Expression and localization of vitellogenin genes (VTG) and receptor (VGR) in the gonad development of silver pomfret <italic style="font-style: italic">Pampus</italic> <italic style="font-style: italic">argenteus</italic>

Zitao XIONG; Jiazhe YANG; Shun ZHANG; Yajun WANG; Shanliang XU; Chunyang GUO; Danli WANG

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Expression and localization of vitellogenin genes (VTG) and receptor (VGR) in the gonad development of silver pomfret Pampus argenteus

Biology | Updated：2024-10-14

- Expression and localization of vitellogenin genes (VTG) and receptor (VGR) in the gonad development of silver pomfret Pampus argenteus
- Expression and localization of vitellogenin genes (VTG) and receptor (VGR) in the gonad development of silver pomfret Pampus argenteus
- 海洋湖沼学报(英文) 2023年41卷第4期页码：1575-1592
- Affiliations：
  
  1.School of Marine Sciences, Ningbo University, Ningbo 315211, China
  2.Key Laboratory of Applied Marine Biotechnology, Ningbo University, Chinese Ministry of Education, Ningbo 315211, China
- Author bio：
  
  guochunyang@nbu.edu.cn
  wangdanli@nbu.edu.cn
- Funds：
  
  the National Natural Science Foundation of China(31872586;42076118);the Major Project of Science, Technology and Innovation 2025 in Ningbo City(2021Z003);the K. C. Wong Magna Fund in Ningbo University
- DOI：
  中图分类号：
- 收稿：2022-01-12，
  
  纸质出版：2023-07-01
- Accepted：
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Expression and localization of vitellogenin genes (VTG) and receptor (VGR) in the gonad development of silver pomfret Pampus argenteus[J]. 海洋湖沼学报(英文), 2023,41(4):1575-1592.

XIONG Zitao,YANG Jiazhe,ZHANG Shun,et al.Expression and localization of vitellogenin genes (VTG) and receptor (VGR) in the gonad development of silver pomfret Pampusargenteus[J].Journal of Oceanology and Limnology,2023,41(04):1575-1592.
Expression and localization of vitellogenin genes (VTG) and receptor (VGR) in the gonad development of silver pomfret Pampus argenteus[J]. 海洋湖沼学报(英文), 2023,41(4):1575-1592. DOI：

XIONG Zitao,YANG Jiazhe,ZHANG Shun,et al.Expression and localization of vitellogenin genes (VTG) and receptor (VGR) in the gonad development of silver pomfret Pampusargenteus[J].Journal of Oceanology and Limnology,2023,41(04):1575-1592. DOI：

摘要

Abstract

Vitellogenesis is the main event of oocyte growth in oviparous animals

which is mainly manifested by the accumulation of vitellogenin (VTG). The accumulation of vitellogenin depends mainly on the absorption of exogenous vitellogenin

which enters oocyte through endocytosis mediated by its receptor (VGR). We investigated the expression and localization of VTG and VGR during gonad development of

Pampus

argenteus

. The qPCR results show that

vtgs

were not expressed in male fish

but in the ovary and liver of female fish; the expression levels went up at first and then down. The expression levels of

vgr

in the testis were low and only 1%–3% of that in ovary. ELISA results show that during the ovarian development of

argenteus

VTG in liver

serum

and ovary all showed a trend from increasing to decreasing. However

VTG in liver peaked in Stage Ⅳ

and in serum and ovary peaked in Stage V

reflecting changes in the characteristics of VTG in the liver (synthesis)

blood (transport)

and ovaries (accumulation). During gonad development

VGR in the ovaries first increased and then decreased

reaching a peak in Stage V

in contrast to

vgr

mRNA expression. The VGR content in the testis was extremely low and stable

consistent with

vgr

mRNA. Immunohistochemistry results show that the location and intensity of VTG and VGR positive signals were synchronized with the changes of their protein content

which rev

ealed that VTG was mainly synthesized in the liver cytoplasm

secreted into the blood

and transported to ovary in Stage Ⅲ. VGR is highly expressed in oocytes in Stage Ⅱ. In Stage Ⅲ

a large amount of VTG reaches the ovary

when VGR begins to translate and is subsequently transported to the plasma membrane of the oocyte. Therefore

the positive signal of VGR was stronger near the plasma membrane of oocytes in Stages Ⅰ and Ⅱ. By using qPCR

ELISA

and immunohistochemistry

the synthesis

transport

and accumulation of vitellogenin were elucidated and the mechanism of its endocytosis on egg membrane mediated by VTG during the development of

argenteus

was revealed preliminarily.

关键词

Keywords

references

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Expression and localization of vitellogenin genes (VTG) and receptor (VGR) in the gonad development of silver pomfret Pampus argenteus

Expression and localization of vitellogenin genes (VTG) and receptor (VGR) in the gonad development of silver pomfret Pampus argenteus

DOI：

摘要

Abstract

关键词

Keywords

references