Environmental concentration of ammonia nitrogen induced marked changes in proteome of clam <italic style="font-style: italic">Ruditapes</italic> <italic style="font-style: italic">philippinarum</italic> in dose- and time-dependent manner

Ming CONG; Zhaoshun LI; Wenwen TIAN; Yuanmei LI; Jiasen LÜ

doi:10.1007/s00343-024-3153-6

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Environmental concentration of ammonia nitrogen induced marked changes in proteome of clam Ruditapes philippinarum in dose- and time-dependent manner

Biology | Updated：2024-11-19

- Environmental concentration of ammonia nitrogen induced marked changes in proteome of clam Ruditapes philippinarum in dose- and time-dependent manner
- Journal of Oceanology and Limnology Vol. 42, Issue 5, Pages: 1634-1650(2024)
- Affiliations：
  
  1.School of Ocean, Yantai University, Yantai264005, China
  2.School of Life Sciences, Yantai University, Yantai264005, China
- Author bio：
  
  jslv@ytu.edu.cn
- Funds：
- DOI：10.1007/s00343-024-3153-6
  CLC：
- Received：10 August 2023，
  
  Online First：04 January 2024，
  
  Published：01 September 2024
- Accepted：
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CONG Ming,LI Zhaoshun,TIAN Wenwen,et al.Environmental concentration of ammonia nitrogen induced marked changes in proteome of clam ,Ruditapes ,philippinarum in dose- and time-dependent manner[J].Journal of Oceanology and Limnology,2024,42(05):1634-1650.
DOI：

CONG Ming,LI Zhaoshun,TIAN Wenwen,et al.Environmental concentration of ammonia nitrogen induced marked changes in proteome of clam ,Ruditapes ,philippinarum in dose- and time-dependent manner[J].Journal of Oceanology and Limnology,2024,42(05):1634-1650. DOI： 10.1007/s00343-024-3153-6.

摘要

Abstract

Previous studies have revealed that ammonia nitrogen has several adverse effects on clam

Ruditapes

philippinarum

. However

knowledge is lacking regarding the related proteins involved in the toxicological responses

which is vital to elucidate the underlying mechanism of ammonia nitrogen. In this study

clams

philippinarum

were exposed to ammonia nitrogen for 21 d at two environmentally relevant concentrations. The tandem mass tags approach (TMT) was applied to assay the differentially expressed proteins (DEPs) in clam gill tissues on the 3

and 21

day. Finally

a total of 7 263 proteins were identified. Bioinformatics analyses revealed that clam protein profiles changed in dose- and time-dependent manner after ammonia nitrogen exposure. We inferred that the clams may face heavy challenges after ammonia exposure

such as unbalanced gender ratio

lysosomal disease

energy lack

neurological disorders

altered glutamine metabolism

increased lipid synthesis

and impaired immunity. Variation profiles of enzyme activities of glutaminase and glutamine synthase provided direct evidence to verify the related inference from proteome data. Most of the inferred toxic effects merit further study. This study identified important proteins related to ammonia nitrogen toxicity in the clam and indicated the severe stress of marine ammonia pollution on the healthy development of mollusc aquaculture.

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Environmental concentration of ammonia nitrogen induced marked changes in proteome of clam Ruditapes philippinarum in dose- and time-dependent manner

DOI：10.1007/s00343-024-3153-6

摘要

Abstract

关键词

Keywords

references