Molecular identification and biochemical characteristics of a delta class glutathione S-transferase gene (FcδGST) from Chinese shrimp <italic style="font-style: italic">Fenneropenaeus</italic><italic style="font-style: italic">chinensis</italic>

Jiaobing LI; Yan WANG; Jingjie HU; Yajin XU; Qingqian ZHOU; Lu ZHANG; Mengqiang WANG

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Molecular identification and biochemical characteristics of a delta class glutathione S-transferase gene (FcδGST) from Chinese shrimp Fenneropenaeuschinensis

Biology | Updated：2024-10-14

- Molecular identification and biochemical characteristics of a delta class glutathione S-transferase gene (FcδGST) from Chinese shrimp Fenneropenaeuschinensis
- Molecular identification and biochemical characteristics of a delta class glutathione S-transferase gene (FcδGST) from Chinese shrimp Fenneropenaeuschinensis
- 海洋湖沼学报(英文) 2023年41卷第5期页码：1940-1953
- Affiliations：
  
  1. MOE Key Laboratory of Marine Genetics and Breeding (Qingdao 266003), and Key Laboratory of Tropical Aquatic Germplasm of Hainan Province of Sanya Oceanographic Institution (Sanya 572024), Ocean University of China, Qingdao 266071, China
  2. Laboratory for Marine Fisheries Science and Food Production Processes, and Center for Marine Molecular Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China
  3. Hainan Yazhou Bay Seed Laboratory, Sanya 572024, China
- Author bio：
  
  wangmengqiang@ouc.edu.cn
- Funds：
  
  the Project of Sanya Yazhou Bay Science and Technology City Management Foundation(SKJC-KJ-2019KY01);the Startup Fund of Young Talents Project of Ocean University of China
- DOI：
  中图分类号：
- 收稿：2022-07-08，
  
  录用：2022-08-07，
  
  网络首发：2022-08-17，
  
  纸质出版：2023-09-01
- Accepted：
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Molecular identification and biochemical characteristics of a delta class glutathione S-transferase gene (FcδGST) from Chinese shrimp Fenneropenaeuschinensis[J]. 海洋湖沼学报(英文), 2023,41(5):1940-1953.

LI Jiaobing,WANG Yan,HU Jingjie,et al.Molecular identification and biochemical characteristics of a delta class glutathione S-transferase gene (FcδGST) from Chinese shrimp Fenneropenaeus, chinensis[J].Journal of Oceanology and Limnology,2023,41(05):1940-1953.
Molecular identification and biochemical characteristics of a delta class glutathione S-transferase gene (FcδGST) from Chinese shrimp Fenneropenaeuschinensis[J]. 海洋湖沼学报(英文), 2023,41(5):1940-1953. DOI：

LI Jiaobing,WANG Yan,HU Jingjie,et al.Molecular identification and biochemical characteristics of a delta class glutathione S-transferase gene (FcδGST) from Chinese shrimp Fenneropenaeus, chinensis[J].Journal of Oceanology and Limnology,2023,41(05):1940-1953. DOI：

摘要

Abstract

Glutathione S-transferases (GSTs) are a superfamily of multifun

ction enzymes involved in the regulation of redox homeostasis and innate immune responses against various pathogenic infections in marine invertebrates. In the present study

a delta class GST gene (designated as FcδGST) was cloned from

Fenneropenaeus

chinensis

using rapid amplification of cDNA ends (RACE) technology. The complete cDNA sequence of FcδGST was 780 bp in length

which includes a 27-bp 5′ non-coding region (UTR)

a 117-bp 3′ UTR

a 636-bp open reading frame (ORF)

and a polyadenylate signal site (AATAAA) presented at the upstream of poly A tail. The FcδGST gene encoded 211 amino acids peptide

including a GST_N domain and a GST_C domain

and exhibited high similarity with previously reported delta GSTs. The predicted molecular mass of FcδGST protein was 23.39 kDa

and its theoretical isoelectric point (pI) was 5.34. The FcδGST mRNA transcripts were ubiquitously expressed in all the tested tissues

with the highest expression level in hemocytes and hepatopancreas. During the stimulation of

Vibrio

anguillarum

or white spot syndrome virus (WSSV)

the mRNA expression of FcδGST in hemocytes and hepatopancreas revealed significant up-regulation. The purified recombinant FcδGST protein (designated as rFcδGST) exhibited specific catalytic activity against 1-chloro-2

4-dinitrobenzene (CDNB) substrate with relatively low stable enzymatic activities. These results indicated that FcδGST was a fragile but typical novel delta class GST member and potentially involved in the innate immune responses of

chinensis

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references

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Molecular identification and biochemical characteristics of a delta class glutathione S-transferase gene (FcδGST) from Chinese shrimp Fenneropenaeuschinensis

Molecular identification and biochemical characteristics of a delta class glutathione S-transferase gene (FcδGST) from Chinese shrimp Fenneropenaeuschinensis

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