<italic style="font-style: italic">Halomonas</italic> sp. NEC-1, an ectoine-rich and wide pH tolerant halophilic bacterium isolated from a salt lake of Xizang, China

Yingchao MA; Zhongshi SUN; Tashilhamo; Yukang LI; Hanying ZOU; Yuanliang ZHAO; Feiyang LIN; Xuekai HAN; Liying SUI; Chi ZHANG

doi:10.1007/s00343-024-4168-8

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Halomonas sp. NEC-1, an ectoine-rich and wide pH tolerant halophilic bacterium isolated from a salt lake of Xizang, China

Biology | Updated：2025-10-16

- Halomonas sp. NEC-1, an ectoine-rich and wide pH tolerant halophilic bacterium isolated from a salt lake of Xizang, China
- Journal of Oceanology and Limnology Vol. 43, Issue 5, Pages: 1636-1646(2025)
- Affiliations：
  
  1.Asian Regional Artemia Reference Center, College of Marine and Environmental Sciences, Tianjin University of Science and Technology, Tianjin 300457, China
  2.Institute of Fisheries Science, Xizang Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850000, China
- Author bio：
  
  zc0891@163.com
- Funds：
- DOI：10.1007/s00343-024-4168-8
  CLC：
- Received：21 June 2024，
  
  Published：01 September 2025
- Accepted：
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MA Yingchao,SUN Zhongshi,Tashilhamo ,et al.Halomonas sp. NEC-1, an ectoine-rich and wide pH tolerant halophilic bacterium isolated from a salt lake of Xizang, China[J].Journal of Oceanology and Limnology,2025,43(05):1636-1646.
DOI：

MA Yingchao,SUN Zhongshi,Tashilhamo ,et al.Halomonas sp. NEC-1, an ectoine-rich and wide pH tolerant halophilic bacterium isolated from a salt lake of Xizang, China[J].Journal of Oceanology and Limnology,2025,43(05):1636-1646. DOI： 10.1007/s00343-024-4168-8.

摘要

Abstract

A halophilic bacterium

named

Halomonas

sp. NEC-1 was isolated from the Nyer Co Salt Lake on the Xizang Plateau

SW China. The strain exhibited a broad pH tolerance range of 5–11

distinguishing it from

Halomonas

alkaliphila

despite a 99% genetic similarity. Optimal growth conditions

determined through orthogonal experiments

were found to be 37 °C

100-g/L salinity

and an initial pH of 6

resulting in a maximum OD

600

of 7.98±0.06.

Halomonas

sp. NEC-1 produced 545.43±25.10 mg/L of ectoine under optimal conditions of 75-g/L salinity

40-g/L sodium glutamate

and an initial pH of 6. This production increased to 1 388

.81±3.69 mg/L after five rounds of hypo-osmotic shocks. During the shocks

ectoine productivity remained stable at approximately 16.29±0.04 to 17.28±0.48 mg/(L·h)

representing a 43.40%–52.11% increase compared to the rate without any shock (11.36±1.05 mg/(L·h)). Additionally

the expression of the

ectABC

gene cluster

related to ectoine synthesis

significantly increased following the shocks

enhancing ectoine production. The ectoine extract demonstrated notable protective effects on

Escherichia

coli

and plasmid DNA. After 10 min of exposure at 60 ℃

the colony count of

coli

treated with ectoine extract increased by 342% compared to treatment with distilled water. Furthermore

the ectoine extract protected plasmid DNA from 2

2′-Azobis (2-methylpropionamidine) dihydrochloride-induced damage. This study highlights

Halomonas

sp. NEC-1 is a promising strain for ectoine production and underscores the potential of microbial resources in salt lakes from Xizang region.

关键词

Keywords

references

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⁰

Halomonas sp. NEC-1, an ectoine-rich and wide pH tolerant halophilic bacterium isolated from a salt lake of Xizang, China

DOI：10.1007/s00343-024-4168-8

摘要

Abstract

关键词

Keywords

references