Comparative transcriptome analysis revealed host defense responses in endosymbiotic gill of <italic style="font-style: italic">Bathymodiolus</italic> mussels inhabiting cold seeps and hydrothermal vents

Genmei LIN; Boxian WEN; Junrou HUANG; Xuanguang LIANG; Cuiling XU; Ye CHEN; Nengyou WU; Jianguo LU

doi:10.1007/s00343-024-4080-2

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Comparative transcriptome analysis revealed host defense responses in endosymbiotic gill of Bathymodiolus mussels inhabiting cold seeps and hydrothermal vents

Biology | Updated：2025-05-27

- Comparative transcriptome analysis revealed host defense responses in endosymbiotic gill of Bathymodiolus mussels inhabiting cold seeps and hydrothermal vents
- Journal of Oceanology and Limnology Vol. 43, Issue 3, Pages: 878-891(2025)
- Affiliations：
  
  1.School of Marine Sciences, Sun Yat-sen University, Zhuhai 519082, China
  2.Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519080, China
  3.Key Laboratory of Gas Hydrate, Ministry of Natural Resources, Qingdao Institute of Marine Geology, Qingdao 266071, China
  4.Laboratory for Marine Mineral Resources, Laoshan Laboratory, Qingdao 266237, China
- Author bio：
  
  wuny@ms.giec.ac.cn
  lujianguo@mail.sysu.edu.cn
- Funds：
- DOI：10.1007/s00343-024-4080-2
  CLC：
- Received：15 March 2024，
  
  Online First：02 July 2024，
  
  Published：01 May 2025
- Accepted：
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LIN Genmei,WEN Boxian,HUANG Junrou,et al.Comparative transcriptome analysis revealed host defense responses in endosymbiotic gill of ,Bathymodiolus mussels inhabiting cold seeps and hydrothermal vents[J].Journal of Oceanology and Limnology,2025,43(03):878-891.
DOI：

LIN Genmei,WEN Boxian,HUANG Junrou,et al.Comparative transcriptome analysis revealed host defense responses in endosymbiotic gill of ,Bathymodiolus mussels inhabiting cold seeps and hydrothermal vents[J].Journal of Oceanology and Limnology,2025,43(03):878-891. DOI： 10.1007/s00343-024-4080-2.

摘要

Abstract

Bathymodiolus

mussels distribute in both deep-sea cold seep and hydrothermal vent environments

whose endosymbiotic gill tissue is a prominent character for the adaptation of extreme habitats. However

few studies explored the adaptation mechanisms through comparative transcriptome sequencing and analysis of different tissues between seep mussels and vent mussels. We performed the comparative transcriptome sequencing and analysis for three tissue types (gill

mantle

and adductor muscle

) of

Bathymodiolus

mussels collected from a cold seeping site Station S11 and the 50-km away hydrothermal field Minami-Ensei Knoll in the Okinawa Trough. Results show that gene expression patterns had distinct tissue specificity. Compared with the non-endosymbiotic tissues (mantle and adductor muscle)

the significantly strengthened gene functions in endosymbiotic gill included microbial recognition (fibrinogen C domain-containing protein 1-B-like (

fibcd

)

fibrinogen-related protein 8 (

frp

)

peptidoglycan recognition proteins (

pgrp

)

and C-type lectin (

clec

))

cell apoptosis and immunity (interferon regulatory factor 1/2-like 1 (

irf

)

cathepsin D (

ctsd

)

caspase 2 (

casp2

))

and antioxidant capacity (copper/zinc superoxide dismutase (

czsod

)

glutathione peroxidase (

gpx

)

selenoprotein (

sel

))

in both seep and vent individuals. Consistent with metal accumulation

high expression levels of genes related to heavy metal detoxification (cytochrome P450 (

cyp

)

ferritin-like (

ftl

)

metallothionein (

)

glutathione S-transferase (

gst

)) were also observed in gill. Moreover

to adapt to high hydrostatic pressure in the deep sea

the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways associated with cellular community were significantly enriched in all three tissues

suggesting the regulation of cell structure and cell adhesion at transcriptional level. This study obtained gene expression profiles of deep-sea mussels subsisting at cold seep and hydrothermal vent sites

which could lay foundations for comprehensive investigations of molecular basis in adaptation of deep-sea mussels to the two extreme ecosystems.

关键词

Keywords

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Related Institution

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Comparative transcriptome analysis revealed host defense responses in endosymbiotic gill of Bathymodiolus mussels inhabiting cold seeps and hydrothermal vents

DOI：10.1007/s00343-024-4080-2

摘要

Abstract

关键词

Keywords

references