A novel enzyme-assisted one-pot method for the extraction of fucoidan and alginate oligosaccharides from <italic style="font-style: italic">Lessonia</italic> <italic style="font-style: italic">trabeculata</italic> and their bioactivities

Changhui YAN; Mingxuan PAN; Lihua GENG; Quanbin ZHANG; Yadong HU; Jing WANG; Sujuan YE

doi:10.1007/s00343-024-4042-8

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A novel enzyme-assisted one-pot method for the extraction of fucoidan and alginate oligosaccharides from Lessonia trabeculata and their bioactivities

Biology | Updated：2024-12-24

- A novel enzyme-assisted one-pot method for the extraction of fucoidan and alginate oligosaccharides from Lessonia trabeculata and their bioactivities
- Journal of Oceanology and Limnology Vol. 42, Issue 6, Pages: 1998-2012(2024)
- Affiliations：
  
  1.College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao266061, China
  2.CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao266071, China
  3.Laboratory for Marine Biology and Biotechnology, Qingdao Marine Science and Technology Center, Qingdao266237, China
  4.Jiangsu Innovation Center of Marine Bioresources, Jiangsu Coast Development Investment Co., Ltd., Jiangsu Coast Development Group Co., Ltd., Nanjing210019, China
- Author bio：
  
  huyadong@jsyhkf.com
  jingwang@qdio.ac.cn
- Funds：
- DOI：10.1007/s00343-024-4042-8
  CLC：
- Received：06 February 2024，
  
  Published：01 November 2024
- Accepted：
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YAN Changhui,PAN Mingxuan,GENG Lihua,et al.Research Paper A novel enzyme-assisted one-pot method for the extraction of fucoidan and alginate oligosaccharides from Lessonia trabeculata and their bioactivities[J].Journal of Oceanology and Limnology,2024,42(06):1998-2012.
DOI：

YAN Changhui,PAN Mingxuan,GENG Lihua,et al.Research Paper A novel enzyme-assisted one-pot method for the extraction of fucoidan and alginate oligosaccharides from Lessonia trabeculata and their bioactivities[J].Journal of Oceanology and Limnology,2024,42(06):1998-2012. DOI： 10.1007/s00343-024-4042-8.

摘要

Abstract

Alginate oligosaccharides (AOS) and fucoidan are two important bioactive carbohydrate which that can be prepared from brown seaweeds. We investigated a novel enzyme-assisted one-pot method on AOS and fucoidan extraction from

Lessonia

trabeculata

and found that the optimum extraction procedure was: first

using 10-g/L Na

solution to digest the seaweeds for 2 h at 80 °‍‍C with the material-liquid ratio 1꞉20 (g/mL)

and then adding 4.0% fucoidan cleavage enzyme for reaction for 12 h at 45 °‍‍C. The yield of AOS and fucoidan was 21.36% and 14.40%

respectively

which was higher than those

using traditional methods. The degree of polymerization of AOS was determined to be 3–5

confirming the results through thin layer chromatography (TLC) and electrospray ionization mass spectrometry (ESI-MS). The ratio of guluronic acid to mannuronic acid in AOS was found to be 1.97. Through anion-exchange column chromatography

four sulfated polysaccharide fractions (F0.5

F1.0

F1.5

and F2.0) were successfully isolated from fucoidan. Chemical analysis revealed that fucoidan and F0.5 were heteropolysaccharides

consisting mainly of mannuronic acid

fucose

and xylose

while the other fractions were predominantly composed of fucose and galactose. The sulfate group content increased from 8.74% to 18.76%

while the uronic acid content decreased from 23.79% to 5.58% across the fractions. All fractions of fucoidan exhibited significant antioxidant activity in various tests. There was a positive correlation between the sulfate content and the ability to scavenge superoxide radicals. Additionally

both AOS and the fucoidan fractions showed immunostimulation activity and were non-toxic to RAW264.7 cells. These results indicate that the novel enzyme-assisted one-pot method is a green and efficient approach for the extraction of fucoidan and alginate oligosaccharides from brown seaweeds. It has potential applications in the processing industry of brown seaweeds.

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references

Aarstad O A , Tøndervik A , Sletta H et al . 2012 . Alginate sequencing: an analysis of block distribution in alginates using specific alginate degrading enzymes . Biomacromolecules , 13 ( 1 ): 106 - 116 , https://doi.org/10.1021/bm2013026 https://doi.org/10.1021/bm2013026 .

Abraham R E , Su P , Puri M et al . 2019 . Optimisation of biorefinery production of alginate, fucoidan and laminarin from brown seaweed Durvillaea potatorum . Algal Research , 38 : 101389 , https://doi.org/10.1016/j.algal.2018.101389 https://doi.org/10.1016/j.algal.2018.101389 .

Abuduwaili A , Rozi P , Mutailifu P et al . 2019 . Effects of different extraction techniques on physicochemical properties and biological activities of polysaccharides from Fritillaria pallidiflora Schrenk . Process Biochemistry , 83 : 189 - 197 , https://doi.org/10.1016/j.procbio.2019.05.020 https://doi.org/10.1016/j.procbio.2019.05.020 .

Acosta-Estrada B A , Gutiérrez-Uribe J A , Serna-Saldívar S O . 2014 . Bound phenolics in foods, a review . Food Chemistry , 152 : 46 - 55 , https://doi.org/10.1016/j.foodchem.2013.11.093 https://doi.org/10.1016/j.foodchem.2013.11.093 .

Al Monla R , Dassouki Z , Sari-Chmayssem N et al . 2022 . Fucoidan and alginate from the brown algae Colpomenia sinuosa and their combination with vitamin C trigger apoptosis in colon cancer . Molecules , 27 ( 2 ): 358 , https://doi.org/10.3390/molecules27020358 https://doi.org/10.3390/molecules27020358 .

Ale M T , Mikkelsen J D , Meyer A S . 2011 . Important determinants for fucoidan bioactivity: a critical review of structure-function relations and extraction methods for fucose-containing sulfated polysaccharides from brown seaweeds . Marine Drugs , 9 ( 10 ): 2106 - 2130 , https://doi.org/10.3390/md9102106 https://doi.org/10.3390/md9102106 .

Bitter T , Muir H M . 1962 . A modified uronic acid carbazole reaction . Analytical Biochemistry , 4 ( 4 ): 330 - 334 , https://doi.org/10.1016/0003-2697(62)90095-7 https://doi.org/10.1016/0003-2697(62)90095-7 .

Bradford M . 1976 . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding . Analytical Biochemistry , 72 ( 1-2 ): 248 - 254 , https://doi.org/10.1016/0003-2697(76)90527-3 https://doi.org/10.1016/0003-2697(76)90527-3 .

Chen X , Zhang B , Du W Q et al . 2020 . Comparison of different extraction methods for polysaccharides from Crataegus pinnatifida Bunge . International Journal of Biological Macromolecules , 150 : 1011 - 1019 , https://doi.org/10.1016/j.ijbiomac.2019.11.056 https://doi.org/10.1016/j.ijbiomac.2019.11.056 .

Chen Y J , Dou W F , Li H et al . 2018 . The alginate lyase from Isoptericola halotolerans CGMCC 5336 as a new tool for the production of alginate oligosaccharides with guluronic acid as reducing end . Carbohydrate Research , 470 : 36 - 41 , https://doi.org/10.1016/j.carres.2018.06.005 https://doi.org/10.1016/j.carres.2018.06.005 .

Cho M L , Lee B Y , You S G . 2010 . Relationship between oversulfation and conformation of low and high molecular weight fucoidans and evaluation of their in vitro anticancer activity . Molecules , 16 ( 1 ): 291 - 297 , https://doi.org/10.3390/molecules16010291 https://doi.org/10.3390/molecules16010291 .

Cunha L , Grenha A . 2016 . Sulfated seaweed polysaccharides as multifunctional materials in drug delivery applications . Marine Drugs , 14 ( 3 ): 42 , https://doi.org/10.3390/md14030042 https://doi.org/10.3390/md14030042 .

Daub C D , Mabate B , Malgas S et al . 2020 . Fucoidan from Ecklonia maxima is a powerful inhibitor of the diabetes-related enzyme, α-glucosidase . International Journal of Biological Macromolecules , 151 : 412 - 420 , https://doi.org/10.1016/j.ijbiomac.2020.02.161 https://doi.org/10.1016/j.ijbiomac.2020.02.161 .

Dong Y , Lin H Q , Miao S et al . 2021 . Advances in enzymatic extraction of polysaccharides . Science and Technology of Food Industry , 42 ( 3 ): 351 - 358 , https://doi.org/10.13386/j.issn1002-0306.2020050016. https://doi.org/10.13386/j.issn1002-0306.2020050016. (in Chinese with English abstract)

Du J , Wang S . 2018 . Progress in the research of polysaccharide extraction . Chinese Community Doctors , 34 ( 17 ): 9 , 11 , https://doi.org/10.3969/j.issn.1007-614x.2018.17.003. https://doi.org/10.3969/j.issn.1007-614x.2018.17.003. (in Chinese with English abstract)

Dubois M , Gilles K A , Hamilton J K et al . 1956 . Colorimetric method for determination of sugars and related substances . Analytical Chemistry , 28 ( 3 ): 350 - 356 , https://doi.org/10.1021/ac60111a017 https://doi.org/10.1021/ac60111a017 .

Franz S , Rammelt S , Scharnweber D et al . 2011 . Immune responses to implants—a review of the implications for the design of immunomodulatory biomaterials . Biomaterials , 32 ( 28 ): 6692 - 6709 , https://doi.org/10.1016/j.biomaterials.2011.05.078 https://doi.org/10.1016/j.biomaterials.2011.05.078 .

Furman D , Campisi J , Verdin E et al . 2019 . Chronic inflammation in the etiology of disease across the life span . Nature Medicine , 25 ( 12 ): 1822 - 1832 , https://doi.org/10.1038/s41591-019-0675-0 https://doi.org/10.1038/s41591-019-0675-0 .

Ganapathy S , Lingappa S , Naidu K et al . 2019 . Isolation and bioactive potential of fucoidan from marine macroalgae Turbinaria conoides . Chemistry Select , 4 ( 48 ): 14114 - 14119 , https://doi.org/10.1002/slct.201903548 https://doi.org/10.1002/slct.201903548 .

Geng L H , Hu W C , Liu Y J et al . 2018a . A heteropolysaccharide from Saccharina japonica with immunomodulatory effect on RAW 264.7 cells . Carbohydrate Polymers , 201 : 557 - 565 , https://doi.org/10.1016/j.carbpol.2018.08.096 https://doi.org/10.1016/j.carbpol.2018.08.096 .

Geng L H , Zhang Q B , Wang J et al . 2018b . Glucofucogalactan, a heterogeneous low-sulfated polysaccharide from Saccharina japonica and its bioactivity . International Journal of Biological Macromolecules , 113 : 90 - 97 , https://doi.org/10.1016/j.ijbiomac.2018.02.008 https://doi.org/10.1016/j.ijbiomac.2018.02.008 .

Green L C , Wagner D A , Glogowski J et al . 1982 . Analysis of nitrate, nitrite, and [ 15 N ] nitrate in biological fluids. Analytical Biochemistry , 126 ( 1 ): 131 - 138 , https://doi.org/10.1016/0003-2697(82)90118-X https://doi.org/10.1016/0003-2697(82)90118-X .

Grice H C . 1988 . Safety evaluation of butylated hydroxyanisole from the perspective of effects on forestomach and oesophageal squamous epithelium . Food and Chemical Toxicology , 26 ( 8 ): 717 - 723 , https://doi.org/10.1016/0278-6915(88)90072-5 https://doi.org/10.1016/0278-6915(88)90072-5 .

Guo Q W , Ma Q Q , Xue Z H et al . 2018 . Studies on the binding characteristics of three polysaccharides with different molecular weight and flavonoids from corn silk ( Maydis stigma ) . Carbohydrate Polymers , 198 : 581 - 588 , https://doi.org/10.1016/j.carbpol.2018.06.120 https://doi.org/10.1016/j.carbpol.2018.06.120 .

Hwang P A , Chien S Y , Chan Y L et al . 2011 . Inhibition of lipopolysaccharide (LPS)-induced inflammatory responses by Sargassum hemiphyllum sulfated polysaccharide extract in RAW 264.7 macrophage cells . Journal of Agricultural and Food Chemistry , 59 ( 5 ): 2062 - 2068 , https://doi.org/10.1021/jf1043647 https://doi.org/10.1021/jf1043647 .

Leyton A , Pezoa-Conte R , Barriga A et al . 2016 . Identification and efficient extraction method of phlorotannins from the brown seaweed Macrocystis pyrifera using an orthogonal experimental design . Algal Research , 16 : 201 - 208 , https://doi.org/10.1016/j.algal.2016.03.019 https://doi.org/10.1016/j.algal.2016.03.019 .

Li T . 2015 . Research on pectin extraction process from apple pomace . Tianjin Agricultural Sciences , 21 ( 1 ): 18 - 21 , 25, https://doi.org/10.3969/j.issn.‍1006-6500,2014.01. 005 . (in Chinese)

Li Y J , Fan J X , Cheng L K et al . 2017 . Co-extraction and characterization of alginate and fucoidan from two Sargassum fusiforme strains . Science and Technology of Food Industry , 38 ( 6 ): 90 - 95 , 100 , https://doi.org/10.13386/j.issn1002-0306.2017.06.008. https://doi.org/10.13386/j.issn1002-0306.2017.06.008. (in Chinese with English abstract)

Liu H , Chen S J , Yang X Q . 2018 . Advances of extraction, purification and application of polysaccharides from seaweeds . Science and Technology of Food Industry , 39 ( 12 ): 341 - 346 , https://doi.org/10.13386/j.issn1002-0306.2018.12.061. https://doi.org/10.13386/j.issn1002-0306.2018.12.061. (in Chinese with English abstract)

Liu J , Yang S Q , Li X T et al . 2019 . Alginate oligosaccharides: production, biological activities, and potential applications . Comprehensive Reviews in Food Science and Food Safety , 18 ( 6 ): 1859 - 1881 , https://doi.org/10.1111/1541-4337.12494 https://doi.org/10.1111/1541-4337.12494 .

Lu L B , Zhang P , Cao Y et al . 2009 . Study on partially oxidized sodium alginate with potassium permanganate as the oxidant . Journal of Applied Polymer Science , 113 ( 6 ): 3585 - 3589 , https://doi.org/10.1002/app.30328 https://doi.org/10.1002/app.30328 .

Mohamed S , Hashim S N , Rahman H A . 2012 . Seaweeds: a sustainable functional food for complementary and alternative therapy . Trends in Food Science & Technology , 23 ( 2 ): 83 - 96 , https://doi.org/10.1016/j.tifs.2011.09.001 https://doi.org/10.1016/j.tifs.2011.09.001 .

Morya V K , Kim J , Kim E K . 2012 . Algal fucoidan: structural and size-dependent bioactivities and their perspectives . Applied Microbiology and Biotechnology , 93 ( 1 ): 71 - 82 , https://doi.org/10.1007/s00253-011-3666-8 https://doi.org/10.1007/s00253-011-3666-8 .

Mosmann T . 1983 . Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays . Journal of Immunological Methods , 65 ( 1-2 ): 55 - 63 , https://doi.org/10.1016/0022-1759(83)90303-4 https://doi.org/10.1016/0022-1759(83)90303-4 .

Murúa P , Westermeier R , Patiño D J et al . 2013 . Culture studies on early development of Lessonia trabeculata (Phaeophyceae, Laminariales): seasonality and acclimation to light and temperature . Phycological Research , 61 ( 2 ): 145 - 153 , https://doi.org/10.1111/pre.12013 https://doi.org/10.1111/pre.12013 .

Nadar S S , Rao P , Rathod V K . 2018 . Enzyme assisted extraction of biomolecules as an approach to novel extraction technology: a review . Food Research International , 108 : 309 - 330 , https://doi.org/10.1016/j.foodres.2018.03.006 https://doi.org/10.1016/j.foodres.2018.03.006 .

Nagahawatta D P , Liyanage N M , Jayawardhana H H A C K et al . 2022 . Anti-fine dust effect of fucoidan extracted from Ecklonia maxima leaves in macrophages via inhibiting inflammatory signaling pathways . Marine Drugs , 20 ( 7 ): 413 , https://doi.org/10.3390/md20070413 https://doi.org/10.3390/md20070413 .

Nep E I , Conway B R . 2011 . Physicochemical characterization of grewia polysaccharide gum: effect of drying method . Carbohydrate Polymers , 84 ( 1 ): 446 - 453 , https://doi.org/10.1016/j.carbpol.2010.12.005 https://doi.org/10.1016/j.carbpol.2010.12.005 .

Niemelä K , Sjöström E . 1985 . Alkaline degradation of alginates to carboxylic acids . Carbohydrate Research , 144 ( 2 ): 241 - 249 , https://doi.org/10.1016/S0008-6215(00)90672-4 https://doi.org/10.1016/S0008-6215(00)90672-4 .

Qu G Y , Liu X , Wang D F et al . 2014 . Isolation and characterization of fucoidans from five brown algae and evaluation of their antioxidant activity . Journal of Ocean University of China , 13 ( 5 ): 851 - 856 , https://doi.org/10.1007/s11802-014-2260-y https://doi.org/10.1007/s11802-014-2260-y .

Razmkhah S , Razavi S M A , Mohammadifar M A et al . 2016 . Protein-free cress seed ( Lepidium sativum ) gum: physicochemical characterization and rheological properties . Carbohydrate Polymers , 153 : 14 - 24 , https://doi.org/10.1016/j.carbpol.2016.07.086 https://doi.org/10.1016/j.carbpol.2016.07.086 .

Togashi H , Kato A , Shimizu K . 2009 . Enzymatically derived aldouronic acids from Eucalyptus globulus glucuronoxylan . Carbohydrate Polymers , 78 ( 2 ): 247 - 252 , https://doi.org/10.1016/j.carbpol.2009.03.035 https://doi.org/10.1016/j.carbpol.2009.03.035 .

Urtuvia V , Maturana N , Acevedo F et al . 2017 . Bacterial alginate production: an overview of its biosynthesis and potential industrial production . World Journal of Microbiology and Biotechnology , 33 ( 11 ): 198 , https://doi.org/10.1007/s11274-017-2363-x https://doi.org/10.1007/s11274-017-2363-x .

Vasudevan U M , Lee O K , Lee E Y . 2021 . Alginate derived functional oligosaccharides: recent developments, barriers, and future outlooks . Carbohydrate Polymers , 267 : 118158 , https://doi.org/10.1016/j.carbpol.2021.118158 https://doi.org/10.1016/j.carbpol.2021.118158 .

Wang J , Zhang Q B , Zhang Z S et al . 2010 . Potential antioxidant and anticoagulant capacity of low molecular weight fucoidan fractions extracted from Laminaria japonica . International Journal of Biological Macromolecules , 46 ( 1 ): 6 - 12 , https://doi.org/10.1016/j.ijbiomac.2009.10.015 https://doi.org/10.1016/j.ijbiomac.2009.10.015 .

Wang M M , Liu Y , Qiang M L et al . 2017 . Structural elucidation of a pectin-type polysaccharide from Hovenia dulcis peduncles and its proliferative activity on RAW264.7 cells . International Journal of Biological Macromolecules , 104 : 1246 - 1253 , https://doi.org/10.1016/j.ijbiomac.2017.07.004 https://doi.org/10.1016/j.ijbiomac.2017.07.004 .

Wang X M , Zhang Z S , Yao Z Y et al . 2013 . Sulfation, anticoagulant and antioxidant activities of polysaccharide from green algae Enteromorpha linza . International Journal of Biological Macromolecules , 58 : 225 - 230 , https://doi.org/10.1016/j.ijbiomac.2013.04.005 https://doi.org/10.1016/j.ijbiomac.2013.04.005 .

Wardhani D H , Aryanti N , Aziz A et al . 2021 . Ultrasonic degradation of alginate: a matrix for iron encapsulation using gelation . Food Bioscience , 41 : 100803 , https://doi.org/10.1016/j.fbio.2020.100803 https://doi.org/10.1016/j.fbio.2020.100803 .

Wasikiewicz J M , Yoshii F , Nagasawa N et al . 2005 . Degradation of chitosan and sodium alginate by gamma radiation, sonochemical and ultraviolet methods . Radiation Physics and Chemistry , 73 ( 5 ): 287 - 295 , https://doi.org/10.1016/j.radphyschem.2004.09.021 https://doi.org/10.1016/j.radphyschem.2004.09.021 .

Westermeier R , Patiño D , Piel M I et al . 2006 . A new approach to kelp mariculture in Chile: production of free-floating sporophyte seedlings from gametophyte cultures of Lessonia trabeculata and Macrocystis pyrifera . Aquaculture Research , 37 ( 2 ): 164 - 171 , https://doi.org/10.1111/j.1365-2109.2005.01414.x https://doi.org/10.1111/j.1365-2109.2005.01414.x .

Xiao Y H , Liu S C , Shen M Y et al . 2019 . Physicochemical, rheological and thermal properties of Mesona chinensis polysaccharides obtained by sodium carbonate assisted and cellulase assisted extraction . International Journal of Biological Macromolecules , 126 : 30 - 36 , https://doi.org/10.1016/j.ijbiomac.2018.12.211 https://doi.org/10.1016/j.ijbiomac.2018.12.211 .

Xing M C , Cao Q , Wang Y et al . 2020 . Advances in research on the bioactivity of alginate oligosaccharides . Marine Drugs , 18 ( 3 ): 144 , https://doi.org/10.3390/md18030144 https://doi.org/10.3390/md18030144 .

Yang L , Chen W N , Zheng J X et al . 2021 . Extraction, isolation and purification of seaweed polysaccharide and its application in food industry . Science and Technology of Food Industry , 42 ( 9 ): 365 - 372 , https://doi.org/10.13386/j.issn1002-0306.2019110241. https://doi.org/10.13386/j.issn1002-0306.2019110241. (in Chinese with English abstract)

Yang Z , Li J P , Guan H S . 2004 . Preparation and characterization of oligomannuronates from alginate degraded by hydrogen peroxide . Carbohydrate Polymers , 58 ( 2 ): 115 - 121 , https://doi.org/10.1016/j.carbpol.2004.04.022 https://doi.org/10.1016/j.carbpol.2004.04.022 .

Yuan Y , Macquarrie D J . 2015 . Microwave assisted step-by-step process for the production of fucoidan, alginate sodium, sugars and biochar from Ascophyllum nodosum through a biorefinery concept . Bioresource Technology , 198 : 819 - 827 , https://doi.org/10.1016/j.biortech.2015.09.090 https://doi.org/10.1016/j.biortech.2015.09.090 .

Zhang C G , Wang W X , Zhao X M et al . 2020 . Preparation of alginate oligosaccharides and their biological activities in plants: a review . Carbohydrate Research , 494 : 108056 , https://doi.org/10.‍1016/j.carres.2020.108056 https://doi.org/10.‍1016/j.carres.2020.108056 .

Zhang Y , Kong X Y , Wang Z M et al . 2018 . Optimization of enzymatic hydrolysis for effective lipid extraction from microalgae Scenedesmus sp . Renewable Energy , 125 : 1049 - 1057 , https://doi.org/10.1016/j.renene.2018.01.078 https://doi.org/10.1016/j.renene.2018.01.078 .

Zhang Y H , Song X N , Lin Y et al . 2019 . Antioxidant capacity and prebiotic effects of Gracilaria neoagaro oligosaccharides prepared by agarase hydrolysis . International Journal of Biological Macromolecules , 137 : 177 - 186 , https://doi.org/10.1016/j.ijbiomac.2019.06.207 https://doi.org/10.1016/j.ijbiomac.2019.06.207 .

Zhu B W , Huang L S X , Tan H D et al . 2015 . Characterization of a new endo-type polyM-specific alginate lyase from Pseudomonas sp . Biotechnology Letters , 37 ( 2 ): 409 - 415 , https://doi.org/10.1007/s10529-014-1685-0 https://doi.org/10.1007/s10529-014-1685-0 .

Zhu B W , Ni F , Xiong Q et al . 2021 . Marine oligosaccharides originated from seaweeds: source, preparation, structure, physiological activity and applications . Critical Reviews in Food Science and Nutrition , 61 ( 1 ): 60 - 74 , https://doi.org/10.1080/10408398.2020.1716207 https://doi.org/10.1080/10408398.2020.1716207 .

Zimoch-Korzycka A , Kulig D , Król-Kilińska Ż et al . 2021 . Biophysico-chemical properties of alginate oligomers obtained by acid and oxidation depolymerization . Polymers , 13 ( 14 ): 2258 , https://doi.org/10.3390/polym13142258 https://doi.org/10.3390/polym13142258 .

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Related Author

Qishan SUO

Zhenzhen DENG

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Quanbin ZHANG

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University of Chinese Academy of Sciences

Nantong Zhongke Marine Science and Technology Research and Development Center

Laboratory for Marine Drugs and Biological Products, National Laboratory for Marine Science and Technology

Department of Pharmaceutical Engineering, Qingdao University of Science and Technology

Laboratory for Marine Biology and Biotechnology, National Laboratory for Marine Science and Technology

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A novel enzyme-assisted one-pot method for the extraction of fucoidan and alginate oligosaccharides from Lessonia trabeculata and their bioactivities

DOI：10.1007/s00343-024-4042-8

摘要

Abstract

关键词

Keywords

references