Analysis of environmental selection pressure of superoxide dismutase in deep-sea sea cucumber

Yanan LI; Zongfu CHEN; Haibin ZHANG; Ruoyu LIU; Shuichun CHEN; Li LIN

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Analysis of environmental selection pressure of superoxide dismutase in deep-sea sea cucumber

Biology | Updated：2024-10-14

- Analysis of environmental selection pressure of superoxide dismutase in deep-sea sea cucumber
- Analysis of environmental selection pressure of superoxide dismutase in deep-sea sea cucumber
- 海洋湖沼学报(英文) 2024年42卷第3期页码：893-904
- Affiliations：
  
  1.Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510222, China
  2.Institute of Deep-sea Science and Engineering, Chinese Academy of Sciences, Sanya 572000, China
  3.College of Marine Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China
  4.Zhanjiang Tianli Sea Cucumber New Technology Development Co., Ltd., Zhanjiang 524000, China
- Author bio：
  
  linli@zhku.edu.cn
- Funds：
  
  the Guangdong Province Basic and Applied Basic Research Fund Project(2020A1515110826);the National Natural Science Foundation of China(42006115);the Major Scientific and Technological Projects of Hainan Province(ZDKJ2021036)
- DOI：
  中图分类号：
- 收稿：2023-06-19，
  
  录用：2023-07-14，
  
  网络首发：2023-09-06，
  
  纸质出版：2024-05-01
- Accepted：
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Analysis of environmental selection pressure of superoxide dismutase in deep-sea sea cucumber[J]. 海洋湖沼学报(英文), 2024,42(3):893-904.

LI Yanan,CHEN Zongfu,ZHANG Haibin,et al.Analysis of environmental selection pressure of superoxide dismutase in deep-sea sea cucumber[J].Journal of Oceanology and Limnology,2024,42(03):893-904.
Analysis of environmental selection pressure of superoxide dismutase in deep-sea sea cucumber[J]. 海洋湖沼学报(英文), 2024,42(3):893-904. DOI：

LI Yanan,CHEN Zongfu,ZHANG Haibin,et al.Analysis of environmental selection pressure of superoxide dismutase in deep-sea sea cucumber[J].Journal of Oceanology and Limnology,2024,42(03):893-904. DOI：

摘要

Abstract

Manganese superoxide dismutase (MnSOD) is an antioxidant that exists in mitochondria and can effectively remove superoxide anions in mitochondria. In a dark

high-pressure

and low-temperature deep-sea environment

MnSOD is essential for the survival of sea cucumbers. Six MnSODs were identified from the transcriptomes of deep and shallow-sea sea cucumbers. To explore their environmental adaptation mechanism

we conducted environmental selection pressure analysis through the branching site model of PAML software. We obtained night positive selection sites

and two of them were significant (97F→H

134K→V): 97F→H located in a highly conservative characteristic sequence

and its polarity change might have a great impact on the function of MnSOD; 134K→V had a change in piezophilic ability

which might help MnSOD adapt to the environment of high hydrostatic pressure in the deepsea. To further study the effect of these two positive selection sites on MnSOD

we predicted the point mutations of F97H and K134V on shallow-sea sea cucumber by using MAESTROweb and PyMOL. Results show that 97F→H

134K→V might improve MnSOD’s efficiency of scavenging superoxide anion and its ability to resist high hydrostatic pressure by moderately reducing its stability. The above results indicated that MnSODs of deep-sea sea cucumber adapted to deep-sea environments through their amino acid changes in polarity

piezophilic behavior

and local stability. This study revealed the correlation between MnSOD and extreme environment

and will help improve our understanding of the organism’s adaptation mechanisms in deep sea.

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