Enhancing reproductive efficiency of mud crab <italic style="font-style: italic">Scylla</italic> <italic style="font-style: italic">paramamosain</italic> in RAS: effects of temperature and eyestalk ablation on ovarian development, lipid metabolism, and spawning rate

Kaijia YU; Ce SHI; Chunlin WANG; Ramón SALCEDO; Ingrid MASALÓ; Changkao MU; Shujian CHEN

doi:10.1007/s00343-025-5340-5

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Enhancing reproductive efficiency of mud crab Scylla paramamosain in RAS: effects of temperature and eyestalk ablation on ovarian development, lipid metabolism, and spawning rate

Aquaculture and Fisheries | Updated：2026-07-09

- Enhancing reproductive efficiency of mud crab Scylla paramamosain in RAS: effects of temperature and eyestalk ablation on ovarian development, lipid metabolism, and spawning rate
- Journal of Oceanology and Limnology Vol. 44, Pages: 1789-1807(2026)
- Affiliations：
  
  1.Key Laboratory of Aquacultural Biotechnology, Ningbo University, Ministry of Education, Ningbo 315832, China
  2.Key Laboratory of Green Mariculture (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Ningbo 315832, China
  3.Research Academy of Sanmen Mud Crab Industrial Technology, Taizhou 317109, China
  4.Department of Agri-Food Engineering and Biotechnology, Universitat Politècnica de Catalunya-Barcelona TECH, Castelldefels 08860, Spain
- Author bio：
  
  shice3210@126.com
- Funds：
- DOI：10.1007/s00343-025-5340-5
  CLC：
- Received：09 September 2025，
  
  Accepted：20 October 2025，
  
  Online First：09 July 2026，
  
  Published：01 July 2026
- Accepted：
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YU Kaijia,SHI Ce,WANG Chunlin,et al.Enhancing reproductive efficiency of mud crab ,Scylla ,paramamosain in RAS: effects of temperature and eyestalk ablation on ovarian development, lipid metabolism, and spawning rate[J].Journal of Oceanology and Limnology,
DOI：

YU Kaijia,SHI Ce,WANG Chunlin,et al.Enhancing reproductive efficiency of mud crab ,Scylla ,paramamosain in RAS: effects of temperature and eyestalk ablation on ovarian development, lipid metabolism, and spawning rate[J].Journal of Oceanology and Limnology, DOI：.

摘要

Abstract

A 12-week experimental study was conducted to explore the influence of temperature and eyestalk ablation on the spawning rate and ovarian development of mud crab

Scylla

paramamosain

. The study comprised four experimental treatments: 25 °C (coded 25)

25 °C with eyestalk ablation (25EA)

30 °C (30)

and 30 °C with eyestalk ablation (30EA). Results indicate that nei

ther temperature nor eyestalk ablation significantly influenced the survival rate

HSI

or GSI (

0.05). Nevertheless

eyestalk ablation significantly enhanced the spawning rate and oocyte diameter

with the 30EA group showing the greatest increase (

0.05). Hormonal analysis of the hemolymph showed that eyestalk ablation significantly decreased gonad-inhibiting hormone (GIH) levels while elevating gonad-stimulating hormone (GTH) levels (

0.05). Under 30 °C with eyestalk ablation

significant elevations in triglyceride (TG) levels were observed in the hemolymph

ovary

and hepatopancreas (

0.05). Furthermore

eyestalk ablation significantly elevated vitellogenin (VTG) contents in both hepatopancreas and hemolymph. Besides

eyestalk ablation induced a significant upregulation of lipid synthesis (

fas

6pgd

) and lipid transport (

fatp

) genes in the hepatopancreas (

0.05). Similarly

elevated temperature significantly induced the upregulation of lipid synthesis (

fas

6pgd

)

lipid catabolism (

hsl

aco

)

and lipid transport (

fatp

fabp

) genes in the ovary (

0.05). Additionally

eyestalk ablation considerably enhanced the expression of

fatp

in the ovary (

0.05). Therefore

eyestalk ablation enhanced lipid accumulation in the ovary by reducing GIH levels

leading to increased oocyte diameter and ultimately enhancing the spawning rate of the crabs. In conclusion

eyestalk ablation significantly improved the spawning rate of

paramamosain

under temperatures of 25–30 °C. These findings provide theoretical support for optimizing artificial breeding strategies and improving seed production efficiency in mud

crab aquaculture.

关键词

Keywords

references

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Enhancing reproductive efficiency of mud crab Scylla paramamosain in RAS: effects of temperature and eyestalk ablation on ovarian development, lipid metabolism, and spawning rate

DOI：10.1007/s00343-025-5340-5

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