Oyster studies reveal the duplication and functional diversification of Bivalvia caspase-8 genes

Shaoxi DENG; Tao QU; Guofan ZHANG; Fei XU

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Oyster studies reveal the duplication and functional diversification of Bivalvia caspase-8 genes

Biology | Updated：2024-10-12

- Oyster studies reveal the duplication and functional diversification of Bivalvia caspase-8 genes
- Oyster studies reveal the duplication and functional diversification of Bivalvia caspase-8 genes
- 海洋湖沼学报(英文) 2023年41卷第3期页码：1109-1121
- Affiliations：
  
  1. CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
  2. University of Chinese Academy of Sciences, Beijing 100049, China
  3. Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266000, China
  4. NHC Key Laboratory of Diagnosis and Therapy of Gastrointestinal Tumor, Gansu Provincial Hospital, Lanzhou 730000, China
- Author bio：
  
  xufei@qdio.ac.cn
- Funds：
  
  the Science & Technology Innovation Project of Laoshan Laboratory(LSKJ202203001);the Center for Ocean Mega-Research of Science, Chinese Academy of Sciences(COMS2019Q11);the GHfund B(20210702);the Taishan Scholars Program
- DOI：
  中图分类号：
- 收稿：2022-03-20，
  
  纸质出版：2023-05-01
- Accepted：
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Oyster studies reveal the duplication and functional diversification of Bivalvia caspase-8 genes[J]. 海洋湖沼学报(英文), 2023,41(3):1109-1121.

DENG Shaoxi,QU Tao,ZHANG Guofan,et al.Oyster studies reveal the duplication and functional diversification of Bivalvia caspase-8 genes[J].Journal of Oceanology and Limnology,2023,41(03):1109-1121.
Oyster studies reveal the duplication and functional diversification of Bivalvia caspase-8 genes[J]. 海洋湖沼学报(英文), 2023,41(3):1109-1121. DOI：

DENG Shaoxi,QU Tao,ZHANG Guofan,et al.Oyster studies reveal the duplication and functional diversification of Bivalvia caspase-8 genes[J].Journal of Oceanology and Limnology,2023,41(03):1109-1121. DOI：

摘要

Abstract

Caspase-8

first classified as a pro-apoptotic caspase

is considered to have arisen from duplication with caspase-10 and involves multiple immune and inflammatory responses in mammals. However

few are known on the phylogeny and function of caspase-8 in molluscs

one of the largest phyla in marine invertebrates. In this study

we conducted phylogenetic and functional analysis on molluscan caspase-8-like genes. Results indicate that duplication occurred in molluscan caspase-8-like genes

resulting in at least two caspase-8 copies in some groups of bivalves. Additional studies in Pacific oyster

Crassostrea gigas

showed diff

erent spatio-temporal expression patterns and subcellular localizations of

CgCaspase

and

CgCaspase

. While no interaction was observed between CgCaspase-8-2 and CgFADD

the adaptor molecule in apoptosis

yeast two-hybrid and co-immunoprecipitation assays suggested the interaction between CgCaspase-8-1 and CgFADD

indicating its pro-apoptotic function. In addition

CgCaspase-8-1 showed interaction with the CARD domain of CgRIG-I. Together with two NF-κB subunits (Cgp105 and CgRel)

their transcripts were up-regulated in response to poly(I:C) stimuli

supporting the immune function of both pro- and anti-inflammation. The results provide insight into the evolution and functional diversification of Bivalvia caspase-8 genes.

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references

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