Environmental hypoxia induces apoptosis in large yellow croaker <italic style="font-style: italic">Larimichthys</italic><italic style="font-style: italic">crocea</italic> via both intrinsic and extrinsic pathways

Shengyu LUO; Cheng LIU; Xinming GAO; Jingqian WANG; Yibo ZHANG; Jie DING; Congcong HOU; Junquan ZHU; Bao LOU; Weiliang SHEN; Xiongfei WU; Chundan ZHANG

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Environmental hypoxia induces apoptosis in large yellow croaker Larimichthyscrocea via both intrinsic and extrinsic pathways

Aquaculture and Fisheries | Updated：2024-10-12

- Environmental hypoxia induces apoptosis in large yellow croaker Larimichthyscrocea via both intrinsic and extrinsic pathways
- Environmental hypoxia induces apoptosis in large yellow croaker Larimichthyscrocea via both intrinsic and extrinsic pathways
- 海洋湖沼学报(英文) 2023年41卷第6期页码：2429-2443
- Affiliations：
  
  1. Key Laboratory of Applied Marine Biotechnology by the Ministry of Education, School of Marine Sciences, Ningbo University, Ningbo 315211, China
  2. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
  3. State Key Laboratory of Large Yellow Croaker Breeding, Ningbo Academy of Oceanology and Fishery, Ningbo 315012, China
- Author bio：
  
  zhujunquan@nbu.edu.cn
  loubao6577@163.com
- Funds：
  
  the NSFC-Zhejiang Joint Fund for the Integration of Industrialization and Informatization(U1809212);the Scientific and Technical Project of Zhejiang Province(2021C02069-1-2);the Science and Technology Innovation 2025 Major Special Project of Ningbo City(2021Z002);the Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, and the K. C. Wong Magna Fund in Ningbo University
- DOI：
  中图分类号：
- 收稿：2022-07-04，
  
  录用：2022-08-19，
  
  网络首发：2022-10-19，
  
  纸质出版：2023-11-01
- Accepted：
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Environmental hypoxia induces apoptosis in large yellow croaker Larimichthyscrocea via both intrinsic and extrinsic pathways[J]. 海洋湖沼学报(英文), 2023,41(6):2429-2443.

LUO Shengyu,LIU Cheng,GAO Xinming,et al.Environmental hypoxia induces apoptosis in large yellow croaker Larimichthys, crocea via both intrinsic and extrinsic pathways[J].Journal of Oceanology and Limnology,2023,41(06):2429-2443.
Environmental hypoxia induces apoptosis in large yellow croaker Larimichthyscrocea via both intrinsic and extrinsic pathways[J]. 海洋湖沼学报(英文), 2023,41(6):2429-2443. DOI：

LUO Shengyu,LIU Cheng,GAO Xinming,et al.Environmental hypoxia induces apoptosis in large yellow croaker Larimichthys, crocea via both intrinsic and extrinsic pathways[J].Journal of Oceanology and Limnology,2023,41(06):2429-2443. DOI：

摘要

Abstract

Hypoxia has become an unfavorable factor affecting the sustainable development of the large yellow croaker

Larimichthys

crocea

an economically important mariculture fish in China. Apoptosis is a consequence of hypoxia on fish. However

the effects of hypoxia stress on apoptosis in

crocea

remain largely unknown. We investigated the effect of environmental hypoxia on apoptosis in

crocea

. Results show that hypoxia induced apoptosis in

crocea

both in vivo and in vitro. The mitochondrial membrane potential was significantly reduced in large yellow croaker fry (LYCF) cells. The expression levels of B-cell lymphoma/leukemia-2 (Bcl-2) mRNA and protein were also significantly decreased in the liver and LYCF cells during 96 h and 48 h of hypoxia stress

respectively

whereas the expression level of B

cl-2 associated X (

Bax

) mRNA

Casp3

mRNA

and activity of caspase-3/7/9 were significantly increased

indicating that hypoxia induced caspase-dependent intrinsic apoptosis in

crocea

. The expression level of the apoptosis-inducing factor (AIF) protein was significantly increased in the liver and LYCF cells. The level of AIF protein was significantly decreased in the cytoplasm but increased in the nuclei of

crocea

demonstrating that hypoxia induced the AIF-mediated caspase-independent intrinsic apoptosis. In addition

the activity of caspase-8 was significantly increased

indicating that hypoxia stress induced extrinsic apoptosis in

crocea

. Therefore

hypoxia induced apoptosis in

crocea

through both the intrinsic and extrinsic pathways. The present study accumulated basic biological information to help elucidate the mechanism of hypoxia response in marine fish.

关键词

Keywords

references

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Environmental hypoxia induces apoptosis in large yellow croaker Larimichthyscrocea via both intrinsic and extrinsic pathways

Environmental hypoxia induces apoptosis in large yellow croaker Larimichthyscrocea via both intrinsic and extrinsic pathways

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摘要

Abstract

关键词

Keywords

references