Size-dependent toxicity of microplastics induced gut cell apoptosis in brine shrimp <italic style="font-style: italic">Artemia</italic> <italic style="font-style: italic">parthenogenetica</italic> through <italic style="font-style: italic">p53</italic> signaling pathway

Junfang WANG; Zhichao YAN; Yulong LU; Wei XIE; Liying SUI

doi:10.1007/s00343-024-4066-0

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Size-dependent toxicity of microplastics induced gut cell apoptosis in brine shrimp Artemia parthenogenetica through p53 signaling pathway

Ecotoxicological implications for micro- and nano-plastics | Updated：2025-03-21

- Size-dependent toxicity of microplastics induced gut cell apoptosis in brine shrimp Artemia parthenogenetica through p53 signaling pathway
- Journal of Oceanology and Limnology Vol. 43, Issue 2, Pages: 614-623(2025)
- Affiliations：
  
  1.Asian Regional Artemia Reference Center, College of Marine and Environmental Sciences, Tianjin University of Science and Technology, Tianjin 300457, China
  2.Key Laboratory of Marine Resource Chemistry and Food Technology (TUST), Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, China
- Author bio：
  
  suily@tust.edu.cn
- Funds：
- DOI：10.1007/s00343-024-4066-0
  CLC：
- Received：04 March 2024，
  
  Online First：03 June 2024，
  
  Published：01 March 2025
- Accepted：
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WANG Junfang,YAN Zhichao,LU Yulong,et al.Size-dependent toxicity of microplastics induced gut cell apoptosis in brine shrimp Artemia parthenogenetica through p53 signaling pathway[J].Journal of Oceanology and Limnology,2025,43(02):614-623.
DOI：

WANG Junfang,YAN Zhichao,LU Yulong,et al.Size-dependent toxicity of microplastics induced gut cell apoptosis in brine shrimp Artemia parthenogenetica through p53 signaling pathway[J].Journal of Oceanology and Limnology,2025,43(02):614-623. DOI： 10.1007/s00343-024-4066-0.

摘要

Abstract

Microplastics (MPs) are one of the most concerning pollutants that affects the health and growth of aquatic organisms. We characterized the MPs dispersion in the milli-Q water and seawater

and evaluated the effects of MPs on the gut epithelial cells of brine shrimp using three sizes of polystyrene (PS) microbeads (0.05

0.5

and 5 μm

respectively). Results show that microbeads evenly dispersed in milli-Q water

but exhibited aggregation tendency in seawater associating with the particle size. Apart from a reduced survival rate

we observed the structure changes in the gut epithelium that the smaller size of PS microbeads resulted in an increased reactive oxygen species (ROS) and higher apoptosis-related genes expression. Moreover

exposure to all size of PS microbeads led to increased green fluorescence of J-monomer

indicating the declined mitochondrial membrane potential. Therefore

exposure to PS microbeads led to significantly size-dependent toxicity on brine shrimp. Especially

0.05-μm PS microbeads were more toxic

leading to severe oxidative stress and activation of the

p53

Bax

Bcl2

pathway

ultimately resulting in cellular apoptosis and gut damage. These findings are important to understand the mechanism of MPs toxicity and its potential ecological risks to marine aquatic animals.

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Keywords

references

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Size-dependent toxicity of microplastics induced gut cell apoptosis in brine shrimp Artemia parthenogenetica through p53 signaling pathway

DOI：10.1007/s00343-024-4066-0

摘要

Abstract

关键词

Keywords

references