Indole enhanced the bactericidal efficacy of tetracycline against pathogen <italic style="font-style: italic">Vibrio splendidus</italic>

Jinxia ZHANG; Zhixin MU; Qiling ZHOU; Weiwei ZHANG

doi:10.1007/s00343-025-4202-5

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Indole enhanced the bactericidal efficacy of tetracycline against pathogen Vibrio splendidus

Biology | Updated：2025-10-16

- Indole enhanced the bactericidal efficacy of tetracycline against pathogen Vibrio splendidus
- Journal of Oceanology and Limnology Vol. 43, Issue 5, Pages: 1623-1635(2025)
- Affiliations：
  
  School of Marine Sciences, Ningbo University, Ningbo 315832, China
- Author bio：
  
  zhangweiwei1@nbu.edu.cn
- Funds：
- DOI：10.1007/s00343-025-4202-5
  CLC：
- Received：24 July 2024，
  
  Accepted：16 October 2024，
  
  Published：01 September 2025
- Accepted：
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ZHANG Jinxia,MU Zhixin,ZHOU Qiling,et al.Indole enhanced the bactericidal efficacy of tetracycline against pathogen Vibrio splendidus[J].Journal of Oceanology and Limnology,2025,43(05):1623-1635.
DOI：

ZHANG Jinxia,MU Zhixin,ZHOU Qiling,et al.Indole enhanced the bactericidal efficacy of tetracycline against pathogen Vibrio splendidus[J].Journal of Oceanology and Limnology,2025,43(05):1623-1635. DOI： 10.1007/s00343-025-4202-5.

摘要

Abstract

Recently

more and more bacteria have been reported to become tolerant to antibiotics. In this study

one

tnaA

gene involved in indole production

and the effect of exogenous indole on the formation of persister cells specific to tetracycline in

Vibrio

splendidus

were characterized. The

tnaA

gene was first cloned and conditionally expressed in

Escherichia

coli

Rosetta (DE3). To investigate the regulatory effect of TnaA

the

tnaA

deletion strain AJ01/∆

tnaA

was constructed by in-frame deletion. The undetected extracellular indole in the AJ01/∆

tnaA

indicated that TnaA was the solo enzyme to produce indole in

splendidus

. The drop plate method showed that AJ01/∆

tnaA

was more tolerant to the higher concentration of tetracycline than that of AJ01

being 340-fold higher in the proportion of survived cells when cell density OD

600

≈0.65. Moreover

the synergistic effects of indole and tetracycline on killing of

splendidus

were determined. Results show that addition of 2-mmol/L indole increased the susceptibility of both AJ01 and AJ01/∆

tnaA

to 10×minimum inhibitory concentration tetracycline. To explore the genes and pathways regulated by TnaA

the transcriptomic analysis between AJ01 and AJ01/∆

tnaA

was performed. Result shows that TCA cycle

arginine biosynthesis

quorum sensing and microbial metabolism in diverse environments were downregulated

while the ribosome pathways

the protein metabolic process

peptide biosynthetic and metabolic process were upregulated in the AJ01/∆

tnaA

. This study shows that indole could enhance the bactericidal effect of tetracycline on

splendidus

by decreased ribosome level probably but increased ATP level.

关键词

Keywords

references

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Indole enhanced the bactericidal efficacy of tetracycline against pathogen Vibrio splendidus

DOI：10.1007/s00343-025-4202-5

摘要

Abstract

关键词

Keywords

references