Transcriptomic dynamics in the hepatopancreas of <italic style="font-style: italic">Macrobrachium</italic> <italic style="font-style: italic">rosenbergii</italic> during thermal elimination of infection with Decapod Iridescent Virus 1

Xingyu ZHENG; Miaoying CAI; Qi SHEN; Liping LI; Zhenglong XIA; Quanxin GAO; Qiongying TANG; Guoliang YANG; Jiongying YU; Shaokui YI

doi:10.1007/s00343-025-4256-4

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Transcriptomic dynamics in the hepatopancreas of Macrobrachium rosenbergii during thermal elimination of infection with Decapod Iridescent Virus 1

Aquaculture and Fisheries | Updated：2026-03-26

- Transcriptomic dynamics in the hepatopancreas of Macrobrachium rosenbergii during thermal elimination of infection with Decapod Iridescent Virus 1
- Journal of Oceanology and Limnology Vol. 44, Issue 1, Pages: 445-459(2026)
- Affiliations：
  
  1.School of Life Sciences, Huzhou University, Huzhou 313000, China
  2.Jiangsu Shufeng Prawn Breeding Co., Ltd., Gaoyou 225654, China
- Author bio：
  
  yujiongying@outlook.com
  yishaokui@foxmail.com
- Funds：
- DOI：10.1007/s00343-025-4256-4
  CLC：
- Received：24 September 2024，
  
  Accepted：09 January 2025，
  
  Online First：14 May 2025，
  
  Published：01 January 2026
- Accepted：
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ZHENG Xingyu,CAI Miaoying,SHEN Qi,et al.Transcriptomic dynamics in the hepatopancreas of Macrobrachium rosenbergii during thermal elimination of infection with Decapod Iridescent Virus 1[J].Journal of Oceanology and Limnology,2026,44(01):445-459.
DOI：

ZHENG Xingyu,CAI Miaoying,SHEN Qi,et al.Transcriptomic dynamics in the hepatopancreas of Macrobrachium rosenbergii during thermal elimination of infection with Decapod Iridescent Virus 1[J].Journal of Oceanology and Limnology,2026,44(01):445-459. DOI： 10.1007/s00343-025-4256-4.

摘要

Abstract

Decapod Iridescent Virus 1 (DIV1) is a recently discovered virus recognized for its high infectivity in

Macrobrachium

rosenbergii

. A thermal treatment was performed on DIV1-infected

rosenbergii

and the therapeutic efficacy was evaluated. In the DIV1 challenge experiment

the mortality rate in the challenged group was found to be 2.6 times greater than that in the control group

with the vir

al load in deceased individuals exceeding 5.41×10

copies/μg-DNA. The thermal treatment (TT) was administered at 36 °C for a duration of 16 d

followed by a temperature restoration (TR) period at 26 °C for 3 d. On the first day at 36 °C

an average viral concentration of 5.34×10 copies/μg-DNA

was detected in the survived individuals. RNA-seq analysis showed a significant upregulation of genes related to the lysosome pathway

including sialin-like isoform x2 (

slc17a5

)

beta-galactosidase-1-like protein 2 (

glb1

)

putative glucosylceramidase 3 (

gba

)

sphingomyelin phosphodiesterase-like isoform x2 (

smpd1

)

beta-hexosaminidase subunit alpha-like (

hexa_b

) and legumain-like protein (

lgmn

)

following a transient suppression period induced by thermal stress. Upon reaching 36 °C

the activation of heat shock protein 70 (

hsp70

) and heat shock protein 90 (

hsp90a

) was observed. Concomitantly

genes that implicated in energy production critical for DIV1 replication

such as hexokinase (

) and microsomal glutathione s-transferase 3-like isoform x2 (

gst

)

were inhibited. These results collectively suggest that TT/TR treatments eliminated DIV1 in

rosenbergii

by activating the organism’s innate immune response and suppressing virus replication. This study provides a theoretical basis for utilizing thermal therapy in the management of viral infections in

rosenbergii

breeding programs

thereby facilitating the development of new strains resistant to DIV1.

关键词

Keywords

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Related Author

Guoliang YANG

Zhenglong XIA

Miaoying CAI

Jinping LUO

Xin PENG

Haihui TU

Xinyi YAO

Xuan LAN

Related Institution

Jiangsu Shufeng Prawn Breeding Co. Ltd., Gaoyou

Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, College of Life Sciences, Huzhou University

Fengjiashan Reservoir Administration Bureau

School of Marine Sciences, Ningbo University

Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development; Key Laboratory of Aquatic Animal Genetic Breeding and Nutrition, Chinese Academy of Fishery Sciences; College of Life Sciences, Huzhou University

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Transcriptomic dynamics in the hepatopancreas of Macrobrachium rosenbergii during thermal elimination of infection with Decapod Iridescent Virus 1

DOI：10.1007/s00343-025-4256-4

摘要

Abstract

关键词

Keywords

references