Regulation of ammonia stress adaptation in <italic style="font-style: italic">Penaeus</italic> <italic style="font-style: italic">vannamei</italic> gills: responses of endoplasmic reticulum stress and Ca<sup>2+</sup> homeostasis

Wenheng LI; Changjian LI; Fang WANG; Zhitao HUANG; Hongwei SHAN

doi:10.1007/s00343-025-4279-x

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Regulation of ammonia stress adaptation in Penaeus vannamei gills: responses of endoplasmic reticulum stress and Ca²⁺ homeostasis

Biology | Updated：2025-12-16

- Regulation of ammonia stress adaptation in Penaeus vannamei gills: responses of endoplasmic reticulum stress and Ca²⁺ homeostasis
- Journal of Oceanology and Limnology Vol. 43, Issue 6, Pages: 1951-1961(2025)
- Affiliations：
  
  1.Key Laboratory of Mariculture (Ocean University of China), Ministry of Education, Qingdao 266003, China
  2.Lianyungang Marine and Fishery Development Promotion Center, Lianyungang 222000, China
  3.Norwegian Institute for Water Research (NIVA), Bergen 5006, Norway
- Author bio：
  
  shanhongwei@ouc.edu.cn
- Funds：
- DOI：10.1007/s00343-025-4279-x
  CLC：
- Received：01 December 2024，
  
  Online First：27 February 2025，
  
  Published：01 November 2025
- Accepted：
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LI Wenheng,LI Changjian,WANG Fang,et al.Regulation of ammonia stress adaptation in ,Penaeus ,vannamei gills: responses of endoplasmic reticulum stress and Ca,²⁺ homeostasis[J].Journal of Oceanology and Limnology,2025,43(06):1951-1961.
DOI：

LI Wenheng,LI Changjian,WANG Fang,et al.Regulation of ammonia stress adaptation in ,Penaeus ,vannamei gills: responses of endoplasmic reticulum stress and Ca,²⁺ homeostasis[J].Journal of Oceanology and Limnology,2025,43(06):1951-1961. DOI： 10.1007/s00343-025-4279-x.

摘要

Abstract

Endoplasmic reticulum (ER) stress and Ca

homeostasis play important roles in the environmental adaptation of aquatic animals. To elucidate the response and correlation of ER stress and Ca

homeostasis in the gills of

Penaeus

vannamei

under ammonia stress

we investigated the expression levels of genes related to ER stress and Ca

homeostasis in the gills of

vannamei

exposed to 0- (control)

3.8-

7.6-

and 11.3-mg/L total ammonia nitrogen for 96 h. Results show that the relative gene expression levels of the ER stress indicators

Bip

eiF2α

ATF4

IRE1

and

XBP1s

were higher in the stress groups than in the control group at 12 and 48 h

reaching the maximum at 48 h (

0.05). However

at 96 h

the levels of these indicators were lower in the 7.6- and 11.3-mg/L groups than in the control group. The relative expression levels of

SERCA

RyR

MCU

and

Letm

were significantly higher in the stress groups than in the control group at 12

and 48 h (

0.05)

and the expression levels were positively correlated with the ammonia stress concentration. At 96 h

the expression levels of

SERCA

in the stress groups were lower than that in the control group

whereas the expression levels of

RyR

in the stress groups were significantly higher than that in the control group (

0.05). The relative expression levels of

MCU

were not significantly different fr

om those in the control group. The relative expression levels of

in the 7.6- and 11.3-mg/L groups were significantly lower than that in the control group during the ammonia stress period (

0.05). Pearson’s correlation analysis showed significant correlations among NH

-N levels

the expression of ER stress indicator genes

and the expression of genes related to the regulation of Ca

homeostasis. The above results indicate that ammonia stress could induce ER stress

and consequently changed the mitochondrial and ER Ca

homeostasis in the gills of

vannamei

which contribute to its adaptive mechanisms under adverse environmental conditions.

关键词

Keywords

references

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Related Institution

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⁰

Regulation of ammonia stress adaptation in Penaeus vannamei gills: responses of endoplasmic reticulum stress and Ca2+ homeostasis

DOI：10.1007/s00343-025-4279-x

摘要

Abstract

关键词

Keywords

references

Regulation of ammonia stress adaptation in Penaeus vannamei gills: responses of endoplasmic reticulum stress and Ca²⁺ homeostasis