RNA interference with <italic style="font-style: italic">foxl2</italic> leads to upregulation of <italic style="font-style: italic">cyp19b</italic> and delayed development of female carp <italic style="font-style: italic">Cyprinus</italic> <italic style="font-style: italic">carpio</italic> var. <italic style="font-style: italic">koi</italic> gonads

Xiao MEI; Xinglei WANG; Qiang ZHOU; Bo LIU; Yishun WANG; Liuchang KONG; Shibo JIANG; Feng WANG

doi:10.1007/s00343-024-4113-x

Your Location：

Home >

Browse articles >

RNA interference with foxl2 leads to upregulation of cyp19b and delayed development of female carp Cyprinus carpio var. koi gonads

Biology | Updated：2025-05-27

- RNA interference with foxl2 leads to upregulation of cyp19b and delayed development of female carp Cyprinus carpio var. koi gonads
- Journal of Oceanology and Limnology Vol. 43, Issue 3, Pages: 954-966(2025)
- Affiliations：
  
  1.College of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China
  2.Qingdao Marine Science and Technology Museum, Qingdao 266003, China
  3.Shandong Tangwangli Agricultural Development Co., Ltd., Jinan 250106, China
- Author bio：
  
  randy966@163.com
- Funds：
- DOI：10.1007/s00343-024-4113-x
  CLC：
- Received：20 April 2024，
  
  Online First：29 July 2024，
  
  Published：01 May 2025
- Accepted：
Scan QR Code
MEI Xiao,WANG Xinglei,ZHOU Qiang,et al.RNA interference with foxl2 leads to upregulation of cyp19b and delayed development of female carp Cyprinus carpio var. koi gonads[J].Journal of Oceanology and Limnology,2025,43(03):954-966.
DOI：

MEI Xiao,WANG Xinglei,ZHOU Qiang,et al.RNA interference with foxl2 leads to upregulation of cyp19b and delayed development of female carp Cyprinus carpio var. koi gonads[J].Journal of Oceanology and Limnology,2025,43(03):954-966. DOI： 10.1007/s00343-024-4113-x.

摘要

Abstract

FOXL2 and CYP19B are crucial transcription factors in vertebrates and invertebrates that play pivotal roles in sex differentiation and gonadal development. The potential roles of the

foxl2

and

cyp19b

genes in sex determination and gonadal development in

Cyprinus

carpio

var.

koi

were explored using a non-invasive RNA interference (RNAi) method

histopathological observation and qPCR. Results demonstrate that

foxl2

exhibited a sexually dimorphic expression pattern in gonads

with a notable expression in ovaries;

cyp19b

was expressed in all peripheral tissues

with a particularly prominent expression in brain and gonads. The knockdown of

foxl2

by RNAi resulted in delay in the development of the female gonads. Conversely

no notable alterations were discerned in the gonads of

carpio

var.

koi

following the knockdown of

cyp19b

. The upregulation of

sox9a

amh

and

cyp19b

following

foxl

2 knockdown indicates that

foxl2

may play a pivotal role in gonadal development. Nevertheless

further investigation is required to ascertain the potential role of

cyp19b

. This study elucidated the role of

foxl2

and enhanced the understanding of the mechanisms of sex determination and gonadal development in

carpio

var.

koi

关键词

Keywords

references

Abdel-Tawwab M , Hagras A E , Elbaghdady H A M et al . 2014 . Dissolved oxygen level and stocking density effects on growth, feed utilization, physiology, and innate immunity of Nile tilapia, Oreochromis niloticus . Journal of Applied Aquaculture , 26 ( 4 ): 340 - 355 , https://doi.org/10.1080/10454438.2014.959830 https://doi.org/10.1080/10454438.2014.959830 .

Bao L S , Tian C X , Liu S K et al . 2019 . The Y chromosome sequence of the channel catfish suggests novel sex determination mechanisms in teleost fish . BMC Biology , 17 ( 1 ): 6 , https://doi.org/10.1186/s12915-019-0627-7 https://doi.org/10.1186/s12915-019-0627-7 .

Baron D , Cocquet J , Xia X H et al . 2004 . An evolutionary and functional analysis of FoxL2 in rainbow trout gonad differentiation . Journal of Molecular Endocrinology , 33 ( 3 ): 705 - 715 , https://doi.org/10.1677/jme.1.01566 https://doi.org/10.1677/jme.1.01566 .

Bertho S , Herpin A , Schartl M et al . 2021 . Lessons from an unusual vertebrate sex-determining gene . Philosophical Transactions of the Royal Society B: Biological Sciences , 376 ( 1832 ): 20200092 , https://doi.org/10.1098/rstb.2020.0092 https://doi.org/10.1098/rstb.2020.0092 .

Bertho S , Pasquier J , Pan Q et al . 2016 . Foxl2 and its relatives are evolutionary conserved players in gonadal sex differentiation . Sexual Development , 10 ( 3 ): 111 - 129 , https://doi.org/10.1159/000447611 https://doi.org/10.1159/000447611 .

Bharathi J K , Anandan R , Benjamin L K et al . 2023 . Recent trends and advances of RNA interference (RNAi) to improve agricultural crops and enhance their resilience to biotic and abiotic stresses . Plant Physiology and Biochemistry , 194 : 600 - 618 , https://doi.org/10.1016/j.plaphy.2022.11.035 https://doi.org/10.1016/j.plaphy.2022.11.035 .

Bhattarai C , Poudel P P , Ghosh A et al . 2022 . Comparative role of SOX10 gene in the gliogenesis of central, peripheral, and enteric nervous systems . Differentiation , 128 : 13 - 25 , https://doi.org/10.1016/j.diff.2022.09.001 https://doi.org/10.1016/j.diff.2022.09.001 .

Brinton R D . 2009 . Estrogen-induced plasticity from cells to circuits: predictions for cognitive function . Trends in Pharmacological Sciences , 30 ( 4 ): 212 - 222 , https://doi.org/10.1016/j.tips.2008.12.006 https://doi.org/10.1016/j.tips.2008.12.006 .

Cano-Nicolau J , Garoche C , Hinfray N et al . 2016 . Several synthetic progestins disrupt the glial cell specific-brain aromatase expression in developing zebra fish . Toxicology and Applied Pharmacology , 305 : 12 - 21 , https://doi.org/10.1016/j.taap.2016.05.019 https://doi.org/10.1016/j.taap.2016.05.019 .

Dalaisón-Fuentes L I , Pascual A , Crespo M et al . 2023 . Knockdown of double - stranded RNases ( dsRNases ) enhances oral RNA interference (RNAi) in the corn leafhopper, Dalbulus maidis . Pesticide Biochemistry and Physiology , 196 : 105618 , https://doi.‍org/10.‍1016/j.pestbp.2023.105618 https://doi.‍org/10.‍1016/j.pestbp.2023.105618 . https://do 10.1016/j.pestbp.2023.105618 http://dx.doi.org/10.1016/j.pestbp.2023.105618

Delalande C , Goupil A S , Lareyre J J et al . 2015 . Differential expression patterns of three aromatase genes and of four estrogen receptors genes in the testes of trout ( Oncorhynchus mykiss ) . Molecular Reproduction and Development , 82 ( 9 ): 694 - 708 , https://doi.org/10.1002/mrd.22509 https://doi.org/10.1002/mrd.22509 .

Fan Z F , Zou Y X , Liang D D et al . 2019 . Roles of forkhead box protein L2 ( foxl2 ) during gonad differentiation and maintenance in a fish, the olive flounder ( Paralichthys olivaceus ) . Reproduction, Fertility and Development , 31 ( 11 ): 1742 - 1752 , https://doi.org/10.1071/RD18233 https://doi.org/10.1071/RD18233 .

Fent K , Siegenthaler P F , Schmid A A . 2018 . Transcriptional effects of androstenedione and 17α-hydroxyprogesterone in zebrafish embryos . Aquatic Toxicology , 202 : 1 - 5 , https://doi.org/10.1016/j.aquatox.2018.06.012 https://doi.org/10.1016/j.aquatox.2018.06.012 .

Fire A , Xu S Q , Montgomery M K et al . 1998 . Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans . Nature , 391 ( 6669 ): 806 - 811 , https://doi.org/10.1038/35888 https://doi.org/10.1038/35888 .

Fischer A H , Jacobson K A , Rose J et al . 2008 . Hematoxylin and eosin staining of tissue and cell sections . Cold Spring Harbor Protocols , 2008 ( 5 ): pdb.prot 4986 , https://doi.org/10.1101/pdb.prot4986 https://doi.org/10.1101/pdb.prot4986 .

Germain N D , Chung W K , Sarmiere P D . 2023 . RNA interference (RNAi)-based therapeutics for treatment of rare neurologic diseases . Molecular Aspects of Medicine , 91 : 101148 , https://doi.org/10.1016/j.mam.2022.101148 https://doi.org/10.1016/j.mam.2022.101148 .

González A , Piferrer F . 2003 . Aromatase activity in the European sea bass ( Dicentrarchus labrax L.) brain. Distribution and changes in relation to age, sex, and the annual reproductive cycle . General and Comparative Endocrinology , 132 ( 2 ): 223 - 230 , https://doi.org/10.1016/S0016-6480(03)00086-8 https://doi.org/10.1016/S0016-6480(03)00086-8 .

Guiguen Y , Fostier A , Piferrer F et al . 2010 . Ovarian aromatase and estrogens: a pivotal role for gonadal sex differentiation and sex change in fish . General and Comparative Endocrinology , 165 ( 3 ): 352 - 366 , https://doi.org/10.1016/j.ygcen.2009.03.002 https://doi.org/10.1016/j.ygcen.2009.03.002 .

Hu Q , Guo W , Gao Y et al . 2014 . Molecular cloning and analysis of gonadal expression of Foxl2 in the rice-field eel Monopterus albus . Scientific Reports , 4 ( 1 ): 6884 , https://doi.org/10.1038/srep06884 https://doi.org/10.1038/srep06884 .

Hu Y N , Fu Y , Jin S B et al . 2021 . Comparative transcriptome analysis of lethality in response to RNA interference of the oriental river prawn ( Macrobrachium nipponense ) . Comparative Biochemistry and Physiology Part D: Genomics and Proteomics , 38 : 100802 , https://doi.org/10.1016/j.cbd.2021.100802 https://doi.org/10.1016/j.cbd.2021.100802 .

Huang S S , Ye L P , Chen H L . 2017 . Sex determination and maintenance: the role of DMRT1 and FOXL2 . Asian Journal of Andrology , 19 ( 6 ): 619 - 624 , https://doi.‍org/10.4103/1008-682x.194420 https://doi.‍org/10.4103/1008-682x.194420 . https://do 10.4103/1008-682x.194420 http://dx.doi.org/10.4103/1008-682x.194420

Huvenne H , Smagghe G . 2010 . Mechanisms of dsRNA uptake in insects and potential of RNAi for pest control: a review . Journal of Insect Physiology , 56 ( 3 ): 227 - 235 , https://doi.org/10.1016/j.jinsphys.2009.10.004 https://doi.org/10.1016/j.jinsphys.2009.10.004 .

Jiang W B , Yang Y H , Zhao D M et al . 2011 . Effects of sexual steroids on the expression of foxl2 in Gobiocypris rarus . Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology , 160 ( 4 ): 187 - 193 , https://doi.org/10.1016/j.cbpb.2011.08.005 https://doi.org/10.1016/j.cbpb.2011.08.005 .

Jin L , Sun W , Bao H S et al . 2022 . The forkhead factor Foxl2 participates in the ovarian differentiation of Chinese soft-shelled turtle Pelodiscus sinensis . Developmental Biology , 492 : 101 - 110 , https://doi.org/10.1016/j.ydbio.2022.10.001 https://doi.org/10.1016/j.ydbio.2022.10.001 .

Josso N , di Clemente N , Gouédard L . 2001 . Anti-Müllerian hormone and its receptors . Molecular and Cellular Endocrinology , 179 ( 1-2 ): 25 - 32 , https://doi.‍org/10.1016/s0303-7207(01)00467-1 https://doi.‍org/10.1016/s0303-7207(01)00467-1 . https://do 10.1016/s0303-7207(01)00467-1 http://dx.doi.org/10.1016/s0303-7207(01)00467-1

Kadotani N , Nakayashiki H , Tosa Y et al . 2004 . One of the two dicer-like proteins in the filamentous fungi Magnaporthe oryzae genome is responsible for hairpin RNA-triggered RNA silencing and related small interfering RNA accumulation . Journal of Biological Chemistry , 279 ( 43 ): 44467 - 44474 , https://doi.org/10.1074/jbc.M408259200 https://doi.org/10.1074/jbc.M408259200 .

Kallivretaki E , Eggen R I L , Neuhauss S C F et al . 2007 . The zebrafish, brain-specific, aromatase cyp19a2 is neither expressed nor distributed in a sexually dimorphic manner during sexual differentiation . Developmental Dynamics , 236 ( 11 ): 3155 - 3166 , https://doi.org/10.1002/dvdy.21344 https://doi.org/10.1002/dvdy.21344 .

Le Page Y , Diotel N , Vaillant C et al . 2010 . Aromatase, brain sexualization and plasticity: the fish paradigm . European Journal of Neuroscience , 32 ( 12 ): 2105 - 2115 , https://doi.org/10.1111/j.1460-9568.2010.07519.x https://doi.org/10.1111/j.1460-9568.2010.07519.x .

Lee Y S , Nakahara K , Pham J W et al . 2004 . Distinct roles for Drosophila dicer-1 and dicer-2 in the siRNA/miRNA silencing pathways . Cell , 117 ( 1 ): 69 - 81 , https://doi.org/10.1016/s0092-8674(04)00261-2 https://doi.org/10.1016/s0092-8674(04)00261-2 .

Liu X Y , Zhang X B , Li M H et al . 2017 . Genome-wide identification, evolution of chromobox family genes and their expression in Nile tilapia . Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology , 203 : 25 - 34 , https://doi.‍org/10.1016/j.cbpb.2016.09.001 https://doi.‍org/10.1016/j.cbpb.2016.09.001 . https://do 10.1016/j.cbpb.2016.09.001 http://dx.doi.org/10.1016/j.cbpb.2016.09.001

Livak K J , Schmittgen T D . 2001 . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method . Methods , 25 ( 4 ): 402 - 408 , https://doi.org/10.1006/meth.2001.1262 https://doi.org/10.1006/meth.2001.1262 .

Major A T , Ayers K L , Chue J et al . 2019 . FOXL2 antagonises the male developmental pathway in embryonic chicken gonads . Journal of Endocrinology , 243 ( 3 ): 211 - 228 , https://doi.‍org/10.1530/JOE-19-0277 https://doi.‍org/10.1530/JOE-19-0277 . https://do 10.1530/joe-19-0277 http://dx.doi.org/10.1530/joe-19-0277

Matsuoka M P , van Nes S , Andersen Ø et al . 2006 . Real-time PCR analysis of ovary- and brain-type aromatase gene expression during Atlantic halibut ( Hippoglossus hippoglossus ) development . Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology , 144 ( 1 ): 128 - 135 , https://doi.‍org/10.1016/j.‍cbpb.2006.02.008 https://doi.‍org/10.1016/j.‍cbpb.2006.02.008 . https://do 10.1016/j.cbpb.2006.02.008 http://dx.doi.org/10.1016/j.cbpb.2006.02.008

Mello C C , Conte D . 2004 . Revealing the world of RNA interference . Nature , 431 ( 7006 ): 338 - 342 , https://doi.org/10.1038/nature02872 https://doi.org/10.1038/nature02872 .

Nagahama Y , Chakraborty T , Paul-Prasanth B et al . 2021 . Sex determination, gonadal sex differentiation, and plasticity in vertebrate species . Physiological Reviews , 101 ( 3 ): 1237 - 1308 , https://doi.org/10.1152/physrev.00044.2019 https://doi.org/10.1152/physrev.00044.2019 .

Nagakura R , Yamamoto M , Jeong J et al . 2020 . Switching of Sox9 expression during musculoskeletal system development . Scientific Reports , 10 ( 1 ): 8425 , https://doi.org/10.1038/s41598-020-65339-9 https://doi.org/10.1038/s41598-020-65339-9 .

Nakamoto M , Matsuda M , Wang D S et al . 2006 . Molecular cloning and analysis of gonadal expression of Foxl2 in the medaka, Oryzias latipes . Biochemical and Biophysical Research Communications , 344 ( 1 ): 353 - 361 , https://doi.org/10.1016/j.bbrc.2006.03.137 https://doi.org/10.1016/j.bbrc.2006.03.137 .

Nicol B , Grimm S A , Gruzdev A et al . 2018 . Genome-wide identification of FOXL2 binding and characterization of FOXL2 feminizing action in the fetal gonads . Human Molecular Genetics , 27 ( 24 ): 4273 - 4287 , https://doi.org/10.1093/hmg/ddy312 https://doi.org/10.1093/hmg/ddy312 .

Ning J H , Cao W A , Lu X et al . 2021 . Identification and functional analysis of a sex-biased transcriptional factor Foxl2 in the bay scallop Argopecten irradians irradians . Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology , 256 : 110638 , https://doi.org/10.1016/j.cbpb.2021.110638 https://doi.org/10.1016/j.cbpb.2021.110638 .

Patil J G , Gunasekera R M . 2008 . Tissue and sexually dimorphic expression of ovarian and brain aromatase mRNA in the Japanese medaka ( Oryzias latipes ): implications for their preferential roles in ovarian and neural differentiation and development . General and Comparative Endocrinology , 158 ( 1 ): 131 - 137 , https://doi.org/10.1016/j.ygcen.2008.05.016 https://doi.org/10.1016/j.ygcen.2008.05.016 .

Ramallo M R , Honji R M , Birba A et al . 2017 . A game of two? Gene expression analysis of brain ( cyp19a1b ) and gonadal ( cyp19a1a ) aromatase in females of a Neotropical cichlid fish through the parental care period and removal of the offspring . General and Comparative Endocrinology , 252 : 119 - 129 , https://doi.org/10.1016/j.ygcen.2017.08.009 https://doi.org/10.1016/j.ygcen.2017.08.009 .

Ramee S W , Lipscomb T N , DiMaggio M A . 2020 . Evaluation of the effect of larval stocking density, salinity, and temperature on stress response and sex differentiation in the Dwarf Gourami and Rosy Barb . Aquaculture Reports , 16 : 100287 , https://doi.‍org/10.1016/j.aqrep.2020.100287 https://doi.‍org/10.1016/j.aqrep.2020.100287 . https://do 10.1016/j.aqrep.2020.100287 http://dx.doi.org/10.1016/j.aqrep.2020.100287

Sacchi S , Marinaro F , Xella S et al . 2017 . The anti-Müllerian hormone (AMH) induces forkhead box L2 (FOXL2) expression in primary culture of human granulosa cells in vitro . Journal of Assisted Reproduction and Genetics , 34 ( 9 ): 1131 - 1136 , https://doi.org/10.1007/s10815-017-0980-9 https://doi.org/10.1007/s10815-017-0980-9 .

Sekido R , Lovell-Badge R . 2008 . Sex determination involves synergistic action of SRY and SF1 on a specific Sox9 enhancer . Nature , 453 ( 7197 ): 930 - 934 , https://doi.org/10.1038/nature06944 https://doi.org/10.1038/nature06944 .

Shen Z G , Eissa N , Yao H et al . 2018 . Effects of temperature on the expression of two ovarian differentiation-related genes foxl2 and cyp19a1a . Frontiers in Physiology , 9 : 1208 , https://doi.org/10.3389/fphys.2018.01208 https://doi.org/10.3389/fphys.2018.01208 .

Silva de Assis H C , Navarro-Martín L , Fernandes L S P et al . 2018 . Cloning, partial sequencing and expression analysis of the neural form of P450 aromatase ( cyp19a1b ) in the South America catfish Rhamdia quelen . Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology , 221 - 222 : 11 - 17 , https://doi.‍org/10.1016/j.cbpb.2018.04.001 https://doi.‍org/10.1016/j.cbpb.2018.04.001 . https://do 10.1016/j.cbpb.2018.04.001 http://dx.doi.org/10.1016/j.cbpb.2018.04.001

Simpson E R , Merrill J C , Hollub A J et al . 1989 . Regulation of estrogen biosynthesis by human adipose cells . Endocrine Reviews , 10 ( 2 ): 136 - 148 , https://doi.org/10.1210/edrv-10-2-136 https://doi.org/10.1210/edrv-10-2-136 .

Sridevi P , Senthilkumaran B . 2011 . Cloning and differential expression of FOXL2 during ovarian development and recrudescence of the catfish, Clarias gariepinus . General and Comparative Endocrinology , 174 ( 3 ): 259 - 268 , https://doi.org/10.1016/j.ygcen.2011.08.015 https://doi.org/10.1016/j.ygcen.2011.08.015 .

Tang B , Hu W , Hao J et al . 2010 . Developmental expression of steroidogenic factor-1, cyp19a1a and cyp19a1b from common carp ( Cyprinus carpio ) . General and Comparative Endocrinology , 167 ( 3 ): 408 - 416 , https://doi.‍org/10.‍1016/j.ygcen.2010.03.017 https://doi.‍org/10.‍1016/j.ygcen.2010.03.017 . https://do 10.1016/j.ygcen.2010.03.017 http://dx.doi.org/10.1016/j.ygcen.2010.03.017

Tessema A , Getahun A , Mengistou S et al . 2020 . Reproductive biology of common carp ( Cyprinus carpio Linnaeus, 1758) in Lake Hayq, Ethiopia . Fisheries and Aquatic Sciences , 23 ( 1 ): 16 , https://doi.org/10.1186/s41240-020-00162-x https://doi.org/10.1186/s41240-020-00162-x .

Uhlenhaut N H , Jakob S , Anlag K et al . 2009 . Somatic sex reprogramming of adult ovaries to testes by FOXL2 ablation . Cell , 139 ( 6 ): 1130 - 1142 , https://doi.‍org/10.1016/j.cell.2009.11.021 https://doi.‍org/10.1016/j.cell.2009.11.021 . https://do 10.1016/j.cell.2009.11.021 http://dx.doi.org/10.1016/j.cell.2009.11.021

von Schalburg K R , Yasuike M , Davidson W S et al . 2010 . Regulation, expression and characterization of aromatase ( cyp19b1 ) transcripts in ovary and testis of rainbow trout ( Oncorhynchus mykiss ) . Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology , 155 ( 2 ): 118 - 125 , https://doi.‍org/10.1016/j.‍cbpb.2009.10.015 https://doi.‍org/10.1016/j.‍cbpb.2009.10.015 . https://do 10.1016/j.cbpb.2009.10.015 http://dx.doi.org/10.1016/j.cbpb.2009.10.015

Wang K , Zhao J N , Han Z J et al . 2022 . Comparative transcriptome and RNA interference reveal CYP6DC1 and CYP380C47 related to lambda-cyhalothrin resistance in Rhopalosiphum padi . Pesticide Biochemistry and Physiology , 183 : 105088 , https://doi.org/10.1016/j.pestbp.2022.105088 https://doi.org/10.1016/j.pestbp.2022.105088 .

Wang M , Chen L , Zhou Z X et al . 2023 . Comparative transcriptome analysis of early sexual differentiation in the male and female gonads of common carp ( Cyprinus carpio ) . Aquaculture , 563 : 738984 , https://doi.org/10.1016/j.aquaculture.2022.738984 https://doi.org/10.1016/j.aquaculture.2022.738984 .

Webster K A , Schach U , Ordaz A et al . 2017 . Dmrt1 is necessary for male sexual development in zebrafish . Developmental Biology , 422 ( 1 ): 33 - 46 , https://doi.‍org/10.1016/j.ydbio.2016.12.008 https://doi.‍org/10.1016/j.ydbio.2016.12.008 . https://do 10.1016/j.ydbio.2016.12.008 http://dx.doi.org/10.1016/j.ydbio.2016.12.008

Xing L , Goswami M , Trudeau V L . 2014 . Radial glial cell: critical functions and new perspective as a steroid synthetic cell . General and Comparative Endocrinology , 203 : 181 - 185 , https://doi.org/10.1016/j.ygcen.2014.03.010 https://doi.org/10.1016/j.ygcen.2014.03.010 .

Yamaguchi T , Yamaguchi S , Hirai T et al . 2007 . Follicle-stimulating hormone signaling and Foxl2 are involved in transcriptional regulation of aromatase gene during gonadal sex differentiation in Japanese flounder, Paralichthys olivaceus . Biochemical and Biophysical Research Communications , 359 ( 4 ): 935 - 940 , https://doi.org/10.1016/j.bbrc.2007.05.208 https://doi.org/10.1016/j.bbrc.2007.05.208 .

Yang Y J , Wang Y , Li Z et al . 2017 . Sequential, divergent, and cooperative requirements of Foxl2a and Foxl2b in ovary development and maintenance o f zebrafish . Genetics , 205 ( 4 ): 1551 - 1572 , https://doi.‍org/10.1534/genetics.116.199133 https://doi.‍org/10.1534/genetics.116.199133 . https://do 10.1534/genetics.116.199133 http://dx.doi.org/10.1534/genetics.116.199133

Zhang X B , Li M R , Ma H et al . 2017 . Mutation of foxl2 or cyp19a1a results in female to male sex reversal in XX Nile tilapia . Endocrinology , 158 ( 8 ): 2634 - 2647 , https://doi.org/10.1210/en.2017-00127 https://doi.org/10.1210/en.2017-00127 .

Zhou L Y , Li M H , Wang D S . 2021 . Role of sex steroids in fish sex determination and differentiation as revealed by gene editing . General and Comparative Endocrinology , 313 : 113893 , https://doi.org/10.1016/j.ygcen.2021.113893 https://doi.org/10.1016/j.ygcen.2021.113893 .

Zhu Y K , Chen H L , Zhang Q F et al . 2023 . Molecular cloning and expression patterns of a sex-biased transcriptional factor Foxl2 in the giant freshwater prawn ( Macrobrachium rosenbergii ) . Molecular Biology Reports , 50 ( 4 ): 3581 - 3591 , https://doi.‍org/10.1007/s11033-022-07526-7 https://doi.‍org/10.1007/s11033-022-07526-7 . https://do 10.1007/s11033-022-07526-7 http://dx.doi.org/10.1007/s11033-022-07526-7

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Sex determination and differentiation in Aurelia sp.1: the absence of temperature dependence

Identification of a novel C-type lectin from the shrimp Litopenaeus vannamei and its role in defense against pathogens infection

Effects of spectral composition, photoperiod and light intensity on the gonadal development of Atlantic salmon Salmo salar in recirculating aquaculture systems (RAS)

Performance Modelling of Geared Contra-RotatingPropfan Engine

Numerical Studies on Effects of V-Shaped Leading Edge on Incident Shock Wave Boundary Layer Interaction

Related Author

Feng WANG

Chunsheng LIU

Zhifeng GU

Mengxin XING

Yun SUN

Siqing CHEN

Zhaoting CHEN

Zhan Luo

Related Institution

State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University

Key Laboratory of Tropical Hydrobiogical Technology, Ocean College, Hainan University

Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences

Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences

Graduate University of Chinese Academy of Sciences

Address：Editorial Office of JOL, 88 Haijun Rd. Qingdao, 266000, China
Tel：+86-532-82898754 Email：jol@qdio.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰

RNA interference with foxl2 leads to upregulation of cyp19b and delayed development of female carp Cyprinus carpio var. koi gonads

DOI：10.1007/s00343-024-4113-x

摘要

Abstract

关键词

Keywords

references