Toxicity testing of <italic style="font-style: italic">Ostreopsis</italic> cf. <italic style="font-style: italic">ovata</italic>: mouse bioassay, cytotoxicity, and hemolysis neutralization assay

Yuping LAI; Qingchun XU; Wenyu GAN; Hanbai WANG; Shiwei KOU; Tianjiu JIANG; Tao JIANG

doi:10.1007/s00343-025-4250-x

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Toxicity testing of Ostreopsis cf. ovata: mouse bioassay, cytotoxicity, and hemolysis neutralization assay

Ecology | Updated：2025-12-16

- Toxicity testing of Ostreopsis cf. ovata: mouse bioassay, cytotoxicity, and hemolysis neutralization assay
- Journal of Oceanology and Limnology Vol. 43, Issue 6, Pages: 1902-1912(2025)
- Affiliations：
  
  1.Research Center of Harmful Algae & Marine Biology, Jinan University, Guangzhou 510632, China
  2.School of Ocean, Yantai University, Yantai 264005, China
  3.Linyi Agricultural Development Group Co., Ltd., Linyi 276002, China
- Author bio：
  
  tjiangtj@jnu.edu.cn
  jiangtaophy@163.com
- Funds：
- DOI：10.1007/s00343-025-4250-x
  CLC：
- Received：21 September 2024，
  
  Online First：14 January 2025，
  
  Published：01 November 2025
- Accepted：
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LAI Yuping,XU Qingchun,GAN Wenyu,et al.Toxicity testing of Ostreopsis cf. ovata: mouse bioassay, cytotoxicity, and hemolysis neutralization assay[J].Journal of Oceanology and Limnology,2025,43(06):1902-1912.
DOI：

LAI Yuping,XU Qingchun,GAN Wenyu,et al.Toxicity testing of Ostreopsis cf. ovata: mouse bioassay, cytotoxicity, and hemolysis neutralization assay[J].Journal of Oceanology and Limnology,2025,43(06):1902-1912. DOI： 10.1007/s00343-025-4250-x.

摘要

Abstract

Ostreopsis

cf.

ovata

is a marine benthic dinoflagellate in tropical and temperate seas and can produce potent toxic compounds. The existence of

. cf.

ovata

has been found in the Chinese coastal areas

but studies on its toxicity are very few. This study investigated the toxicity of the

. cf.

ovata

(TIO991) isolated from Weizhou Island in the South China Sea by using methanol and chloroform to extract toxic compounds from the algal cells cultured indoor. Experiments on mouse acute toxicity showed that the crude methanol extract (CME) of

. cf.

ovata

caused the death of mice in 16–18 min. Furthermore

CME inhibited the cell reproduction of human neuroblastoma cells (BE (2)-M17 cells) by Cell Counting Kit-8 with a dose- and time-effect relationship and caused cell death in the form of cell necrosis. We found that CME had strong hemolytic activity and was significantly inhibited by ouabain

indicating that CME might contain palytoxins. By contrast

the crude chloroform extract of

. cf.

ovata

was relatively weak in toxicity as obtained in our experiments on mouse acute

toxicity

cytotoxicity

and hemolytic activity. This suggests that the algae may raise the potential threat to marine ecosystems and public health.

关键词

Keywords

references

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Toxicity testing of Ostreopsis cf. ovata: mouse bioassay, cytotoxicity, and hemolysis neutralization assay

DOI：10.1007/s00343-025-4250-x

摘要

Abstract

关键词

Keywords

references