VP39A is a novel host cellular binding protein of white spot syndrome virus and interacts with Na<sup>+</sup>, K<sup>+</sup>-ATPase alpha subunit

Zengzhi YUAN; Wei OUYANG; Xinyi JIANG; Dan ZOU; Xuhui KONG; Jinsheng SUN

doi:10.1007/s00343-025-4324-9

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VP39A is a novel host cellular binding protein of white spot syndrome virus and interacts with Na⁺, K⁺-ATPase alpha subunit

Aquaculture and Fisheries | Updated：2026-05-07

- VP39A is a novel host cellular binding protein of white spot syndrome virus and interacts with Na⁺, K⁺-ATPase alpha subunit
- Journal of Oceanology and Limnology Vol. 44, Issue 2, Pages: 890-903(2026)
- Affiliations：
  
  1.Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin 300387, China
  2.College of Life Sciences, Tianjin Normal University, Tianjin 300387, China
- Author bio：
  
  skysjs@tjnu.edu.cn
- Funds：
- DOI：10.1007/s00343-025-4324-9
  CLC：
- Received：03 December 2024，
  
  Accepted：03 March 2025，
  
  Online First：16 May 2025，
  
  Published：01 March 2026
- Accepted：
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YUAN Zengzhi,OUYANG Wei,JIANG Xinyi,et al.VP39A is a novel host cellular binding protein of white spot syndrome virus and interacts with Na,⁺, K,⁺-ATPase alpha subunit[J].Journal of Oceanology and Limnology,2026,44(02):890-903.
DOI：

YUAN Zengzhi,OUYANG Wei,JIANG Xinyi,et al.VP39A is a novel host cellular binding protein of white spot syndrome virus and interacts with Na,⁺, K,⁺-ATPase alpha subunit[J].Journal of Oceanology and Limnology,2026,44(02):890-903. DOI： 10.1007/s00343-025-4324-9.

摘要

Abstract

White spot syndrome virus (WSSV) is one of the most virulent pathogens in shrimp aquaculture. An in-depth understanding of the surface protein-protein interplays between WSSV and host is the key to further elucidating the pathogenesis mechanism. In this study

VP39A was identified as a novel cellular binding protein of WSSV by reversely conducting the biotinylation based affinity chromatography (BBAC) method. The result of indirect immunofluorescence assay

competitive ELISA

and in-vivo neutralization assays show that VP39A is vital to the cellular entry and infection of WSSV. Na

-ATPase alpha subunit (NKAα)

a trans-membrane subunit of complex Na

-ATPase

was captured as the VP39A binding protein with pull-down assay. Co-localization of NKAα with VP39A in shrimp hemocytes was confirmed by immunofluorescence and co-immunoprecipitation studies. In addition

blocking with anti-NKAα antibody can reduce the binding of WSSV to the gill cellular membrane proteins. These results collectively demonstrate that VP39A plays an important role in the viral adsorption via its interaction with NKAα

which could be an effective target for drug design.

关键词

Keywords

references

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Qingdao National Laboratory for Marine Science and Technology

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VP39A is a novel host cellular binding protein of white spot syndrome virus and interacts with Na+, K+-ATPase alpha subunit

DOI：10.1007/s00343-025-4324-9

摘要

Abstract

关键词

Keywords

references

VP39A is a novel host cellular binding protein of white spot syndrome virus and interacts with Na⁺, K⁺-ATPase alpha subunit