Characterization of the sphingolipid profiling of <italic style="font-style: italic">Emiliania huxleyi</italic> against virus infection

Jingwen LIU; Jingjing GAO; Enquan ZHANG; Hanrui JIANG; Guiling LI; Jian LI; Jun ZENG; Daren WU

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Characterization of the sphingolipid profiling of Emiliania huxleyi against virus infection

Biology | Updated：2024-10-14

- Characterization of the sphingolipid profiling of Emiliania huxleyi against virus infection
- Characterization of the sphingolipid profiling of Emiliania huxleyi against virus infection
- 海洋湖沼学报(英文) 2023年41卷第4期页码：1547-1557
- Affiliations：
  
  College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China
- Author bio：
  
  zjxm69602@yeah.net
  darrenwu20@163.com
- Funds：
  
  the National Natural Science Foundation of China(42076086;41576166);the Natural Science Foundation of Fujian Province(2020J05138);the Education and Research Project for Young and Middle-aged Teachers of Fujian Province(JAT190343);the Cultivation Plan for Distinguished Young Scholars in Fujian Universities
- DOI：
  中图分类号：
- 收稿：2022-01-07，
  
  录用：2022-05-05，
  
  网络首发：2022-05-27，
  
  纸质出版：2023-07-01
- Accepted：
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Characterization of the sphingolipid profiling of Emiliania huxleyi against virus infection[J]. 海洋湖沼学报(英文), 2023,41(4):1547-1557.

LIU Jingwen,GAO Jingjing,ZHANG Enquan,et al.Characterization of the sphingolipid profiling of ,Emiliania huxleyi against virus infection[J].Journal of Oceanology and Limnology,2023,41(04):1547-1557.
Characterization of the sphingolipid profiling of Emiliania huxleyi against virus infection[J]. 海洋湖沼学报(英文), 2023,41(4):1547-1557. DOI：

LIU Jingwen,GAO Jingjing,ZHANG Enquan,et al.Characterization of the sphingolipid profiling of ,Emiliania huxleyi against virus infection[J].Journal of Oceanology and Limnology,2023,41(04):1547-1557. DOI：

摘要

Abstract

Lipidomics approach by UPLC-Q-Exactive-MS was used for the identification

quantification

comparison

and characterization of sphingolipids in virus infected marine

Emiliania huxleyi

BOF92 cells. The results show that 16 significantly changed sphingolipids (including Cer

CerG1

and SPHm) were identified during viral infection. Our data confirmed previously recognized facts that viral infection led to a shift toward virus-specific sphingolipids

which is consistent with the down-regulation of genes involved in the host de novo sphingolipid biosynthesis. Moreover

we revealed the upregulation of virus-encoded homologous genes participating in de novo sphingolipids biosynthesis and virus-specific hydroxylated long chain bases (LCBs) as phytoCer

suggesting the competitive inhibition of host sphingolipid synthesis to produce the required building blocks for viral production

replication

and assembly. Additionally

Cer 40꞉1;2

Cer 40꞉2;2 isomer

and CerG1 39꞉0;2

Cer 39꞉0;2 as novel metabolite markers might indicate the general dysfunctions in

huxleyi

in response to viral infection. Our results show that viral infection led to a profound remodeling of host sphingolipidome

by which viruses depend on the hijacking of host sphingolipid metabolism to support the viral life cycle.

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Keywords

references

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Characterization of the sphingolipid profiling of Emiliania huxleyi against virus infection

Characterization of the sphingolipid profiling of Emiliania huxleyi against virus infection

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摘要

Abstract

关键词

Keywords

references