<italic style="font-style: italic">Nannochloropsis</italic> artificial chromosomes (NannoACs) loom on the horizon

Li GUO; Guanpin YANG

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Nannochloropsis artificial chromosomes (NannoACs) loom on the horizon

Biology | Updated：2024-10-12

- Nannochloropsis artificial chromosomes (NannoACs) loom on the horizon
- Nannochloropsis artificial chromosomes (NannoACs) loom on the horizon
- 海洋湖沼学报(英文) 2023年41卷第6期页码：2336-2347
- Affiliations：
  
  1.College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
  2.Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China
  3.Key Laboratory of Marine Genetics and Breeding of Ministry of Education, Ocean University of China, Qingdao 266003, China
- Author bio：
  
  yguanpin@ouc.edu.cn
- Funds：
  
  the National Key R&D Program of China(2018YFD0901506;2018YFD0900305);the Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)(2018 SDKJ0406-3)
- DOI：
  中图分类号：
- 收稿：2022-08-30，
  
  录用：2022-10-13，
  
  网络首发：2022-10-24，
  
  纸质出版：2023-11-01
- Accepted：
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Nannochloropsis artificial chromosomes (NannoACs) loom on the horizon[J]. 海洋湖沼学报(英文), 2023,41(6):2336-2347.

GUO Li,YANG Guanpin.Nannochloropsis artificial chromosomes (NannoACs) loom on the horizon[J].Journal of Oceanology and Limnology,2023,41(06):2336-2347.
Nannochloropsis artificial chromosomes (NannoACs) loom on the horizon[J]. 海洋湖沼学报(英文), 2023,41(6):2336-2347. DOI：

GUO Li,YANG Guanpin.Nannochloropsis artificial chromosomes (NannoACs) loom on the horizon[J].Journal of Oceanology and Limnology,2023,41(06):2336-2347. DOI：

摘要

Abstract

Species in genus

Nannochloropsis

especially

oceanica

and

gaditana

have been evolving as the model microalgae for both application and theory studies. The position effect of genome

integration

the carrying capability limitation of integrative vectors and the instability of non-integrative vectors have hindered

Nannochloropsis

genetic modification with concatenate genes and extremely long DNA fragments. The molecular tools including genetic transformation

homologous recombination

gene edition

gene stacking and episome vectors for transient gene expression and diverse reporters and selection markers have been rapidly developing in

Nannochloropsis

species. The construction of animal and plant artificial chromosomes with “top down” strategy has set fine examples for the construction of

Nannochloropsis

artificial chromosomes (NannoACs). It seems that the methods and materials to set the foundation for constructing NannoACs are at hands. In this review

we outlined the current status of transgenes in

Nannochloropsis

species

summarized the limitations of both integrative and non-integrative vectors

and proposed a tentative approach to construct NannoACs by doubling and stabilizing the genome first

and then truncating the natural chromosomes. NannoACs once constructed will facilitate transferring the desired traits and concatenate genes into

Nannochloropsis

genetic backgrounds

thus contributing towards its genetic improvement and synthetic biological studies.

关键词

Keywords

references

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Nannochloropsis artificial chromosomes (NannoACs) loom on the horizon

Nannochloropsis artificial chromosomes (NannoACs) loom on the horizon

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