .

Kang Y H , Yang B T , Hu R G et al . 2023 . Gut microbiota and metabolites may play a crucial role in sea cucumber Apostichopus japonicus aestivation . Microorganisms , 11 ( 2 ): 416 , https://doi.org/10.3390/microorganisms11020416 https://doi.org/10.3390/microorganisms11020416 .

Li C Q , Zhang B L , Wang X et al . 2019 . Improved utilization of soybean meal through fermentation with commensal Shewanella sp. MR-7 in turbot ( Scophthalmus maximus L.) . Microbial Cell Factories , 18 ( 1 ): 214 , https://doi.org/10.1186/s12934-019-1265-z https://doi.org/10.1186/s12934-019-1265-z .

Li E C , Xu C , Wang X D et al . 2018 . Gut microbiota and its modulation for healthy farming of Pacific white shrimp Litopenaeus vannamei . Reviews in Fisheries Science & Aquaculture , 26 ( 3 ): 381 - 399 , https://doi.org/10.1080/23308249.2018.1440530 https://doi.org/10.1080/23308249.2018.1440530 .

Lin H B , Yu X Y , Fang J X et al . 2018 . Flavor compounds in Pixian broad-bean paste: non-volatile organic acids and amino acids . Molecules , 23 ( 6 ): 1299 , https://doi.org/10.3390/molecules23061299 https://doi.org/10.3390/molecules23061299 .

Ling Z X , Cheng Y W , Yan X M et al . 2020 . Alterations of the fecal microbiota in Chinese patients with multiple sclerosis . Frontiers in Immunology , 11 : 590783 , https://doi.org/10.3389/fimmu.2020.590783 https://doi.org/10.3389/fimmu.2020.590783 .

Liu Q , Long Y N , Li B et al . 2020 . Rice-shrimp culture: a better intestinal microbiota, immune enzymatic activities, and muscle relish of crayfish ( Procambarus clarkii ) in Sichuan Province . Applied Microbiology and Biotechnology , 104 ( 21 ): 9413 - 9420 , https://doi.org/10.1007/s00253-020-10797-4 https://doi.org/10.1007/s00253-020-10797-4 .

Lozupone C , Knight R . 2005 . UniFrac: a new phylogenetic method for comparing microbial communities . Applied and Environmental Microbiology , 71 ( 12 ): 8228 - 8235 , https://doi.org/10.1128/AEM.71.12.8228-8235.2005 https://doi.org/10.1128/AEM.71.12.8228-8235.2005 .

Ma H Y , Jiang W , Liu P et al . 2014 . Identification of transcriptome-derived microsatellite markers and their association with the growth performance of the mud crab ( Scylla paramamosain ) . PLoS One , 9 ( 2 ): e89134 , https://doi.org/10.1371/journal.pone.0089134 https://doi.org/10.1371/journal.pone.0089134 .

Reyon D , Tsai S Q , Khayter C et al . 2012 . FLASH assembly of TALENs for high-throughput genome editing . Nature Biotechnology , 30 ( 5 ): 460 - 465 , https://doi.org/10.1038/nbt.2170 https://doi.org/10.1038/nbt.2170 .

Rudi K , Angell I L , Pope P B et al . 2018 . Stable core gut microbiota across the freshwater-to-saltwater transition for farmed Atlantic salmon . Applied and Environmental Microbiology , 84 ( 2 ): e01974 - 17 , https://doi.org/10.1128/AEM.01974-17 https://doi.org/10.1128/AEM.01974-17 .

Saqib H S A , Yuan Y , Kazmi S S U H et al . 2023 . Salinity gradients drove the gut and stomach microbial assemblages of mud crabs ( Scylla paramamosain ) in marine environments . Ecological Indicators , 151 : 110315 , https://doi.org/10.1016/j.ecolind.2023.110315 https://doi.org/10.1016/j.ecolind.2023.110315 .

Schloss P D , Westcott S L , Ryabin T et al . 2009 . Introducing Mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities . Applied and Environmental Microbiology , 75 ( 23 ): 7537 - 7541 , https://doi.org/10.1128/AEM.01541-09 https://doi.org/10.1128/AEM.01541-09 .

Shen G Q , Zhang X , Gong J et al . 2020 . Transcriptomic analysis of Procambarus clarkii affected by "Black May" disease . Scientific Reports , 10 ( 1 ): 21225 , https://doi.org/10.1038/s41598-020-78191-8 https://doi.org/10.1038/s41598-020-78191-8 .

Shi X , Lu J X , Wu Q Y et al . 2019 . Comparative a nalysis of growth performance between female and male mud crab Scylla paramamosain Crablets: evidences from a four-month successive growth experiment . Aquaculture , 505 : 351 - 362 , https://doi.org/10.1016/j.aquaculture.2019.02.062 https://doi.org/10.1016/j.aquaculture.2019.02.062 .

Sreelakshmi K R , Manjusha L , Vartak V R et al . 2016 . Variation in proximate composition and fatty acid profiles of mud crab meat with regard to sex and body parts . Indian Journal of Fisheries , 63 ( 2 ): 147 - 150 , https://doi.org/10.21077/ijf.2016.63.2.34511-23 https://doi.org/10.21077/ijf.2016.63.2.34511-23 .

Stephens R W , Arhire L , Covasa M . 2018 . Gut microbiota: from microorganisms to metabolic organ influencing obesity . Obesity , 26 ( 5 ): 801 - 809 , https://doi.‍org/10.1002/oby.22179 https://doi.‍org/10.1002/oby.22179 . https://do 10.1002/oby.22179 http://dx.doi.org/10.1002/oby.22179

Ticinesi A , Nouvenne A , Cerundolo N et al . 2019 . Gut microbiota, muscle mass and function in aging: a focus on physical frailty and sarcopenia . Nutrients , 11 ( 7 ): 1633 , https://doi.org/10.3390/nu11071633 https://doi.org/10.3390/nu11071633 .

Waiho K , Fazhan H , Quinitio E T et al . 2018 . Larval rearing of mud crab ( Scylla ): what lies ahead . Aquaculture , 493 : 37 - 50 , https://doi.org/10.1016/j.aquaculture.2018.04.047 https://doi.org/10.1016/j.aquaculture.2018.04.047 .

Wang G Z , Li S J , Zeng C S et al . 2005 . Status of biological studies and aquaculture development of the mud cra b, Scylla serrata , in China: an experimental ecological studies . Aquaculture International , 13 ( 5 ): 459 - 468 , https://doi.org/10.1007/s10499-005-1887-5 https://doi.org/10.1007/s10499-005-1887-5 .

Wang Q , Garrity G M , Tiedje J M et al . 2007 . Naïve Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy . Applied and Environmental Microbiology , 73 ( 16 ): 5261 - 5267 , https://doi.org/10.1128/AEM.00062-07 https://doi.org/10.1128/AEM.00062-07 .

Wang X Z , Tang B , Luo X et al . 2020 . Effects of temperature, diet and genotype-induced variations on the gut microbiota of abalone . Aquaculture , 524 : 735269 , https://doi.org/10.1016/j.aquaculture.2020.735269 https://doi.org/10.1016/j.aquaculture.2020.735269 .

Wang Y , Zhang C , Wang H et al . 2018 . Involvement of a newly identified atypical type II crustin ( Sp Crus5) in the antibacterial immunity of mud crab Scylla paramamosain . Fish & Shellfish Immunology , 75 : 346 - 356 , https://doi.‍org/10.1016/j.fsi.2018.02.026 https://doi.‍org/10.1016/j.fsi.2018.02.026 . https://do 10.1016/j.fsi.2018.02.026 http://dx.doi.org/10.1016/j.fsi.2018.02.026

Wu B L , Huang L , Chen J et al . 2021 . Gut microbiota of homologous Chinese soft-shell turtles ( Pelodiscus sinensis ) in different habitats . BMC Microbiology , 21 ( 1 ): 142 , https://doi.org/10.1186/s12866-021-02209-y https://doi.org/10.1186/s12866-021-02209-y .

Xiong J B . 2018 . Progress in the gut microbiota in exploring shrimp disease pathogenesis and incidence . Applied Microbiology and Biotechnology , 102 ( 17 ): 7343 - 7350 , https://doi.org/10.1007/s00253-018-9199-7 https://doi.org/10.1007/s00253-018-9199-7 .

Xu L , Zang E H , Sun S Y et al . 2022 . Main flavor compounds and molecular regulation mechanisms in fruits and vegetables . Critical Reviews in Food Science and Nutrition, published Online First , July 2022 , https://doi.org/10.1080/10408398.2022.2097195 https://doi.org/10.1080/10408398.2022.2097195 .

Ye H H , Tao Y , Wang G Z et al . 2011 . Experimental nursery culture of the mud crab Scylla paramamosain (Estampador) in China . Aquaculture International , 19 ( 2 ): 313 - 321 , https://doi.org/10.1007/s10499-010-9399-3 https://doi.org/10.1007/s10499-010-9399-3 .

Yu D X , Nguyen S M , Yang Y H et al . 2021 . Long-term diet quality is associated with gut microbiome diversity and composition among urban Chinese adults . The American Journal of Clinical Nutrition , 113 ( 3 ): 684 - 694 , https://doi.org/10.1093/ajcn/nqaa350 https://doi.org/10.1093/ajcn/nqaa350 .

Zhang M L , Sun Y H , Liu Y K et al . 2016 . Response of gut microbiota to salinity change in two euryhaline aquatic animals with reverse salinity preference . Aquaculture , 454 : 72 - 80 , https://doi.org/10.1016/j.aquaculture.2015.12.014 https://doi.org/10.1016/j.aquaculture.2015.12.014 .

Zhou K Q , Qin J Q , Pang H F et al . 2022 . Comparison of the composition and function of gut microbes between adult and juvenile Cipangopaludina chinensis in the rice sna il system . PeerJ , 10 : e13042 , https://doi.‍org/10.7717/peerj.13042 https://doi.‍org/10.7717/peerj.13042 . https://do 10.7717/peerj.13042 http://dx.doi.org/10.7717/peerj.13042

Zhu Z , Xu Y M , Liang J H et al . 2023 . Relationship of environmental factors in pond water and dynamic changes of gut microbes of sea bass Lateolabrax japonicus . Frontiers in Microbiology , 14 : 1086471 , https://doi.org/10.3389/fmicb.2023.1086471 https://doi.org/10.3389/fmicb.2023.1086471 .

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Transcriptomic insights into the adaptive mechanisms of antennal glands in mud crab (Scylla paramamosain) to acute chloride-type low-salinity alkaline water stress

Dopamine mediates the link between aggressive behavior and biogenic amine receptor genes in mud crab Scylla paramamosain

Dark environment affects circadian rhythms of gut bacterial community in the mud crab Scylla paramamosain

Alternative splicing genes/events play important role in adaptation of mud crab Scylla paramamosain to low salinity

基于LMI极点配置的高空台飞行环境模拟系统PI增益调度控制研究

Correlation of gut microorganisms and non-volatile flavor substances provides new insights for breeding Scylla paramamosain

Correlation of gut microorganisms and non-volatile flavor substances provides new insights for breeding Scylla paramamosain

DOI：

摘要

Abstract

关键词

Keywords

references