Combined proteomic and metabolomic studies on the liver of Amur sturgeon <italic style="font-style: italic">Acipenser</italic><italic style="font-style: italic">schrenckii</italic> under titanium dioxide nanoparticle exposure

Zhou ZHOU; Ting LIU; Jie KONG; Zhenxing ZHAO; Junquan ZHU

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Combined proteomic and metabolomic studies on the liver of Amur sturgeon Acipenserschrenckii under titanium dioxide nanoparticle exposure

Aquaculture and Fisheries | Updated：2024-10-14

- Combined proteomic and metabolomic studies on the liver of Amur sturgeon Acipenserschrenckii under titanium dioxide nanoparticle exposure
- Combined proteomic and metabolomic studies on the liver of Amur sturgeon Acipenserschrenckii under titanium dioxide nanoparticle exposure
- 海洋湖沼学报(英文) 2024年42卷第3期页码：1001-1015
- Affiliations：
  
  1. Key Laboratory of Aquacultural Biotechnology, Ministry of Education, and Key Laboratory of Marine Biotechnology of Zhejiang Province, College of Marine Sciences, Ningbo University, Ningbo 315000, China
  2. Guizhou Fisheries Research Institute, Guizhou Academy of Agriculture Sciences, Guiyang 550000, China
- Author bio：
  
  zhujunquan999@163.com
- Funds：
  
  the Guizhou Provincial Basic Research Program(ZK[2023]175);the Earmarked Fund for the China Agriculture Research System(CARS-46)
- DOI：
  中图分类号：
- 收稿：2023-07-20，
  
  录用：2023-08-28，
  
  网络首发：2023-09-18，
  
  纸质出版：2024-05-01
- Accepted：
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Combined proteomic and metabolomic studies on the liver of Amur sturgeon Acipenserschrenckii under titanium dioxide nanoparticle exposure[J]. 海洋湖沼学报(英文), 2024,42(3):1001-1015.

ZHOU Zhou,LIU Ting,KONG Jie,et al.Combined proteomic and metabolomic studies on the liver of Amur sturgeon Acipenser, schrenckii under titanium dioxide nanoparticle exposure[J].Journal of Oceanology and Limnology,2024,42(03):1001-1015.
Combined proteomic and metabolomic studies on the liver of Amur sturgeon Acipenserschrenckii under titanium dioxide nanoparticle exposure[J]. 海洋湖沼学报(英文), 2024,42(3):1001-1015. DOI：

ZHOU Zhou,LIU Ting,KONG Jie,et al.Combined proteomic and metabolomic studies on the liver of Amur sturgeon Acipenser, schrenckii under titanium dioxide nanoparticle exposure[J].Journal of Oceanology and Limnology,2024,42(03):1001-1015. DOI：

摘要

Abstract

Nanomaterials

particularly titanium dioxide nanoparticles (TiO

-NPs)

are extensively utilized across various industries. However

their environmental release has raised concerns regarding their potential ecological and environmental impacts. The reproductive toxicity of TiO

-NPs in fish species has attracted considerable attention

yet conflicting research outcomes have been reported. We investigated the effects of TiO

-NPs exposure on the liver of juvenile Amur sturgeon

Acipenser

schrenckii

using label-free proteomic and untargeted metabolomic analyses. The experiment included a control group and three groups exposed to different concentrations of TiO

-NPs (low

TL; medium

TM; high

TH). Compared to the control group

and 25 proteins and 35

and 158 metabolites were differentially expressed in the TH

and TL TiO

-NP-exposed groups

respectively. The differentially expressed genes (DEGs) were enriched in the Kyoto Encyclopedia for Genes and Genomes (KEGG) pathways related to glycolysis and gluconeogenesis. Moreover

among

the 126 correlated proteins

the most enriched pathways were associated with endocytosis and protein processing in the endoplasmic reticulum. Notably

syringic acid was significantly downregulated across all three TiO

-NP-exposed groups. To obtain a comprehensive overview of the TiO

-NP-induced expression changes

a co-regulated network of proteins and metabolites associated with TiO

-NPs exposure was constructed. Exposure to TiO

-NPs led to enrichment and alteration of pathways related to immune responses

including endocytosis

protein processing in the endoplasmic reticulum

and peroxisome proliferator-activated receptor (PPAR) signaling. In conclusion

our findings indicate that exposure to TiO

-NPs might disrupt glucose metabolism and induce immune responses

thus contributing to our understanding of the environmental impacts of nanomaterials and highlighting the need for further research and development of potential mitigation strategies.

关键词

Keywords

references

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Combined proteomic and metabolomic studies on the liver of Amur sturgeon Acipenserschrenckii under titanium dioxide nanoparticle exposure

Combined proteomic and metabolomic studies on the liver of Amur sturgeon Acipenserschrenckii under titanium dioxide nanoparticle exposure

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