Characterization and function analysis of a glucose-regulated protein 78 gene in <italic style="font-style: italic">Neocaridina</italic> <italic style="font-style: italic">denticulata</italic> <italic style="font-style: italic">sinensis</italic>

Dandan FENG; Yujie LIU; Cong WANG; Xiao LI; Huimin LI; Yuying SUN; Jiquan ZHANG

doi:10.1007/s00343-024-4137-2

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Characterization and function analysis of a glucose-regulated protein 78 gene in Neocaridina denticulata sinensis

Biology | Updated：2025-08-07

- Characterization and function analysis of a glucose-regulated protein 78 gene in Neocaridina denticulata sinensis
- Journal of Oceanology and Limnology Vol. 43, Issue 4, Pages: 1267-1281(2025)
- Affiliations：
  
  1.School of Life Sciences/Hebei Basic Science Center for Biotic Interaction, Hebei University, Baoding 071002, China
  2.Institute of Life Science and Green Development, Hebei University, Baoding 071002, China
- Author bio：
  
  zhangjiquan@hbu.edu.cn
- Funds：
- DOI：10.1007/s00343-024-4137-2
  CLC：
- Received：14 May 2024，
  
  Published：01 July 2025
- Accepted：
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FENG Dandan,LIU Yujie,WANG Cong,et al.Characterization and function analysis of a glucose-regulated protein 78 gene in Neocaridina denticulata sinensis[J].Journal of Oceanology and Limnology,2025,43(04):1267-1281.
DOI：

FENG Dandan,LIU Yujie,WANG Cong,et al.Characterization and function analysis of a glucose-regulated protein 78 gene in Neocaridina denticulata sinensis[J].Journal of Oceanology and Limnology,2025,43(04):1267-1281. DOI： 10.1007/s00343-024-4137-2.

摘要

Abstract

Endoplasmic reticulum (ER) homeostasis is vital to cell physiological functions. Glucose-regulated protein 78 (GRP78) is a molecular chaperone considered a sensor of ER stress and participates in maintaining ER homeostasis. However

very few studies have provided detailed descriptions of crustacean GRP78 genes. The complete coding sequence of the GRP78 of

Neocaridina

denticulata

sinensis

was obtained and termed

NdGRP78

in this study. Sequence analysis showed that the open reading frame of

NdGRP78

encodes a protein of 656 amino acid residues

and the encoded protein includes three heat shock proteins 70 (HSP70) family conservational motifs

one ATP/GTP binding site A

and one ER homolog region. Multiple sequence alignments demonstrated that the sequences of these key protein functional domains were highly conserved. Phylogenetic analysis revealed that the NdGRP78 protein belongs to a member of the HSP70 family. The

NdGRP78

mRNA was detected in all tested tissues by real-time fluorescence quantitative PCR (qPCR)

suggesting that the

NdGRP78

mRNA was the constituent expression in

denticulata

sinensis

. The in-situ hybridization result showed that the expression of

NdGRP78

mRNA occurred in all types of hepatopancreas cells. Furthermore

the

NdGRP78

expression was significantly increased when shrimp were transferred in

Vibrio

parahaemolyticus

EGFP

infected conditions

low or high temperature

and hypoxia. The above results indicate that

NdGRP78

expressions were associated with stress responses. This study provided a theoretical support to the good understanding of the role of

NdGRP78

in response to different types of environmental stress tolerance.

关键词

Keywords

references

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⁰

Characterization and function analysis of a glucose-regulated protein 78 gene in Neocaridina denticulata sinensis

DOI：10.1007/s00343-024-4137-2

摘要

Abstract

关键词

Keywords

references