A pilot survey on antimicrobial activity and diversity of soil-derived actinobacteria from different depths in Gudzhirganskoe saline lake in Eastern Siberia

Qin YANG; Puyu GUO; Elena Y. ABIDUEVA; Elena V. LAVRENTYEVA; Irina M. LISEVICH; Ilya A. OSTERMAN; Petr V. SERGIEV; Shaowei LIU; Chunmei XUE; Chenghang SUN

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A pilot survey on antimicrobial activity and diversity of soil-derived actinobacteria from different depths in Gudzhirganskoe saline lake in Eastern Siberia

Special Section on Salt Lake Research | Updated：2024-10-14

- A pilot survey on antimicrobial activity and diversity of soil-derived actinobacteria from different depths in Gudzhirganskoe saline lake in Eastern Siberia
- A pilot survey on antimicrobial activity and diversity of soil-derived actinobacteria from different depths in Gudzhirganskoe saline lake in Eastern Siberia
- 海洋湖沼学报(英文) 2023年41卷第4期页码：1307-1320
- Affiliations：
  
  1.Department of Microbial Chemistry, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China
  2.Beijing Key Laboratory of Antimicrobial Agents, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China
  3.College of Life Sciences, Jiamusi University, Jiamusi 154000, China
  4.Institute of General and Experimental Biology, Siberian Branch, Russian Academy of Sciences, Ulan-Ude 670047, Russia
  5.Center of Life Sciences, Skolkovo Institute of Science and Technology, Moscow 101135, Russia
  6.Department of Chemistry, A. N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 101135, Russia
- Author bio：
  
  jmsxcm@126.com
  chenghangsun@hotmail.com
- Funds：
  
  the CAMS Innovation Fund for Medical Sciences(CIFMS 2021-I2M-1-028);the Beijing Natural Science Foundation(7222256);the National Natural Science Foundation of China(82011530051);the Kunlun Talented People, High-end Innovation and Entrepreneurship talents Plan, Qinghai Province, China, for Chenghang SUN, the Talent Training Project of Basic Research Fund of Heilongjiang Department of Education Item to Chunmei XUE(2020-KYYWF-0238);the Sino-Hungarian Bilateral S&T Cooperation Projects from 2019 to 2020(Chinese Project: 8-11);partly supported by the Russian Foundation for Basic Research (Nos. NCFS-RFBR 20-54-53014, 18-44-030021 r_а), the Russian Science Foundation(20-74-10031);partially within the framework of the State Assignment(121030100229-1);for Institute of General and Experimental Biology, Siberian Branch, Russian Academy of Sciences
- DOI：
  中图分类号：
- 收稿：2022-03-15，
  
  录用：2022-06-06，
  
  网络首发：2022-06-30，
  
  纸质出版：2023-07-01
- Accepted：
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A pilot survey on antimicrobial activity and diversity of soil-derived actinobacteria from different depths in Gudzhirganskoe saline lake in Eastern Siberia[J]. 海洋湖沼学报(英文), 2023,41(4):1307-1320.

YANG Qin,GUO Puyu,ABIDUEVA Elena Y.,et al.A pilot survey on antimicrobial activity and diversity of soil-derived actinobacteria from different depths in Gudzhirganskoe saline lake in Eastern Siberia[J].Journal of Oceanology and Limnology,2023,41(04):1307-1320.
A pilot survey on antimicrobial activity and diversity of soil-derived actinobacteria from different depths in Gudzhirganskoe saline lake in Eastern Siberia[J]. 海洋湖沼学报(英文), 2023,41(4):1307-1320. DOI：

YANG Qin,GUO Puyu,ABIDUEVA Elena Y.,et al.A pilot survey on antimicrobial activity and diversity of soil-derived actinobacteria from different depths in Gudzhirganskoe saline lake in Eastern Siberia[J].Journal of Oceanology and Limnology,2023,41(04):1307-1320. DOI：

摘要

Abstract

To explore pharmaceutical actinobacteria from salty environments in Siberia

8 soil samples were collected from different depths of Gudzhirganskoe saline lake

the Republic of Buryatia

Russia. Totally

635 actinobacterial strains affiliated with 21 genera in 12 families of 7 orders were obtained by culture-dependent approaches. The predominant genus was

Streptomyces

(74.5%)

followed by

Microbacterium

(4.9%)

Agromyces

(3.5%)

Nocardiopsis

(3.0%)

and

Kitasatospora

(2.5%). Seven strains showed relatively low 16S rRNA similarities (

98.65%) with validly described species. Seventy-seven strains were selected as representatives to evaluate their antimicrobial activities and underlying mechanisms by paper-disk diffusion method and a double fluorescent protein reporter system (pDualrep2)

respectively.

Streptomyces

sp. S6b3-1 was highlighted due to its strong inhibitory activities against Gram-positive bacteria and underlying antiba

cterial mechanism by inducing the SOS response in the reporter system. LG-1

(

)-6-[1-(benzoyloxy) ethyl

]

-phenazine-1-carboxylic acid

an analogue of saphenamycin was finally isolated from the culture broth of

Streptomyces

strain. This study preliminarily explored the actinobacterial diversity of soil samples collected in the highly alkaline and saline lake Gudzhirganskoe by culture-dependent approach and their capacity to produce antibiotics for the first time

which revealed that it deserves to make more efforts to discover new actinobacterial species and potential new antibiotics from the saline lake in Eastern Siberia.

关键词

Keywords

references

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