Transcriptomes of <italic style="font-style: italic">Litopenaeus</italic> <italic style="font-style: italic">vannamei</italic> reveal modulation of antioxidant system induced by dietary archaeal carotenoids

Wei XIE; Guoru DU; Honggang DENG; Yingchao MA; Meirong GAO; Hu DUAN; Sung YIK YEONG; Liying SUI

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Transcriptomes of Litopenaeus vannamei reveal modulation of antioxidant system induced by dietary archaeal carotenoids

Aquaculture and Fisheries | Updated：2024-10-14

- Transcriptomes of Litopenaeus vannamei reveal modulation of antioxidant system induced by dietary archaeal carotenoids
- Transcriptomes of Litopenaeus vannamei reveal modulation of antioxidant system induced by dietary archaeal carotenoids
- 海洋湖沼学报(英文) 2023年41卷第5期页码：2010-2019
- Affiliations：
  
  1.State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, China
  2.Asian Regional Artemia Reference Center, College of Marine and Environmental Sciences, Tianjin University of Science and Technology, Tianjin 300457, China
  3.Institute of Marine Biotechnology (IMB), Universiti Malaysia Terengganu (UMT), Kuala Nerus, Terengganu 21030, Malaysia
- Author bio：
  
  suily@tust.edu.cn
- Funds：
  
  the Project of China One-Belt-One-Road Foreign Expert Research Collaboration, Ministry of Science and Technology, China(DL2021002001L);the Open Project Program of State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology(SKLFNS-KF-202205)
- DOI：
  中图分类号：
- 收稿：2022-06-05，
  
  录用：2022-07-11，
  
  网络首发：2022-08-17，
  
  纸质出版：2023-09-01
- Accepted：
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Transcriptomes of Litopenaeus vannamei reveal modulation of antioxidant system induced by dietary archaeal carotenoids[J]. 海洋湖沼学报(英文), 2023,41(5):2010-2019.

XIE Wei,DU Guoru,DENG Honggang,et al.Transcriptomes of ,Litopenaeus,vannamei reveal modulation of antioxidant system induced by dietary archaeal carotenoids[J].Journal of Oceanology and Limnology,2023,41(05):2010-2019.
Transcriptomes of Litopenaeus vannamei reveal modulation of antioxidant system induced by dietary archaeal carotenoids[J]. 海洋湖沼学报(英文), 2023,41(5):2010-2019. DOI：

XIE Wei,DU Guoru,DENG Honggang,et al.Transcriptomes of ,Litopenaeus,vannamei reveal modulation of antioxidant system induced by dietary archaeal carotenoids[J].Journal of Oceanology and Limnology,2023,41(05):2010-2019. DOI：

摘要

Abstract

Oxidative stress induced by factors such as ammonia nitrogen has become a major issue in shrimp farming. The effects of carotenoids on the growth and antioxidant capability of

Litopenaeus

vannamei

juveniles were investigated in this study using dietary archaeal carotenoids supplementation. For four weeks

shrimp were given diets containing 0 mg/kg (Ctrl) and 55.98 mg/kg (Car) archaeal carotenoids. Dietary archaeal carotenoids significantly enhanced the astaxanthin content in shrimp muscles and carapaces

as well as the superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity (

0.05). The malonaldehyde (MDA) content in Car group significantly decreased (

0.05). The transcriptome analysis was conducted to determine the molecular processes in response to archaeal carotenoids supplementation. A total of 1 536 differentially expressed genes (DEG

s) were detected

including 538 upregulated DEGs and 998 downregulated DEGs. GO functional enrichment analysis between Ctrl and Car indicated that 26 GO terms including extracellular region

metabolic process

and proteolysis were enriched. The KEGG pathway enrichment analysis revealed that the amino sugar and nucleotide sugar metabolism

cysteine and methionine metabolism

glycine serine and threonine metabolism

and amino acid biosynthesis were enriched. Archaeal carotenoids influenced the expression of several important genes involved in reactive oxygen species (ROS) generation

Nrf2 signaling

and antioxidant enzymes. Seven DEGs were chosen to confirm the RNA-Seq data using qRT-PCR. The genes and pathways discovered in this work assist to elucidate the molecular processes through which archaeal carotenoid enhances

vannamei

antioxidative system.

关键词

Keywords

references

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Transcriptomes of Litopenaeus vannamei reveal modulation of antioxidant system induced by dietary archaeal carotenoids

Transcriptomes of Litopenaeus vannamei reveal modulation of antioxidant system induced by dietary archaeal carotenoids

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摘要

Abstract

关键词

Keywords

references