Assessment of growth performance and transcriptome analysis of mechanisms underlying slow growth in triploid grass carp

Zixuan E; Xiaoman HUANG; Yonglin MIAO; Hao YANG; Han WEN; Mingqing ZHANG; Shujia LIAO; Danqi LU; Yong ZHANG; Shuisheng LI

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Assessment of growth performance and transcriptome analysis of mechanisms underlying slow growth in triploid grass carp

Updated：2026-04-07

- Assessment of growth performance and transcriptome analysis of mechanisms underlying slow growth in triploid grass carp
- Assessment of growth performance and transcriptome analysis of mechanisms underlying slow growth in triploid grass carp
- 海洋湖沼学报(英文) 2026年页码：1-10
- Affiliations：
  
  1.State Key Laboratory of Biocontrol and School of Life Sciences, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of Important Economic Fish, Institute of Aquatic Economic Animals, Sun Yat-Sen University, Guangzhou 510275, China
  2.Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao Marine Science and Technology Center, Qingdao 266237, China
- Author bio：
  
  lsszy@mail.sysu.edu.cn
  lshuish@mail.sysu.edu.cn
- Funds：
  
  the Science and Technology Innovation 2030-Major Project of Agricultural Biological Breeding(2023ZD0405505)
- DOI：
  中图分类号：
- 收稿：2025-08-18，
  
  网络首发：2026-04-07，
- Accepted：
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Zixuan E, Xiaoman HUANG, Yonglin MIAO, 等. Assessment of growth performance and transcriptome analysis of mechanisms underlying slow growth in triploid grass carp[J/OL]. 海洋湖沼学报(英文), 2026,1-10.

E Zixuan,HUANG Xiaoman,MIAO Yonglin,et al.Assessment of growth performance and transcriptome analysis of mechanisms underlying slow growth in triploid grass carp[J].Journal of Oceanology and Limnology,
Zixuan E, Xiaoman HUANG, Yonglin MIAO, 等. Assessment of growth performance and transcriptome analysis of mechanisms underlying slow growth in triploid grass carp[J/OL]. 海洋湖沼学报(英文), 2026,1-10. DOI：

E Zixuan,HUANG Xiaoman,MIAO Yonglin,et al.Assessment of growth performance and transcriptome analysis of mechanisms underlying slow growth in triploid grass carp[J].Journal of Oceanology and Limnology, DOI：.

摘要

Abstract

Triploid induction has been widely used in aquaculture to increase somatic growth

induce reproductive sterility

and mitigate ecological risks from uncontrolled breeding. However

the differences in growth between triploids and diploids are species specific. Grass carp (

Ctenopharyngodon

idella

) is a crucial species in China’s freshwater aquaculture industry. A one-year growth trial was conducted

and the results reveal that triploids exhibited a slower growth rate than diploids did. Further analyses demonstrated that the number of erythrocytes and the size of their nuclei were significantly greater in triploids than in their diploid counterparts (

0.001)

which was accompanied by a reduced packed cell volume (PCV) in triploids (

0.05). Histological analysis

revealed that the triploid liver displayed features of metabolic stress. Liver transcriptome analysis identified 713 differentially expressed genes (DEGs)

which showed primarily enrichment in metabolic processes

including hexose

lipid

and monosaccharide metabolism. This analysis also revealed significant downregulation of the growth hormone/insulin-like growth factor (GH/IGF) axis (

ghrb

igf1

and

igfbp5a

) and downstream signaling pathways

namely

PI3K-AKT in triploids. The results of this study characterize the early growth performance of triploid grass carp and provide molecular insights into the growth retardation observed in triploid individuals.

关键词

Keywords

references

Aydın İ , Küçük E , Polat H et al . 2021 . Growth and feed conversion ratio of diploid and triploid induced juvenile turbot reared at different water temperatures. Aquaculture , 543 : 736981 , https://doi.org/10.1016/j.aquaculture.2021.736981 https://doi.org/10.1016/j.aquaculture.2021.736981 .

Benfey T J . 1999 . The physiology and behavior of triploid fishes . Reviews in Fisheries Science , 7 ( 1 ): 39 - 67 , https://doi.org/10.1080/10641269991319162 https://doi.org/10.1080/10641269991319162 .

Cassani J R , Caton W E . 1986 . Growth comparisons of diploid and triploid grass carp under varying conditions . The Progressive Fish-Culturist , 48 ( 3 ): 184 - 187 , https://doi.org/10.1577/1548-8640(1986)48<184:GCODAT>2.0.CO;2. https://doi.org/10.1577/1548-8640(1986)48<184:GCODAT>2.0.CO;2.

Cassani J R , Maloney D R , Allaire H P et al . 1990 . Problems associated with tetraploid induction and survival in grass carp, Ctenopharyngodon idella . Aquaculture , 88 ( 3-4 ): 273 - 284 , https://doi.org/10.1016/0044-8486(90)90154-F https://doi.org/10.1016/0044-8486(90)90154-F .

Chatchaiphan S , Srisapoome P , Kim J H et al . 2017 . De novo transcriptome characterization and growth-related gene expression profiling of diploid and triploid bighead catfish ( Clarias macrocephalus Günther , 1864 ). Marine Biotechnology , 19 ( 1 ): 36 - 48 , https://doi.org/10.1007/s10126-017-9730-3 https://doi.org/10.1007/s10126-017-9730-3 .

Christensen K A , Sakhrani D , Rondeau E B et al . 2019 . Effect of triploidy on liver gene expression in coho salmon ( Oncorhynchus kisutch ) under different metabolic states . BMC Genomics , 20 ( 1 ): 336 , https://doi.org/10.1186/s12864-019-5655-8 https://doi.org/10.1186/s12864-019-5655-8 .

Creasy K T , Mehta M B , Schneider C V et al . 2025 . Ppp 1 r 3 b is a metabolic switch that shifts hepatic energy storage from lipid to glycogen. Science Advances , 11 ( 20 ): ead o 3440 , https://doi.org/10.1126/sciadv.ado3440 https://doi.org/10.1126/sciadv.ado3440 .

Dai X Y , Zhang W , Zhuo Z J et al . 2015 . Neuroendocrine regulation of somatic growth in fishes . Science China Life Sciences , 58 ( 2 ): 137 - 147 , https://doi.org/10.1007/s11427-015-4805-8 https://doi.org/10.1007/s11427-015-4805-8 .

Dehkhoda F , Lee C M M , Medina J et al . 2018 . The growth hormone receptor: mechanism of receptor activation, cell signaling, and physiological aspects. Frontiers in Endocrinology , 9 : 35 , https://doi.org/10.3389/fendo.2018.00035 https://doi.org/10.3389/fendo.2018.00035 .

E Z X , Wen H , Tang Y S et al . 2025 . Induction of triploid grass carp ( Ctenopharyngodon idella ) and changes in embryonic transcriptome . Animals , 15 ( 15 ): 2165 , https://doi.org/10.3390/ani15152165 https://doi.org/10.3390/ani15152165 .

Fast A W , Pewnim T , Keawtabtim R et al . 1995 . Comparative growth of diploid and triploid Asian catfish Clarias macrocephalus in Thailand . Journal of the World Aquaculture Society , 26 ( 4 ): 390 - 395 , https://doi.org/10.1111/j.1749-7345.1995.tb00834.x https://doi.org/10.1111/j.1749-7345.1995.tb00834.x .

Felip A , Piferrer F , Zanuy S et al . 2001 . Comparative growth performance of diploid and triploid European sea bass over the first four spawning seasons . Journal of Fish Biology , 58 ( 1 ): 76 - 88 , https://doi.org/10.1006/jfbi.2000.1427 https://doi.org/10.1006/jfbi.2000.1427 .

Ferguson R A , Tufts B L , Boutilier R G . 1989 . Energy metabolism in trout red cells: consequences of adrenergic stimulation in vivo and in vitro . The Journal of Experimental Biology , 143 ( 1 ): 133 - 147 , https://doi.org/10.1242/jeb.143.1.133 https://doi.org/10.1242/jeb.143.1.133 .

Guo X M , Hershberger W K , Myers J M . 1990 . Growth and survival of intrastrain and interstrain rainbow trout ( Oncorhynchus mykiss ) triploids . Journal of the World Aquaculture Society , 21 ( 4 ): 250 - 256 , https://doi.org/10.1111/j.1749-7345.1990.tb00536.x https://doi.org/10.1111/j.1749-7345.1990.tb00536.x .

Helms C C , Gladwin M T , Kim-Shapiro D B . 2018 . Erythrocytes and vascular function: oxygen and nitric oxide. Frontiers in Physiology , 9 : 125 , https://doi.org/10.3389/fphys.2018.00125 https://doi.org/10.3389/fphys.2018.00125 .

Jiang Y J , Xu W , Luo H R et al . 2024 . Hydrostatic pressure shock induced tetraploids in rice field eel ( Monopterus albus ). Aquaculture , 584 : 740636 , https://doi.org/10.1016/j.aquaculture.2024.740636 https://doi.org/10.1016/j.aquaculture.2024.740636 .

Kim D S , Jo J Y , Lee T Y . 1994 . Induction of triploidy in mud loach ( Misgurnus mizolepis ) and its effect on gonad development and growth . Aquaculture , 120 ( 3-4 ): 263 - 270 , https://doi.org/10.1016/0044-8486(94)90083-3 https://doi.org/10.1016/0044-8486(94)90083-3 .

Kimball S R , Shantz L M , Horetsky R L et al . 1999 . Leucine regulates translation of specific mRNAs in L6 myoblasts through mTOR-mediated changes in availability of eIF4E and phosphorylation of ribosomal protein S6 . Journal of Biological Chemistry , 274 ( 17 ): 11647 - 11652 , https://doi.org/10.1074/jbc.274.17.11647 https://doi.org/10.1074/jbc.274.17.11647 .

Li B , Dewey C N . 2011 . RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome . BMC Bioinformatics , 12 ( 1 ): 323 , https://doi.org/10.1186/1471-2105-12-323 https://doi.org/10.1186/1471-2105-12-323 .

Li L S , Balto G , Xu X Y et al . 2023 . The feeding ecology of grass carp: a review . Reviews in Aquaculture , 15 ( 4 ): 1335 - 1354 , https://doi.org/10.1111/raq.12777 https://doi.org/10.1111/raq.12777 .

Li S F , Zou S M , Cai W Q et al . 2006 . Production of interploid triploids by 4n x 2n blunt snout bream ( Megalobrama amblycephala . Yih) and their first performance data . Aquaculture Research , 37 ( 4 ): 374 - 379 , https://doi.org/10.1111/j.1365-2109.2006.01441.x https://doi.org/10.1111/j.1365-2109.2006.01441.x .

Love M I , Huber W , Anders S . 2014 . Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2 . Genome Biology , 15 ( 12 ): 550 , https://doi.org/10.1186/s13059-014-0550-8 https://doi.org/10.1186/s13059-014-0550-8 .

Odei D K , Hagen Ø , Peruzzi S et al . 2020 . Transcriptome sequencing and histology reveal dosage compensation in the liver of triploid pre-smolt Atlantic salmon . Scientific Reports , 10 ( 1 ): 16836 , https://doi.org/10.1038/s41598-020-73814-6 https://doi.org/10.1038/s41598-020-73814-6 .

Oppedal F , Taranger G L , Hansen T . 2003 . Growth performance and sexual maturation in diploid and triploid Atlantic salmon ( Salmo salar L.) in seawater tanks exposed to continuous light or simulated natural photoperiod . Aquaculture , 215 ( 1-4 ): 145 - 162 , https://doi.org/10.1016/S0044-8486(02)00223-5 https://doi.org/10.1016/S0044-8486(02)00223-5 .

Ren L , Tang C C , Li W H et al . 2017 . Determination of dosage compensation and comparison of gene expression in a triploid hybrid fish . BMC Genomics , 18 ( 1 ): 38 , https://doi.org/10.1186/s12864-016-3424-5 https://doi.org/10.1186/s12864-016-3424-5 .

Roques S , Deborde C , Richard N et al . 2020 . Metabolomics and fish nutrition: a review in the context of sustainable feed development . Reviews in Aquaculture , 12 ( 1 ): 261 - 282 , https://doi.org/10.1111/raq.12316 https://doi.org/10.1111/raq.12316 .

Sheridan M A . 2021 . Coordinate regulation of feeding, metabolism, and growth: Perspectives from studies in fish. General and Comparative Endocrinology , 312 : 113873 , https://doi.org/10.1016/j.ygcen.2021.113873 https://doi.org/10.1016/j.ygcen.2021.113873 .

Shu H , Yang L D , Li Y et al . 2015 . Comparative study on haematology and physiology of Epinephelus coioides (♀)× Epinephelus lanceolatus (♂) hybrid F1 Qinglong grouper . Oceanologia et Limnologia Sinica , 46 ( 2 ): 396 - 402 , https://doi.org/10.11693/hyhz20140900244. https://doi.org/10.11693/hyhz20140900244. (in Chinese with English abstract)

Soltan M A , AbouZead M Y , Mohamed W E . 2017 . Growth performance, feed utilization and gonad development of diploid and triploid Nile tilapia, Oreochromis niloticus . Egyptian Journal of Nutrition and Feeds , 20 ( 2 ): 297 - 305 , https://doi.org/10.21608/ejnf.2017.104137 https://doi.org/10.21608/ejnf.2017.104137 .

Stitt T N , Drujan D , Clarke B A et al . 2004 . The IGF-1/PI3K/Akt pathway prevents expression of muscle atrophy-induced ubiquitin ligases by inhibiting FOXO transcription factors . Molecular Cell , 14 ( 3 ): 395 - 403 , https://doi.org/10.1016/s1097-2765(04)00211-4 https://doi.org/10.1016/s1097-2765(04)00211-4 .

Tiwary B K , Kirubagaran R , Ray A K . 2004 . The biology of triploid fish . Reviews in Fish Biology and Fisheries , 14 ( 4 ): 391 - 402 , https://doi.org/10.1007/s11160-004-8361-8 https://doi.org/10.1007/s11160-004-8361-8 .

Xu J H , You F , Wu X F et al . 2008 . Induction of triploidy in large yellow crocker Pseudosciaena crocea (Richardson , 1846 ): effects of pressure shocks and growth performance in the first rearing year. Aquaculture Research , 39 ( 13 ): 1369 - 1376 , https://doi.org/10.1111/j.1365-2109.2008.02005.x https://doi.org/10.1111/j.1365-2109.2008.02005.x .

Zou S M , Li S F , Cai W Q et al . 2005 . Characters of karyotype and morphology in auto-4n, 4n-F1 and reciprocal interploid 3n cross of blunt snout bream Megalobrama amblycephala . Acta Zoologica Sinica , 51 ( 3 ): 455 - 461 . (in Chinese with English abstract)

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