Physiological and molecular responses to sodium acetate addition and supplementation in the two stages of <italic style="font-style: italic">Haematococcus</italic> <italic style="font-style: italic">pluvialis</italic>

Yanbin LI; Weizhao HAN; Na LI; Tian XIE; Xin TAN; Song TANG; Yequan SHENG; Bangxiang HE

doi:10.1007/s00343-025-5061-9

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Physiological and molecular responses to sodium acetate addition and supplementation in the two stages of Haematococcus pluvialis

Biology | Updated：2026-05-07

- Physiological and molecular responses to sodium acetate addition and supplementation in the two stages of Haematococcus pluvialis
- Journal of Oceanology and Limnology Vol. 44, Issue 2, Pages: 768-783(2026)
- Affiliations：
  
  1.College of Biological and Food Engineering, Anhui Polytechnic University, Wuhu 241000, China
  2.Anhui Engineering Laboratory for Industrial Microbiology Molecular Breeding, Anhui Polytechnic University, Wuhu 241000, China
- Author bio：
  
  hbxwork@ahpu.edu.cn
- Funds：
- DOI：10.1007/s00343-025-5061-9
  CLC：
- Received：04 March 2025，
  
  Accepted：07 April 2025，
  
  Online First：25 June 2025，
  
  Published：01 March 2026
- Accepted：
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LI Yanbin,HAN Weizhao,LI Na,et al.Physiological and molecular responses to sodium acetate addition and supplementation in the two stages of ,Haematococcus ,pluvialis[J].Journal of Oceanology and Limnology,2026,44(02):768-783.
DOI：

LI Yanbin,HAN Weizhao,LI Na,et al.Physiological and molecular responses to sodium acetate addition and supplementation in the two stages of ,Haematococcus ,pluvialis[J].Journal of Oceanology and Limnology,2026,44(02):768-783. DOI： 10.1007/s00343-025-5061-9.

摘要

Abstract

The effects and mechanism of NaAc addition and supply modes growth in both green and red stages of

Haematococcus

pluvialis

were investigated

and 1-g/L NaAc was found the optimal initial concentration for the two stages. On this basis

the extra supplement of 1-g/L NaAc on Day 6 of the green stage or on Day 3 of the red stage could further increase the accumulation of biomass. Compared with autotrophic control

the optimal NaAc supply mode increased the dry weight by 135.71% (green stage) and 91.11% (red stage)

while astaxanthin and lipid were increased by 56.9% and 42.86% in red stage

respectively. Gene expression detection revealed that acetyl-CoA synthetase (

acs

)

acs2

acs3

and

acs5

were primarily responsible for the NaAc uptake during the green stage

and

acs4

played a critical role in stress response during the red stage

additionally

key genes associated with acetate metabolism

astaxanthin synthesis

and lipid synthesis

were sign

ificantly upregulated under optimal NaAc supply mode. The findings provide practical and theoretical insights for NaAc use in

Haematococcus

pluvialis

关键词

Keywords

references

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Related Institution

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College of Ocean and Earth Sciences, Xiamen University

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⁰

Physiological and molecular responses to sodium acetate addition and supplementation in the two stages of Haematococcus pluvialis

DOI：10.1007/s00343-025-5061-9

摘要

Abstract

关键词

Keywords

references