Molecular cloning and functional analysis of two calcium-associated cuticular protein genes in <italic style="font-style: italic">Neocaridina</italic> <italic style="font-style: italic">denticulata</italic> <italic style="font-style: italic">sinensis</italic>

Xi GAO; Mengmeng DUAN; Yuying SUN; Jiquan ZHANG

doi:10.1007/s00343-024-3211-0

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Molecular cloning and functional analysis of two calcium-associated cuticular protein genes in Neocaridina denticulata sinensis

Biology | Updated：2024-11-19

- Molecular cloning and functional analysis of two calcium-associated cuticular protein genes in Neocaridina denticulata sinensis
- Journal of Oceanology and Limnology Vol. 42, Issue 5, Pages: 1659-1672(2024)
- Affiliations：
  
  1.School of Life Sciences/Hebei Basic Science Center for Biotic Interaction, Hebei University, Baoding071002, China
  2.Institute of Life Science and Green Development, Hebei University, Baoding071002, China
- Author bio：
  
  sunyuying125@hbu.edu.cn
  zhangjiquan@hbu.edu.cn
- Funds：
- DOI：10.1007/s00343-024-3211-0
  CLC：
- Received：15 October 2023，
  
  Online First：03 February 2024，
  
  Published：01 September 2024
- Accepted：
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GAO Xi,DUAN Mengmeng,SUN Yuying,et al.Molecular cloning and functional analysis of two calcium-associated cuticular protein genes in Neocaridina denticulata sinensis[J].Journal of Oceanology and Limnology,2024,42(05):1659-1672.
DOI：

GAO Xi,DUAN Mengmeng,SUN Yuying,et al.Molecular cloning and functional analysis of two calcium-associated cuticular protein genes in Neocaridina denticulata sinensis[J].Journal of Oceanology and Limnology,2024,42(05):1659-1672. DOI： 10.1007/s00343-024-3211-0.

摘要

Abstract

The body surface of crustaceans is covered with a sturdy shell. The growth and development of crustaceans are realized through molting.

Neocaridina

denticulata

sinensis

is a suitable candidate for crustacean scientific research. Two calcium-associated cuticular protein genes

named

NdCAP

and

NdCAP

were obtained from

denticulata

sinensis

. Molecular docking simulated the binding effect of both proteins and calcium ions. Semi-quantitative reverse transcription PCR results show that

NdCAP

is expressed in D

2‍‍‍–‍4

stage

NdCAP

expressed in D

2‍‍‍–‍4

and A‍‍‍–‍B stages

and both were significantly expressed in the cephalothorax cuticle and pereiopods. Then

it was revealed that

NdCAP

and

NdCAP

are regulated by

NdEcR

-mediated 20E signaling pathways. Knockdown of

NdCAP

and

NdCAP

was observed to cause surface defects. The recombinant proteins (rNdCAP-1 and rNdCAP-2)

obtained by prokaryotic expression

had calcium-binding and chitin-binding ability

inhibited formation of calcium carbonate precipitate. These results show that calcium-associated cuticular proteins play important roles in cuticle formation and calcification.

关键词

Keywords

references

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⁰

Molecular cloning and functional analysis of two calcium-associated cuticular protein genes in Neocaridina denticulata sinensis

DOI：10.1007/s00343-024-3211-0

摘要

Abstract

关键词

Keywords

references