Distribution of chromophytic phytoplankton in the Western Subarctic Gyre of Pacific Ocean revealed by morphological observation and <italic style="font-style: italic">rbc</italic>L gene sequences

Tao JIANG; Xiaohan QIN; Guannan WU; Huaxian ZHAO; Xiaotao YU; Xueyan XIAO; Wenjing LIU; Qingjing HU; Jufa CHEN; Nan LI

Your Location：

Home >

Browse articles >

Distribution of chromophytic phytoplankton in the Western Subarctic Gyre of Pacific Ocean revealed by morphological observation and rbcL gene sequences

Ecology | Updated：2024-10-12

- Distribution of chromophytic phytoplankton in the Western Subarctic Gyre of Pacific Ocean revealed by morphological observation and rbcL gene sequences
- Distribution of chromophytic phytoplankton in the Western Subarctic Gyre of Pacific Ocean revealed by morphological observation and rbcL gene sequences
- 海洋湖沼学报(英文) 2023年41卷第6期页码：2166-2179
- Affiliations：
  
  1.School of Ocean, Yantai University, Yantai 264005, China
  2.Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China
  3.Key Laboratory of Ministry of Education for Environment Change and Resources Use in Beibu Gulf, Guangxi Key Laboratory of Earth Surface Processes and Intelligent Simulation, Nanning Normal University, Nanning 530001, China
  4.State Key Laboratory of Acoustics, Institute of Acoustics, Chinese Academy of Sciences, Beijing 100190, China
  5.School of Medicine, Yunnan University, Kunming 650091, China
  6.College of Ocean and Meteorology, Guangdong Ocean University, Zhanjiang 524088, China
- Author bio：
  
  chenjf@ysfri.ac.cn
  nli0417@163.com
- Funds：
  
  the National Key Research and Development Program of China(2019YFD0901401);the National Natural Science Foundation of China(42176206;81900630);the Natural Science Foundation of Shandong Province(ZR2021MD071);the “One Hundred Talents” Project of Guangxi(6020303891251);the Outstanding Youth Project of Yunnan Provincial Department of Science and Technology(2019F1019)
- DOI：
  中图分类号：
- 收稿：2022-04-19，
  
  录用：2022-08-22，
  
  网络首发：2022-10-15，
  
  纸质出版：2023-11-01
- Accepted：
Scan QR Code
Distribution of chromophytic phytoplankton in the Western Subarctic Gyre of Pacific Ocean revealed by morphological observation and rbcL gene sequences[J]. 海洋湖沼学报(英文), 2023,41(6):2166-2179.

JIANG Tao,QIN Xiaohan,WU Guannan,et al.Distribution of chromophytic phytoplankton in the Western Subarctic Gyre of Pacific Ocean revealed by morphological observation and rbcL gene sequences[J].Journal of Oceanology and Limnology,2023,41(06):2166-2179.
Distribution of chromophytic phytoplankton in the Western Subarctic Gyre of Pacific Ocean revealed by morphological observation and rbcL gene sequences[J]. 海洋湖沼学报(英文), 2023,41(6):2166-2179. DOI：

JIANG Tao,QIN Xiaohan,WU Guannan,et al.Distribution of chromophytic phytoplankton in the Western Subarctic Gyre of Pacific Ocean revealed by morphological observation and rbcL gene sequences[J].Journal of Oceanology and Limnology,2023,41(06):2166-2179. DOI：

摘要

Abstract

Western Subarctic Gyre (WSG)

which possesses distinctive differences in oceanographic and biogeochemical processes

is situated in the northwest subarctic Pacific. The WSG is characterized by high nutrient and low chlorophyll. We carried out a field investigation in this area in summer 2020 and performed microscopic observation

cytometric counting

and RuBisCO large subunit (

rbc

L) gene analysis to understand the community structure and spatial distribution of chromophytic phytoplankton better. Microscopic method revealed that total phytoplankton (

10 μm

including Bacillariophyta

Dinoflagellata

Ochrophyta

and Chlorophyta) abundances ranged (0.6×10

)–(167.4×10

) cells/L with an increasing trend from south to north. Dinoflagellates and Pennatae diatoms dominated the phytoplankton assemblages in the southern and northern stations

respectively. Major chromophytic phytoplankton groups derived from

rbc

L genes included Haptophyta

Ochrophyta

Bacillariophyta

as well as rarely occurring groups

such as Xanthophyta

Cyanobacteria

Dinoflagellata

Rhodophyta

and Cryptophyta. At the phylum level

Haptophyta was the most abundant phylum

accounting for approximately 30.80% of the total obtained operational taxonomic units in all samples. Ochrophyta and Bacillariophyta were the second and third most abundant phylum

and their relative abundance was 20.26% and 19.60%

respectively. Further

redundancy analysis showed that high proportion of diatoms (e.g.

microscopic and

rbc

L methods) was posit

ively correlated with nutrients (e.g.

dissolved inorganic nitrogen (DIN)

dissolved inorganic phosphorous

and dissolved silicate (DSi)) and negatively correlated with temperature and salinity. The proportion of Ochrophyta

Rhodophyta

and Cyanobateria identified by

rbc

L genes was positively correlated with salinity and temperature and showed negative correlation to nutrients. This work is the first molecular study of phytoplankton accomplished in the WSG

and our results show some discrepancies between morphological observation and

rbc

L gene sequences

which highlight the necessity of combining the microscopic and molecular methods to reveal the diversity of phytoplankton in marine environment.

关键词

Keywords

references

Aizawa C , Tanimoto M , Jordan R W . 2005 . Living diatom assemblages from North Pacific and Bering Sea surface waters during summer 1999 . Deep Sea Research Part II : Topical Studies in Oceanography , 52 ( 16-18 ): 2186 - 2205 , https://doi.org/10.1016/j.dsr2.2005.08.008 https://doi.org/10.1016/j.dsr2.2005.08.008 .

Bolyen E , Rideout J R , Dillon M R et al . 2019 . Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2 . Nature Biotechnology , 37 ( 8 ): 852 - 857 , https://doi.org/10.1038/s41587-019-0209-9 https://doi.org/10.1038/s41587-019-0209-9 .

Bradley I M , Pinto A J , Guest J S . 2016 . Design and evaluation of Illumina MiSeq-compatible, 18S rRNA gene-specific primers for improved characterization of mixed phototrophic communities . Applied and Environmental Microbiology , 82 ( 19 ): 5878 - 5891 , https://doi.org/10.1128/AEM.01630-16 https://doi.org/10.1128/AEM.01630-16 .

Buck K R , Chavez F P , Campbell L . 1996 . Basin-wide distributions of living carbon components and the inverted trophic pyramid of the central gyre of the North Atlantic Ocean, summer 1993 . Aquatic Microbial Ecology , 10 ( 3 ): 283 - 298 . https://do 10.3354/ame010283 http://dx.doi.org/10.3354/ame010283

Eikrem W , Romari K , Latasa M et al . 2004 . Florenciella parvula gen. et sp. nov. (Dictyochophyceae, Heterokontophyta), a small flagellate isolated from the English Channel . Phycologia , 43 ( 6 ): 658 - 668 , https://doi.org/10.2216/i0031-8884-43-6-658.1 https://doi.org/10.2216/i0031-8884-43-6-658.1 .

Eiler A , Drakare S , Bertilsson S et al . 2013 . Unveiling distribution patterns of freshwater phytoplankton by a next generation sequencing based approach . PLoS One , 8 ( 1 ): e53516 , https://doi.org/10.1371/journal.pone.0053516 https://doi.org/10.1371/journal.pone.0053516 .

Fujiki T , Hosoda S , Harada N . 2022 . Phytoplankton blooms in summer and autumn in the northwestern subarctic Pacific detected by the mooring and float systems . Journal of Oceanography , 78 ( 2 ): 63 - 72 , https://doi.org/10.1007/s10872-021-00628-z https://doi.org/10.1007/s10872-021-00628-z .

Fujiki T , Matsumoto K , Honda M C et al . 2009 . Phytoplankton composition in the subarctic North Pacific during autumn 2005 . Journal of Plankton Research , 31 ( 2 ): 179 - 191 , https://doi.org/10.1093/plankt/fbn108 https://doi.org/10.1093/plankt/fbn108 .

Fujiki T , Matsumoto K , Mino Y et al . 2014 . Seasonal cycle of phytoplankton community structure and photophysiological state in the western subarctic gyre of the North Pacific . Limnology and Oceanography , 59 ( 3 ): 887 - 900 , https://doi.org/10.4319/lo.2014.59.3.0887 https://doi.org/10.4319/lo.2014.59.3.0887 .

Giesbrecht K E , Varela D E , Wiktor J et al . 2019 . A decade of summertime measurements of phytoplankton biomass, productivity and assemblage composition in the Pacific Arctic Region from 2006 to 2016 . Deep Sea Research Part II : Topical Studies in Oceanography , 162 : 93 - 113 . https://do 10.1016/j.dsr2.2018.06.010 http://dx.doi.org/10.1016/j.dsr2.2018.06.010

Harrison P J , Whitney F A , Tsuda A et al . 2004 . Nutrient and plankton dynamics in the NE and NW Gyres of the Subarctic Pacific Ocean . Journal of Oceanography , 60 ( 1 ): 93 - 117 . https://do 10.1023/b:joce.0000038321.57391.2a http://dx.doi.org/10.1023/b:joce.0000038321.57391.2a

Hattori H , Koike M , Tachikawa K et al . 2004 . Spatial variability of living coccolithophore distribution in the western subarctic Pacific and western Bering Sea . Journal of Oceanography , 60 ( 2 ): 505 - 515 , https://doi.org/10.1023/B:JOCE.0000038063.81738.ab https://doi.org/10.1023/B:JOCE.0000038063.81738.ab .

Ichinomiya M , Dos Santos A L , Gourvil P et al . 2016 . Diversity and oceanic distribution of the Parmales (Bolidophyceae), a picoplanktonic group closely related to diatoms . The ISME Journal , 10 ( 10 ): 2419 - 2434 , https://doi.org/10.1038/ismej.2016.38 https://doi.org/10.1038/ismej.2016.38 .

Ichinomiya M , Yoshikawa S , Kamiya M et al . 2011 . Isolation and characterization of Parmales (Heterokonta/Heterokontophyta/Stramenopiles) from the Oyashio region, Western North Pacific . Journal of Phycology , 47 ( 1 ): 144 - 151 , https://doi.org/10.1111/j.1529-8817.2010.00926.x https://doi.org/10.1111/j.1529-8817.2010.00926.x .

Imai K , Nojiri Y , Tsurushima N et al . 2002 . Time series of seasonal variation of primary productivity at station KNOT (44°‍N, 155°‍E) in the sub-arctic western North Pacific . Deep Sea Research Part II : Topical Studies in Oceanography , 49 ( 24-25 ): 5395 - 5408 . https://do 10.1016/s0967-0645(02)00198-4 http://dx.doi.org/10.1016/s0967-0645(02)00198-4

Jiang T , Chai C , Wang J F et al . 2016 . Temporal and spatial variations of abundance of phycocyanin- and phycoerythrin-rich Synechococcus in Pearl River Estuary and adjacent coastal area . Journal of Ocean University of China , 15 ( 5 ): 897 - 904 , https://doi.org/10.1007/s11802-016-3011-z https://doi.org/10.1007/s11802-016-3011-z .

John D E , Patterson S S , Paul J H . 2007 . Phytoplankton-group specific quantitative polymerase chain reaction assays for RuBisCO mRNA transcripts in seawater . Marine Biotechnology , 9 ( 6 ): 747 - 759 , https://doi.org/10.1007/s10126-007-9027-z https://doi.org/10.1007/s10126-007-9027-z .

Karlson B , Andersen P , Arneborg L et al . 2021 . Harmful algal blooms and their effects in coastal seas of northern Europe . Harmful Algae , 102 : 101989 , https://doi.org/10.1016/j.hal.2021.101989 https://doi.org/10.1016/j.hal.2021.101989 .

Komuro C , Narita H , Imai K et al . 2005 . Microplankton assemblages at Station KNOT in the subarctic western Pacific , 1999 - 2000 . Deep Sea Research Part II : Topical Studies in Oceanography , 52 ( 16-18 ): 2206 - 2217 , https://doi.org/10.1016/j.dsr2.2005.08.006 https://doi.org/10.1016/j.dsr2.2005.08.006 .

Kwak J H , Lee S H , Hwang J et al . 2014 . Summer primary productivity and phytoplankton community composition driven by different hydrographic structures in the East/Japan Sea and the Western Subarctic Pacific . Journal of Geophysical Research : Oceans , 119 ( 7 ): 4505 - 4519 , https://doi.org/10.1002/2014jc009874 https://doi.org/10.1002/2014jc009874 .

Li B L , Karl D M , Letelier R M et al . 2013 . Variability of chromophytic phytoplankton in the North Pacific Subtropical Gyre . Deep Sea Research Part II : Topical Studies in Oceanography , 93 : 84 - 95 , https://doi.org/10.1016/j.dsr2.2013.03.007 https://doi.org/10.1016/j.dsr2.2013.03.007 .

Li N , Yu S X , Wang Y C et al . 2016 . Diversity of phototrophic phytoplankton in Northern South China Sea indicated by rbcL analysis . Journal of Applied Phycology , 28 ( 2 ): 773 - 781 , https://doi.org/10.1007/s10811-015-0624-3 https://doi.org/10.1007/s10811-015-0624-3 .

Liu H , Probert I , Uitz J et al . 2009 . Extreme diversity in noncalcifying haptophytes explains a major pigment paradox in open oceans . Proceedings of the National Academy of Sciences of the United States of America , 106 ( 31 ): 12803 - 12808 , https://doi.org/10.1073/pnas.0905841106 https://doi.org/10.1073/pnas.0905841106 .

Liu H B , Imai K , Suzuki K et al . 2002a . Seasonal variability of picophytoplankton and bacteria in the western subarctic Pacific Ocean at station KNOT . Deep Sea Research Part II : Topical Studies in Oceanography , 49 ( 24-25 ): 5409 - 5420 , https://doi.org/10.1016/S0967-0645(02)00199-6 https://doi.org/10.1016/S0967-0645(02)00199-6 .

Liu H B , Suzuki K , Minami C et al . 2002b . Picoplankton community structure in the subarctic Pacific Ocean and the Bering Sea during summer 1999 . Marine Ecology Progress Series , 237 : 1 - 14 , https://doi.org/10.3354/meps 237001 https://doi.org/10.3354/meps237001 .

Liu H B , Suzuki K , Saito H . 2004 . Community structure and dynamics of phytoplankton in the western subarctic Pacific Ocean: a synthesis . Journal of Oceanography , 60 ( 1 ): 119 - 137 , https://doi.org/10.1023/B:JOCE.0000038322.79644.36 https://doi.org/10.1023/B:JOCE.0000038322.79644.36 .

Liu Y J , Yang G P , Guan X J et al . 2006 . Genetic diversity and its seasonal variation of jiaozhou bay phytoplankton determined by rbcL gene sequencing . Acta Oceanologica Sinica , 25 ( 2 ): 125 - 134 .

Mackenzie L A , Smith K F , Rhodes L L et al . 2011 . Mortalities of sea-cage salmon ( Oncorhynchus tshawytscha ) due to a bloom of Pseudochattonella verruculosa (Dictyochophyceae) in Queen Charlotte Sound, New Zealand . Harmful Algae , 11 : 45 - 53 , https://doi.org/10.1016/j.hal.2011.07.003 https://doi.org/10.1016/j.hal.2011.07.003 .

Mardones J I , Fuenzalida G , Zenteno K et al . 2019 . Salinity-growth response and ichthyotoxic potency of the Chilean Pseudochattonella verruculosa . Frontiers in Marine Science , 6 : 24 , https://doi.org/10.3389/fmars.2019.00024 https://doi.org/10.3389/fmars.2019.00024 .

Matsumoto K , Honda M C , Sasaoka K et al . 2014 . Seasonal variability of primary production and phytoplankton biomass in the western pacific subarctic gyre: control by light availability within the mixed layer . Journal of Geophysical Research : Oceans , 119 ( 9 ): 6523 - 6534 , https://doi.org/10.1002/2014JC009982 https://doi.org/10.1002/2014JC009982 .

Mochizuki M , Shiga N , Saito M et al . 2002 . Seasonal changes in nutrients, chlorophyll a and the phytoplankton assemblage of the western subarctic gyre in the Pacific Ocean . Deep Sea Research Part II : Topical Studies in Oceanography , 49 ( 24-25 ): 5421 - 5439 , https://doi.org/10.1016/S0967-0645(02)00209-6 https://doi.org/10.1016/S0967-0645(02)00209-6 .

Morse D , Salois P , Markovic P et al . 1995 . A nuclear-encoded form II RuBisCO in dinoflagellates . Science , 268 ( 5217 ): 1622 - 1624 , https://doi.org/10.1126/science.7777861 https://doi.org/10.1126/science.7777861 .

Nishioka J , Hirawake T , Nomura D et al . 2021 . Iron and nutrient dynamics along the East Kamchatka Current, western Bering Sea Basin and Gulf of Anadyr . Progress in Oceanography , 198 : 102662 , https://doi.org/10.1016/j.pocean.2021.102662 https://doi.org/10.1016/j.pocean.2021.102662 .

Nishioka J , Obata H , Ogawa H et al . 2020 . Sub-polar marginal seas fuel the North Pacific through the intermediate water at the termination of the global ocean circulation . Proceedings of the National Academy of Sciences of the United States of America , 117 ( 23 ): 12665 - 12673 , https://doi.org/10.1073/pnas.2000658117 https://doi.org/10.1073/pnas.2000658117 .

Nishioka J , Ono T , Saito H et al . 2011 . Oceanic iron supply mechanisms which support the spring diatom bloom in the Oyashio region, western subarctic Pacific . Journal of Geophysical Research : Oceans , 116 ( C2 ): C02021 . https://do 10.1029/2010jc006321 http://dx.doi.org/10.1029/2010jc006321

Nishioka J , Takeda S , Kudo I et al . 2003 . Size-fractionated iron distributions and iron-limitation processes in the subarctic NW Pacific . Geophysical Research Letters , 30 ( 14 ): 1730 , https://doi.org/10.1029/2002GL016853 https://doi.org/10.1029/2002GL016853 .

Obayashi Y , Tanoue E , Suzuki K et al . 2001 . Spatial and temporal variabilities of phytoplankton community structure in the northern North Pacific as determined by phytoplankton pigments . Deep Sea Research Part I : Oceanographic Research Papers , 48 ( 2 ): 439 - 469 , https://doi.org/10.1016/S0967-0637(00)00036-4 https://doi.org/10.1016/S0967-0637(00)00036-4 .

Park M G , Kim S , Kim H S et al . 2006 . First successful culture of the marine dinoflagellate Dinophysis acuminata . Aquatic Microbial Ecology , 45 ( 2 ): 101 - 106 , https://doi.org/10.3354/ame045101 https://doi.org/10.3354/ame045101 .

Pujari L , Narale D , Kan J J et al . 2021 . Distribution of chromophytic phytoplankton in the eddy-induced upwelling region of the west Pacific Ocean revealed using rbc L genes . Frontiers in Microbiology , 12 : 596015 , https://doi.org/10.3389/fmicb.2021.596015 https://doi.org/10.3389/fmicb.2021.596015 .

Pujari L , Wu C , Kan J J et al . 2019 . Diversity and spatial distribution of chromophytic phytoplankton in the Bay of Bengal revealed by RuBisCO genes ( rbc L) . Frontiers in Microbiology , 10 : 1501 , https://doi.org/10.3389/fmicb.2019.01501 https://doi.org/10.3389/fmicb.2019.01501 .

Rii Y M , Bidigare R R , Church M J . 2018 . Differential responses of eukaryotic phytoplankton to nitrogenous nutrients in the North Pacific Subtropical Gyre . Frontiers in Marine Science , 5 : 92 , https://doi.org/10.3389/fmars.2018.00092 https://doi.org/10.3389/fmars.2018.00092 .

Roden G I . 1991 . Subarctic-subtropical transition zone of the North Pacific: large-scale aspects and mesoscale structure . NOAA Technical Report NMFS , 105 ( 1 ): 1 - 38 , https://doi.org/10.5194/bg-5-353-2008 https://doi.org/10.5194/bg-5-353-2008 .

Rognes T , Flouri T , Nichols B et al . 2016 . VSEARCH: a versatile open source tool for metagenomics . PeerJ , 4 : e2584 , https://doi.org/10.7717/peerj.2584 https://doi.org/10.7717/peerj.2584 .

Samanta B , Bhadury P . 2014 . Analysis of diversity of chromophytic phytoplankton in a mangrove ecosystem using rbc L gene sequencing . Journal of Phycology , 50 ( 2 ): 328 - 340 , https://doi.org/10.1111/jpy.12163 https://doi.org/10.1111/jpy.12163 .

Samanta B , Bhadury P . 2016 . A comprehensive framework for functional diversity patterns of marine chromophytic phytoplankton using rbc L phylogeny . Scientific Reports , 6 ( 1 ): 20783 , https://doi.org/10.1038/srep20783 https://doi.org/10.1038/srep20783 .

Sarthou G , Timmermans K R , Blain S et al . 2005 . Growth physiology and fate of diatoms in the ocean: a review . Journal of Sea Research , 53 ( 1-2 ): 25 - 42 , https://doi.org/10.1016/j.seares.2004.01.007 https://doi.org/10.1016/j.seares.2004.01.007 .

Strickland J D H , Parsons T R . 1972 . A Practical Handbook of Seawater Analysis . Fisheries Research Board of Canada, Ottawa . 310 p.

Suzuki K , Minami C , Liu H B et al . 2002 . Temporal and spatial patterns of chemotaxonomic algal pigments in the subarctic Pacific and the Bering Sea during the early summer of 1999 . Deep Sea Research Part II : Topical Studies in Oceanography , 49 ( 24-25 ): 5685 - 5704 , https://doi.org/10.1016/S0967-0645(02)00218-7 https://doi.org/10.1016/S0967-0645(02)00218-7 .

Taylor F J R , Waters R E . 1982 . Spring phytoplankton in the subarctic North Pacific Ocean . Marine Biology , 67 ( 3 ): 323 - 335 , https://doi.org/10.1007/BF00397673 https://doi.org/10.1007/BF00397673 .

Tsurushima N , Nojiri Y , Imai K et al . 2002 . Seasonal variations of carbon dioxide system and nutrients in the surface mixed layer at station KNOT (44°‍N, 155°‍E) in the subarctic western North Pacific . Deep Sea Research Part II : Topical Studies in Oceanography , 49 ( 24-25 ): 5377 - 5394 , https://doi.org/10.1016/S0967-0645(02)00197-2 https://doi.org/10.1016/S0967-0645(02)00197-2 .

Uitz J , Claustre H , Gentili B et al . 2010 . Phytoplankton class-specific primary production in the world's oceans: seasonal and Interannual variability from satellite observations . Global Biogeochemical Cycles , 24 ( 3 ): GB 3016 , https://doi.org/10.1029/2009gb003680 https://doi.org/10.1029/2009gb003680 .

Utermöhl H . 1958 . Methods of collecting plankton for various purposes are discussed . SIL Communications , 1953 - 1996 , 9 ( 1 ): 1 - 38 , https://doi.org/10.1080/05384680.1958.11904091 https://doi.org/10.1080/05384680.1958.11904091

Waga H , Fujiwara A , Hirawake T et al . 2022 . Primary productivity and phytoplankton community structure in surface waters of the western subarctic Pacific and the Bering Sea during summer with reference to bloom stages . Progress in Oceanography , 201 : 102738 , https://doi.org/10.1016/j.pocean.2021.102738 https://doi.org/10.1016/j.pocean.2021.102738 .

Xu H H , Tabita F R . 1996 . Ribulose-1,5-bisphosphate carboxylase/oxygenase gene expression and diversity of Lake Erie planktonic microorganisms . Applied and Environmental Microbiology , 62 ( 6 ): 1913 - 1921 , https://doi.org/10.1128/aem.62.6.1913-1921.1996 https://doi.org/10.1128/aem.62.6.1913-1921.1996 .

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

The summer pattern of phytoplankton pigment assemblages in response to water masses in the Yellow Sea

Long-term dynamics of net-phytoplankton community under environmental drivers in Jiaozhou Bay, China

Standing crop and size structure of phytoplankton are strongly regulated by the turbidity maximum in the macrotidal Qiantang River estuary-Hangzhou Bay

Selection of protocols for phytoplankton pigment analysis: a comparative study

V形前缘对激波入射边界层流动影响的数值模拟与分析

Distribution of chromophytic phytoplankton in the Western Subarctic Gyre of Pacific Ocean revealed by morphological observation and rbcL gene sequences

Distribution of chromophytic phytoplankton in the Western Subarctic Gyre of Pacific Ocean revealed by morphological observation and rbcL gene sequences

DOI：

摘要

Abstract

关键词

Keywords

references