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Development and application of a colloidal gold test strip for detection of antibodies against infectious spleen and kidney necrosis virus (ISKNV) in mandarin fish
Siniperca chuatsi - Journal of Oceanology and Limnology Vol. 43, Issue 6, Pages: 1974-1984(2025)
- Affiliations：
1.National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai 201306, China
2.Jiangsu Province Engineering Research Center for Marine Bio-resources Sustainable Utilization, College of Oceanography, Hohai University, Nanjing 210024, China
3.Key Laboratory of Agriculture Ministry for Freshwater Aquatic Genetic Resources, Shanghai Ocean University, Shanghai 201306, China
- Author bio：
lqlv@shou.edu.cn
- Funds：
DOI：10.1007/s00343-025-4239-5
CLC：Received：13 September 2024，
Published：01 November 2025
- Accepted：
Scan QR Code
JI Xiaohua,ZHANG Xiaomi,SUN Yuchong,et al.Development and application of a colloidal gold test strip for detection of antibodies against infectious spleen and kidney necrosis virus (ISKNV) in mandarin fish ,Siniperca ,chuatsi[J].Journal of Oceanology and Limnology,2025,43(06):1974-1984.
JI Xiaohua,ZHANG Xiaomi,SUN Yuchong,et al.Development and application of a colloidal gold test strip for detection of antibodies against infectious spleen and kidney necrosis virus (ISKNV) in mandarin fish ,Siniperca ,chuatsi[J].Journal of Oceanology and Limnology,2025,43(06):1974-1984. DOI： 10.1007/s00343-025-4239-5.
摘要
Abstract
Mass mortality of mandarin fish (
Siniperca
chuatsi
) due to infectious spleen and kidney necrosis virus (ISKNV) infection occurs frequently. Since there are no effective drug treatments available
prevention relies heavily on detection. Effective and rapid on-site detection methods are needed for early diagnosis of ISKNV. In this study
a rapid and simple colloidal gold test strip method
specific for the antibody against major capsid protein (MCP)
was developed and systematically evaluated for the detection of ISKNV. The limit of detection of the test strip is a 1꞉100 dilution of a positive standard serum and the antibody level in the fish could be estimated from the depth of color of the test line. The strips were tested against serum samples of cyprinid herpesvirus-2 (CyHV-2)
grass carp reovirus (GCRV)
largemouth bass ranavirus (LMBV)
large yellow croaker iridovirus (LYCIV)
and spring viremia of carp virus (SVCV)
yielding no cross-reactivity. In addition
10 mandarin fish artificially infected with ISKNV were tested using the current industry standard PCR method (SC/T 7211-2011) on their splenorenal tissues. The results from the test strips showed a high degree of c
oncordance with PCR testing
achieving a Kappa value of 0.737. All the results indicated that the colloidal gold test strips prepared in this study could be used as a simple
rapid
and highly sensitive and specific method for ISKNV diagnosis.
关键词
Keywords
references
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