Seasonal changes in gut microbiota of sea cucumber over natural aestivation cycle

Ye ZHAO; Han WANG; Haona WANG; Lijie ZHANG; Hao SUN; Yanying ZHANG

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Seasonal changes in gut microbiota of sea cucumber over natural aestivation cycle

Ecology | Updated：2024-10-14

- Seasonal changes in gut microbiota of sea cucumber over natural aestivation cycle
- Seasonal changes in gut microbiota of sea cucumber over natural aestivation cycle
- 海洋湖沼学报(英文) 2024年42卷第3期页码：849-864
- Affiliations：
  
  Ocean School, Yantai University, Yantai 264005, China
- Author bio：
  
  zyzy19872006@163.com
  zhyanying@163.com
- Funds：
  
  the National Natural Science Foundation of China(41906098);the NSFC-Shandong Joint Fund(U2106208);the Development Plan of Youth Innovation Team in Colleges and Universities of Shandong Province(2022KJ269)
- DOI：
  中图分类号：
- 收稿：2023-04-26，
  
  录用：2023-06-05，
  
  网络首发：2023-08-08，
  
  纸质出版：2024-05-01
- Accepted：
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Seasonal changes in gut microbiota of sea cucumber over natural aestivation cycle[J]. 海洋湖沼学报(英文), 2024,42(3):849-864.

ZHAO Ye,WANG Han,WANG Haona,et al.Seasonal changes in gut microbiota of sea cucumber over natural aestivation cycle[J].Journal of Oceanology and Limnology,2024,42(03):849-864.
Seasonal changes in gut microbiota of sea cucumber over natural aestivation cycle[J]. 海洋湖沼学报(英文), 2024,42(3):849-864. DOI：

ZHAO Ye,WANG Han,WANG Haona,et al.Seasonal changes in gut microbiota of sea cucumber over natural aestivation cycle[J].Journal of Oceanology and Limnology,2024,42(03):849-864. DOI：

摘要

Abstract

Sea cucumber

Apostichopus

japonicus

is an ideal model organism for marine invertebrate aestivation; it annually enters a “s

leeping state” for nearly 3 months when ambient water temperature range is 25–30 °‍‍C. The natural fasting is accompanied by remodeling the intestinal biota and function

which is a part of host biology and could affect the gut microbiota. We investigatesd the impact of annual aestivation on gut microbiota using high-throughput sequencing of 16S rRNA amplicons. Results reveal a notable alteration in the composition of gut bacteria during aestivation during which various indigenous families and genera that exhibit a preference for dietary glycans (e.g.

family Rhodobacteraceae and Flavobacteriaceae) decreased

while the taxa capable of utilizing substrates derived from the host (e.g.

genus

Akkermansia

and

Prevotella

) increased

and so did certain opportunistic pathogenic bacteria. Moreover

the investigation delved into the gut morphology and immunity response of

japonicus

and reveal that the intestine of

japonicus

undergoes substantial atrophy and degeneration during aestivation. However

there was an observed augmentation in the levels of acid and neutral mucin within mucous cells

as well as an enhanced immune defense response (as evidenced by increased gene expression of

AjTLR3

LITAF

Ajp105

and

LYZ

). These results imply that the composition of sea cucumber gut microbiota differed between aestivation and active periods

which potentially affects the intestinal functions of the host and the symbiotic relationship between host and its microbiota over the active-aestivation cycle.

关键词

Keywords

references

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