Comparative transcriptomic analysis of ovary and testis reveals sex-related genes in roughskin sculpin <italic style="font-style: italic">Trachidermus</italic> <italic style="font-style: italic">fasciatus</italic>

Dongxiu XUE; Sudong XIA; Tengfei XING; Yuqian GAO; Jinxian LIU

doi:10.1007/s00343-024-3285-8

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Comparative transcriptomic analysis of ovary and testis reveals sex-related genes in roughskin sculpin Trachidermus fasciatus

Biology | Updated：2024-11-19

- Comparative transcriptomic analysis of ovary and testis reveals sex-related genes in roughskin sculpin Trachidermus fasciatus
- Journal of Oceanology and Limnology Vol. 42, Issue 5, Pages: 1673-1682(2024)
- Affiliations：
  
  1.CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao266071, China
  2.Laboratory for Marine Ecology and Environmental Science, Qingdao Marine Science and Technology Center, Qingdao266200, China
  3.Tianjin Key Lab of Aqua-Ecology and Aquaculture, Department of Fishery Science, Tianjin300380, China
- Author bio：
  
  xuedongxiu@qdio.ac.cn
- Funds：
- DOI：10.1007/s00343-024-3285-8
  CLC：
- Received：26 December 2023，
  
  Online First：18 February 2024，
  
  Published：01 September 2024
- Accepted：
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XUE Dongxiu,XIA Sudong,XING Tengfei,et al.Comparative transcriptomic analysis of ovary and testis reveals sex-related genes in roughskin sculpin Trachidermus fasciatus[J].Journal of Oceanology and Limnology,2024,42(05):1673-1682.
DOI：

XUE Dongxiu,XIA Sudong,XING Tengfei,et al.Comparative transcriptomic analysis of ovary and testis reveals sex-related genes in roughskin sculpin Trachidermus fasciatus[J].Journal of Oceanology and Limnology,2024,42(05):1673-1682. DOI： 10.1007/s00343-024-3285-8.

摘要

Abstract

The mechanisms underlying sex determination and differentiation have long intrigued researchers in the fields of development and evolutionary biology. The roughskin sculpin (

Trachidermus

fasciatus

Heckel)

displaying sexual dimorphism

provides an ideal model for studying the mechanisms. However

both genetic and genomic information concerning sex determination and differentiation

such as gonadal transcriptome data in roughskin sculpin

are lacking. Here

we present the first gonadal transcriptomes of roughskin sculpin and identify sex-related genes. We identified 8 531 differentially expressed genes (DEGs)

among them 4 065 were upregulated in the ovary and 4 466 upregulated in the testis. Several sex-related gene ontology (GO) terms were enriched in ovary-biased genes

including “binding of sperm to zona pellucida”

“egg coat formation”

“positive regulation of acrosome reaction”

“cell division”

and “cell cycle”

while the GO terms such as “spermatogenesis”

“sperm axoneme assembly”

“cilium assembly”

“cilium movement”

and “cilium movement involved in cell motility” were enriched in testis-biased genes. Moreover

six KEGG pathways were significantly enriched in the ovary

whereas only one was enriched in the testis. Of these DEGs

40 sex-related genes were identified

which including 26 testis-biased genes (such as

Dmrtb1

Gsdf

Sox9b

Wnt4b

Tcp11l2

and

Efhb

)

and 14 ovary-biased genes (such as

Cyp19a1a

Foxh1

Foxr1

Gdf3

Hsd17b12

and

Igf2bp3

). This gonadal transcript dataset would broaden our understanding of sex determination and differentiation mechanisms in roughskin sculpin

expand the genomic database

support future studies on sex-related gene functions

and facilitate molecular biology research into roughskin sculpin.

关键词

Keywords

references

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Comparative transcriptomic analysis of ovary and testis reveals sex-related genes in roughskin sculpin Trachidermus fasciatus

DOI：10.1007/s00343-024-3285-8

摘要

Abstract

关键词

Keywords

references