Transcriptomic insights into the adaptive mechanisms of antennal glands in mud crab (<italic style="font-style: italic">Scylla paramamosain</italic>) to acute chloride-type low-salinity alkaline water stress

Jinwei WANG; Xiaosong JIANG; Changkao MU; Chunlin WANG; Huan WANG

doi:10.1007/s00343-025-4331-x

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Transcriptomic insights into the adaptive mechanisms of antennal glands in mud crab (Scylla paramamosain) to acute chloride-type low-salinity alkaline water stress

Biology | Updated：2025-12-16

- Transcriptomic insights into the adaptive mechanisms of antennal glands in mud crab (Scylla paramamosain) to acute chloride-type low-salinity alkaline water stress
- Journal of Oceanology and Limnology Vol. 43, Issue 6, Pages: 1938-1950(2025)
- Affiliations：
  
  1.School of Marine Science, Ningbo University, Ningbo 315832, China
  2.Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang 524013, China
  3.Key Laboratory of Aquacultral Biotechnology Ministry of Education, Ningbo University, Ningbo 315832, China
- Author bio：
  
  wangchunlin@nbu.edu.cn
  wanghuan1@nbu.edu.cn
- Funds：
- DOI：10.1007/s00343-025-4331-x
  CLC：
- Received：06 December 2024，
  
  Published：01 November 2025
- Accepted：
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WANG Jinwei,JIANG Xiaosong,MU Changkao,et al.Transcriptomic insights into the adaptive mechanisms of antennal glands in mud crab (,Scylla paramamosain) to acute chloride-type low-salinity alkaline water stress[J].Journal of Oceanology and Limnology,2025,43(06):1938-1950.
DOI：

WANG Jinwei,JIANG Xiaosong,MU Changkao,et al.Transcriptomic insights into the adaptive mechanisms of antennal glands in mud crab (,Scylla paramamosain) to acute chloride-type low-salinity alkaline water stress[J].Journal of Oceanology and Limnology,2025,43(06):1938-1950. DOI： 10.1007/s00343-025-4331-x.

摘要

Abstract

The mud crab (

Scylla

paramamosain

) has been successfully cultivated in chloride-type low-salinity alkaline waters

yet the molecular mechanisms underlying its adaptation to this environment remain elusive. This study seeks to uncover the adaptation mechanisms of the antennal gland of mud crab under acute chloride-type low-salinity alkaline water stress. Three-hundred crabs were randomly selected and divided into two groups: the low salinity (LS

salinity of 2.2) group and the control group (CK

salinity of 23.4). Following a 120-h experimental period

the antennal glands were sampled for transcriptomic analysis. Experimental results show that acute chloride-type low-salinity saline-alkaline water stress led to a significant decline in the survivorship of mud crab. Through comparative transcriptomic analysis

we identified 923 differentially expressed genes (DEGs)

among which 272 were upregulated and 651 were downregulated. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that the majority of these DEGs are closely linked to functions such as energy metabolism and osmoregulation. Notably

the pathways involving oxidoreductase activity

oxidative phosphorylation

symporter a

ctivity

and intracellular calcium-activated chloride channels were significantly enriched

highlighting their pivotal roles in enhancing the osmoregulatory capacity of mud crab and maintaining internal homeostasis in chloride-type low-salinity alkaline environments. Additionally

the upregulation of genes such as

SLC6A9

SLC6A4

and

further facilitated transmembrane ion transport and energy metabolism

thereby reinforcing intracellular isosmotic regulation. However

these controls also pose a potential risk of oxidative stress. Therefore

the antennal gland plays a crucial role in the response of mud crab to acute chloride-type low-salinity alkaline water stress. This study provides new insights into the adaptive mechanisms of mud crab and lays a theoretical foundation for enhancing chloride-type low-salinity alkaline water aquaculture techniques.

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Keywords

references

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Chunlin WANG

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Related Institution

School of Marine Science and Engineering, Qingdao Agricultural University

State Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Oceanology, Chinese Academy of Sciences

Laboratory for Marine Biology and Biotechnology, Qingdao Marine Science and Technology Center

Marine Sciences Research Institute of Shandong Province, National Oceanographic Center

College of Marine Science, University of Chinese Academy of Sciences

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Transcriptomic insights into the adaptive mechanisms of antennal glands in mud crab (Scylla paramamosain) to acute chloride-type low-salinity alkaline water stress

DOI：10.1007/s00343-025-4331-x

摘要

Abstract

关键词

Keywords

references