Characterization of SARS-COV-2 main protease inhibitory peptides from <italic style="font-style: italic">Ulva</italic> <italic style="font-style: italic">prolifera</italic> proteins

Zhiyong LI; Yehua WANG; Caiwei FU; Dongren ZHANG; Tuanjie CHE; Songdong SHEN

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Characterization of SARS-COV-2 main protease inhibitory peptides from Ulva prolifera proteins

Biology | Updated：2024-10-14

- Characterization of SARS-COV-2 main protease inhibitory peptides from Ulva prolifera proteins
- Characterization of SARS-COV-2 main protease inhibitory peptides from Ulva prolifera proteins
- 海洋湖沼学报(英文) 2023年41卷第5期页码：1887-1899
- Affiliations：
  
  1.School of Biology & Basic Medical Sciences, Soochow University, Suzhou 215101, China
  2.Key Laboratory of Functional Genomic and Molecular Diagnosis of Gansu Province, Lanzhou 730030, China
- Author bio：
  
  shensongdong@suda.edu.cn
- Funds：
  
  the National Key R&D Program of China(2016YFC1402102);the National Natural Science Foundation of China(41976109);the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- DOI：
  中图分类号：
- 收稿：2022-04-27，
  
  录用：2022-06-09，
  
  网络首发：2022-07-05，
  
  纸质出版：2023-09-01
- Accepted：
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Characterization of SARS-COV-2 main protease inhibitory peptides from Ulva prolifera proteins[J]. 海洋湖沼学报(英文), 2023,41(5):1887-1899.

LI Zhiyong,WANG Yehua,FU Caiwei,et al.Characterization of SARS-COV-2 main protease inhibitory peptides from Ulvaprolifera proteins[J].Journal of Oceanology and Limnology,2023,41(05):1887-1899.
Characterization of SARS-COV-2 main protease inhibitory peptides from Ulva prolifera proteins[J]. 海洋湖沼学报(英文), 2023,41(5):1887-1899. DOI：

LI Zhiyong,WANG Yehua,FU Caiwei,et al.Characterization of SARS-COV-2 main protease inhibitory peptides from Ulvaprolifera proteins[J].Journal of Oceanology and Limnology,2023,41(05):1887-1899. DOI：

摘要

Abstract

The main protease (M

pro

) is essential for the replication of SARS-COV-2 and therefore represents a promising anti-viral target. In this study

we screened M

pro

inhibitory peptides from

Ulva

prolifera

protein on in-silico proteolysis. Cytotoxicity analysis using the online toxic prediction tool ToxinPred revealed that all the peptides were non-cytotoxic. The hexapeptide (SSGFID) exhibited high M

pro

inhibitory activity in molecular docking and its IC

value was 139.40±0.82 μmol/L in vitro according to fluorescence resonance energy transfer assay (FRET). Quantitative real-time (qRT-) PCR results show that SSGFID could stimulate the expression of mitosis-related factors

including nuclear factor-κB

cyclin D1

and cyclin-dependent kinase 4

to promote the proliferation of mice splenocytes. Stability study revealed that SSGFID showed resistance against pepsin and trypsin but lost D (Asp) after pretreatment at 121 °‍‍C for 15 min. Besides

SSGFID was mainly transported through the Caco-2 cell monolayer by the peptide transporter PepT1 and passive-mediated transport during the transport study. Unfortunately

the peptide was also degraded by Caco-2 intracellular enzymes

and the transfer rate of intact peptide was 4.2%. Furthermore

Lineweaver–Burk plots demonstrated that SSGFID possessed a mixed inhibitory characteristic with M

pro

. Our study indicated the potential of

Ulva

prolifera

as antiviral and immune-enhancing functional food ingredients and nutraceuticals.

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Keywords

references

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Characterization of SARS-COV-2 main protease inhibitory peptides from Ulva prolifera proteins

Characterization of SARS-COV-2 main protease inhibitory peptides from Ulva prolifera proteins

DOI：

摘要

Abstract

关键词

Keywords

references