Short communication When nets meet environmental DNA metabarcoding: integrative approach to unveil invertebrate community patterns of hypersaline lakes

Matthew A. CAMPBELL; Alex LAINI; Nicole E. WHITE; Morten E. ALLENTOFT; Mattia SACCÒ

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Short communication When nets meet environmental DNA metabarcoding: integrative approach to unveil invertebrate community patterns of hypersaline lakes

Special Section on Salt Lake Research | Updated：2024-10-14

- Short communication When nets meet environmental DNA metabarcoding: integrative approach to unveil invertebrate community patterns of hypersaline lakes
- Short communication When nets meet environmental DNA metabarcoding: integrative approach to unveil invertebrate community patterns of hypersaline lakes
- 海洋湖沼学报(英文) 2023年41卷第4期页码：1331-1340
- Affiliations：
  
  1.Trace and Environmental DNA (TrEnD) Laboratory, School of Molecular and Life Sciences, Curtin University, Perth 6102, WA, Australia
  2.Department of Life Sciences and Systems Biology, University of Turin, Turin 10124, Italy
  3.Subterranean Research and Groundwater Ecology (SuRGE) Group, Trace and Environmental DNA (TrEnD) Laboratory, School of Molecular and Life Sciences, Curtin University, Perth 6102, WA, Australia
  4.Lundbeck Foundation GeoGenetics Centre, Globe Institute, University of Copenhagen, Copenhagen 1353, Denmark
- Author bio：
  
  mattia.sacco@curtin.edu.au
- Funds：
  
  the Curtin-BHP alliance within the framework of the “eDNA for Global Environment Studies (eDGES)” program
- DOI：
  中图分类号：
- 收稿：2022-03-30，
  
  录用：2022-08-13，
  
  纸质出版：2023-07-01
- Accepted：
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Short communication When nets meet environmental DNA metabarcoding: integrative approach to unveil invertebrate community patterns of hypersaline lakes[J]. 海洋湖沼学报(英文), 2023,41(4):1331-1340.

CAMPBELL Matthew A.,LAINI Alex,WHITE Nicole E.,et al.Short communication When nets meet environmental DNA metabarcoding: integrative approach to unveil invertebrate community patterns of hypersaline lakes[J].Journal of Oceanology and Limnology,2023,41(04):1331-1340.
Short communication When nets meet environmental DNA metabarcoding: integrative approach to unveil invertebrate community patterns of hypersaline lakes[J]. 海洋湖沼学报(英文), 2023,41(4):1331-1340. DOI：

CAMPBELL Matthew A.,LAINI Alex,WHITE Nicole E.,et al.Short communication When nets meet environmental DNA metabarcoding: integrative approach to unveil invertebrate community patterns of hypersaline lakes[J].Journal of Oceanology and Limnology,2023,41(04):1331-1340. DOI：

摘要

Abstract

Saline and hypersaline wetlands account for almost half of the volume of inland water globally. They provide pivotal habitat for a vast range of species

including crucial ecosystem services for humans such as carbon sink storage and extractive resource reservoirs. Despite their importance

effective ecological assessment is in its infancy compared to current conventional surveys carried out in freshwater ecosystems. The integration of environmental DNA (eDNA‍) analysis and traditional techniques has the potential to transform biomonitoring processes

particularly in remote and understudied saline environments. In this context

this preliminary study aims to explore the potential of eDNA coupled with conventional approaches by targeting five hypersaline lakes at Rottnest Island (Wadjemup) in Western Australia. We focused on the invertebrate community

a widely accepted key ecological indicator to assess the conservational status in rivers and lakes. The combination of metabarcoding with morphology-based taxonomic analysis described 16 taxa belonging to the orders Anostraca

Diptera

Isopoda

and Coleoptera. DNA-based diversity assessment revealed more taxa at higher taxonomic resolution than the morphology-based taxonomic analysis. However

certain taxa (i.‍e.

Ephydridae

Stratyiomidae

Ceratopogonidae) were only identified via net surveying. Overall

our results indicate that great potential resides in combining conventional net-based surveys with novel eDNA approaches in saline and hypersaline lakes. Indeed

urgent and effective conservational frameworks are required to contrast the enormous pressure that these ecosystems are increasingly facing. Further investigations at larger spatial-temporal scales will allow consolidation of robust

reliable

and affordable biomonitoring frameworks in the underexplored world of saline wetlands.

关键词

Keywords

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