Effect of the <italic style="font-style: italic">5</italic>-<italic style="font-style: italic">HT<sub>7Ar</sub></italic> gene on the reproductive performance of parthenogenetic <italic style="font-style: italic">Artemia</italic>

Rui ZHANG; Xuemei OUYANG; Ke LI; Chi ZHANG; Liangsen LIU; Liying SUI; Xuekai HAN

doi:10.1007/s00343-025-4217-y

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Effect of the 5-HT_7Ar gene on the reproductive performance of parthenogenetic Artemia

Biology | Updated：2025-10-16

- Effect of the 5-HT_7Ar gene on the reproductive performance of parthenogenetic Artemia
- Journal of Oceanology and Limnology Vol. 43, Issue 5, Pages: 1587-1595(2025)
- Affiliations：
  
  1.Asian Regional Artemia Reference Center, Key Laboratory of Marine Resource Chemistry and Food Technology (TUST), Tianjin University of Science and Technology, Tianjin 300450, China
  2.Institute of Aquatic Sciences, Xizang Autonomous Region Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850032, China
- Author bio：
  
  suily@tust.edu.cn
  hanxk@tust.edu.cn
- Funds：
- DOI：10.1007/s00343-025-4217-y
  CLC：
- Received：14 August 2024，
  
  Accepted：25 October 2024，
  
  Online First：27 November 2024，
  
  Published：01 September 2025
- Accepted：
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ZHANG Rui,OUYANG Xuemei,LI Ke,et al.Effect of the 5-HT_7Ar gene on the reproductive performance of parthenogenetic Artemia[J].Journal of Oceanology and Limnology,2025,43(05):1587-1595.
DOI：

ZHANG Rui,OUYANG Xuemei,LI Ke,et al.Effect of the 5-HT_7Ar gene on the reproductive performance of parthenogenetic Artemia[J].Journal of Oceanology and Limnology,2025,43(05):1587-1595. DOI： 10.1007/s00343-025-4217-y.

摘要

Abstract

5-hydroxytryptamine (5-HT) is a biological monoamine neurotransmitter in the central nervous system and gonads of crustaceans to induce gonadal maturation. To better understand the effects of 5-HT and its receptors on reproductive development in

Artemia

a 5-HT type 7 receptor gene (

7Ar

) was identified in parthenogenetic

Artemia

and characterized. Sequence analysis revealed that the open reading frame of

7Ar

encodes a 414-aa protein.

7Ar

showed higher expression in both brain and ovary at early embryo stage

7Ar

could be detected during ovarian development and the highest expression was observed at early embryo stage. Silencing of the

7Ar

Artemia

at early embryo stage decreased significantly the expression level of

7Ar

gene and protein at late oocyte

early embryo

and late embryo stage. Moreover

silencing of the

7Ar

resulted in a decreased fecundity

which corresponds to abnormal oocytes during the embryo development.

Artemia

tended to produce nauplii after the silencing of

7Ar

indicating that

7Ar

may also involve in the determination of its reproduction mode. The findings of this study provide an insight into the regulation of reproductive development in

Artemia

and the function of

7Ar

关键词

Keywords

references

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Related Institution

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⁰

Effect of the 5-HT7Ar gene on the reproductive performance of parthenogenetic Artemia

DOI：10.1007/s00343-025-4217-y

摘要

Abstract

关键词

Keywords

references

Effect of the 5-HT_7Ar gene on the reproductive performance of parthenogenetic Artemia